Matches in SemOpenAlex for { <https://semopenalex.org/work/W2900195395> ?p ?o ?g. }
- W2900195395 abstract "Abstract A high quality genome annotation greatly facilitates successful cell line engineering. Standard draft genome annotation pipelines are based largely on de novo gene prediction, homology, and RNA-Seq data. However, draft annotations can suffer from incorrectly predictions of translated sequence, incorrect splice isoforms and missing genes. Here we generated a draft annotation for the newly assembled Chinese hamster genome and used RNA-Seq, proteomics, and Ribo-Seq to experimentally annotate the genome. We identified 4,333 new proteins compared to the hamster RefSeq protein annotation and 2,503 novel translational events (e.g., alternative splices, mutations, novel splices). Finally, we used this pipeline to identify the source of translated retroviruses contaminating recombinant products from Chinese hamster ovary (CHO) cell lines, including 131 type-C retroviruses, thus enabling future efforts to eliminate retroviruses by reducing the costs incurred with retroviral particle clearance. In summary, the improved annotation provides a more accurate platform for guiding CHO cell line engineering, including facilitating the interpretation of omics data, defining of cellular pathways, and engineering of complex phenotypes." @default.
- W2900195395 created "2018-11-16" @default.
- W2900195395 creator A5000303243 @default.
- W2900195395 creator A5002420690 @default.
- W2900195395 creator A5007558979 @default.
- W2900195395 creator A5014335552 @default.
- W2900195395 creator A5014510444 @default.
- W2900195395 creator A5018053019 @default.
- W2900195395 creator A5019048196 @default.
- W2900195395 creator A5033274018 @default.
- W2900195395 creator A5046382890 @default.
- W2900195395 creator A5054278526 @default.
- W2900195395 creator A5057259896 @default.
- W2900195395 creator A5066650872 @default.
- W2900195395 creator A5070489124 @default.
- W2900195395 creator A5073646789 @default.
- W2900195395 creator A5074538839 @default.
- W2900195395 date "2018-11-12" @default.
- W2900195395 modified "2023-09-27" @default.
- W2900195395 title "Proteogenomic annotation of the Chinese hamster reveals extensive novel translation events and endogenous retroviral elements" @default.
- W2900195395 cites W1561855928 @default.
- W2900195395 cites W1632340368 @default.
- W2900195395 cites W1889987954 @default.
- W2900195395 cites W1967734782 @default.
- W2900195395 cites W1973001000 @default.
- W2900195395 cites W1973095624 @default.
- W2900195395 cites W1973677250 @default.
- W2900195395 cites W1979631228 @default.
- W2900195395 cites W1984068087 @default.
- W2900195395 cites W1993158258 @default.
- W2900195395 cites W2002125267 @default.
- W2900195395 cites W2004291887 @default.
- W2900195395 cites W2009702030 @default.
- W2900195395 cites W2011657487 @default.
- W2900195395 cites W2019126169 @default.
- W2900195395 cites W2021653707 @default.
- W2900195395 cites W2024954384 @default.
- W2900195395 cites W2026433130 @default.
- W2900195395 cites W2027974246 @default.
- W2900195395 cites W2028673251 @default.
- W2900195395 cites W2037199116 @default.
- W2900195395 cites W2054254276 @default.
- W2900195395 cites W2056499061 @default.
- W2900195395 cites W2068198992 @default.
- W2900195395 cites W2072880634 @default.
- W2900195395 cites W2079517684 @default.
- W2900195395 cites W2093343938 @default.
- W2900195395 cites W2102278945 @default.
- W2900195395 cites W2102619694 @default.
- W2900195395 cites W2104005232 @default.
- W2900195395 cites W2106678197 @default.
- W2900195395 cites W2108234281 @default.
- W2900195395 cites W2113205564 @default.
- W2900195395 cites W2113224683 @default.
- W2900195395 cites W2117763379 @default.
- W2900195395 cites W2124779706 @default.
- W2900195395 cites W2127173397 @default.
- W2900195395 cites W2129148077 @default.
- W2900195395 cites W2131271579 @default.
- W2900195395 cites W2136563184 @default.
- W2900195395 cites W2136826997 @default.
- W2900195395 cites W2141165178 @default.
- W2900195395 cites W2160177274 @default.
- W2900195395 cites W2169456326 @default.
- W2900195395 cites W2169769306 @default.
- W2900195395 cites W2201800325 @default.
- W2900195395 cites W2262417550 @default.
- W2900195395 cites W2292054199 @default.
- W2900195395 cites W2307191250 @default.
- W2900195395 cites W2313606242 @default.
- W2900195395 cites W2461798152 @default.
- W2900195395 cites W2567961709 @default.
- W2900195395 cites W2574503332 @default.
- W2900195395 cites W2603127718 @default.
- W2900195395 cites W2623490906 @default.
- W2900195395 cites W2752477785 @default.
- W2900195395 cites W2765517019 @default.
- W2900195395 cites W2800755544 @default.
- W2900195395 cites W2804667211 @default.
- W2900195395 cites W2805084391 @default.
- W2900195395 cites W2887393058 @default.
- W2900195395 cites W4233120011 @default.
- W2900195395 cites W4236236547 @default.
- W2900195395 doi "https://doi.org/10.1101/468181" @default.
- W2900195395 hasPublicationYear "2018" @default.
- W2900195395 type Work @default.
- W2900195395 sameAs 2900195395 @default.
- W2900195395 citedByCount "0" @default.
- W2900195395 crossrefType "posted-content" @default.
- W2900195395 hasAuthorship W2900195395A5000303243 @default.
- W2900195395 hasAuthorship W2900195395A5002420690 @default.
- W2900195395 hasAuthorship W2900195395A5007558979 @default.
- W2900195395 hasAuthorship W2900195395A5014335552 @default.
- W2900195395 hasAuthorship W2900195395A5014510444 @default.
- W2900195395 hasAuthorship W2900195395A5018053019 @default.
- W2900195395 hasAuthorship W2900195395A5019048196 @default.
- W2900195395 hasAuthorship W2900195395A5033274018 @default.
- W2900195395 hasAuthorship W2900195395A5046382890 @default.
- W2900195395 hasAuthorship W2900195395A5054278526 @default.
- W2900195395 hasAuthorship W2900195395A5057259896 @default.