Matches in SemOpenAlex for { <https://semopenalex.org/work/W2900419524> ?p ?o ?g. }
- W2900419524 abstract "Studying the affinity and the kinetics of biomolecular interactions is an essential task in biology and pharmacology. Nonequilibrium capillary electrophoresis of equilibrium mixtures (NECEEM) is a promising technique that allows the measurement of the equilibrium dissociation constant (Kd), and the rate constant of dissociation (koff), and can also be used as the partitioning method in selection of aptamers. There are three critical issues that have hindered the wide adoption of NECEEM by the analytical community: (1) poor resilience of the method against systematic errors, (2) limited versatility in terms of emulating physiological conditions, and (3) restricted partitioning efficiency in DNA aptamer selection. The goals of my research project were (i) to develop strategies for minimizing the systematic errors in NECEEM; (ii) to make NECEEM compatible with the use of physiological buffers; and (iii) to eliminate the restricted partitioning efficiency of NECEEM in aptamer selection. To improve the accuracy of NECEEM, I have developed an approach for simultaneous determination of both the Kd and the concentration of one of the interacting molecules, which eliminates errors caused by the presence of improperly folded molecules in samples. Further, I have developed an algorithmic approach for optimization of NECEEM, which takes the interrelation between its experimental parameters into account and minimizes the systematic error in an objective manner. To make NECEEM compatible with physiological buffers, I have uncovered the cause behind the poor detectability of DNA in phosphate buffered saline, and have created a pressure-assisted modification of NECEEM that overcomes this problem. Lastly, I have determined that the irregular electrophoretic migration of DNA in strong electric fields is caused by the ability of DNA to form unusually stable complexes with its counterions. Based on these findings, I have developed two independent approaches that increase the efficiency of NECEEM in selection of DNA aptamers." @default.
- W2900419524 created "2018-11-16" @default.
- W2900419524 creator A5065515462 @default.
- W2900419524 date "2016-11-14" @default.
- W2900419524 modified "2023-09-27" @default.
- W2900419524 title "Improving the Accuracy, Versatility, and Partitioning Efficiency of Nonequilibrium Capillary Electrophoresis of Equilibrium Mixtures" @default.
- W2900419524 cites W1498065391 @default.
- W2900419524 cites W1506057870 @default.
- W2900419524 cites W1566656900 @default.
- W2900419524 cites W1581586115 @default.
- W2900419524 cites W1592971061 @default.
- W2900419524 cites W1964636831 @default.
- W2900419524 cites W1968463331 @default.
- W2900419524 cites W1970252429 @default.
- W2900419524 cites W1972321784 @default.
- W2900419524 cites W1973599893 @default.
- W2900419524 cites W1974572836 @default.
- W2900419524 cites W1976260744 @default.
- W2900419524 cites W1976830444 @default.
- W2900419524 cites W1976957718 @default.
- W2900419524 cites W1977260380 @default.
- W2900419524 cites W1980349612 @default.
- W2900419524 cites W1984623458 @default.
- W2900419524 cites W1984810098 @default.
- W2900419524 cites W1988813095 @default.
- W2900419524 cites W1991816010 @default.
- W2900419524 cites W1992237760 @default.
- W2900419524 cites W1992370748 @default.
- W2900419524 cites W1993868845 @default.
- W2900419524 cites W1995334921 @default.
- W2900419524 cites W1995440483 @default.
- W2900419524 cites W1996594258 @default.
- W2900419524 cites W1996819022 @default.
- W2900419524 cites W1997580052 @default.
- W2900419524 cites W1999435478 @default.
- W2900419524 cites W2000658458 @default.
- W2900419524 cites W2002275877 @default.
- W2900419524 cites W2004019640 @default.
- W2900419524 cites W2004364785 @default.
- W2900419524 cites W2005818688 @default.
- W2900419524 cites W2007451025 @default.
- W2900419524 cites W2007619503 @default.
- W2900419524 cites W2008040637 @default.
- W2900419524 cites W2008083246 @default.
- W2900419524 cites W2010890545 @default.
- W2900419524 cites W2014174537 @default.
- W2900419524 cites W2014238978 @default.
- W2900419524 cites W2016143331 @default.
- W2900419524 cites W2016846639 @default.
- W2900419524 cites W2018807526 @default.
- W2900419524 cites W2019255366 @default.
- W2900419524 cites W2025930615 @default.
- W2900419524 cites W2027429271 @default.
- W2900419524 cites W2028123660 @default.
- W2900419524 cites W2029220534 @default.
- W2900419524 cites W2030410834 @default.
- W2900419524 cites W2030443371 @default.
- W2900419524 cites W2030561313 @default.
- W2900419524 cites W2031148156 @default.
- W2900419524 cites W2031716651 @default.
- W2900419524 cites W2032480466 @default.
- W2900419524 cites W2034846105 @default.
- W2900419524 cites W2034880487 @default.
- W2900419524 cites W2036203264 @default.
- W2900419524 cites W2036219259 @default.
- W2900419524 cites W2040803249 @default.
- W2900419524 cites W2041521509 @default.
- W2900419524 cites W2041616107 @default.
- W2900419524 cites W2043157066 @default.
- W2900419524 cites W2043560548 @default.
- W2900419524 cites W2044601776 @default.
- W2900419524 cites W2044912124 @default.
- W2900419524 cites W2046521448 @default.
- W2900419524 cites W2047200410 @default.
- W2900419524 cites W2049035525 @default.
- W2900419524 cites W2053254549 @default.
- W2900419524 cites W2054724315 @default.
- W2900419524 cites W2055558161 @default.
- W2900419524 cites W2056256238 @default.
- W2900419524 cites W2058645840 @default.
- W2900419524 cites W2059223781 @default.
- W2900419524 cites W2059720471 @default.
- W2900419524 cites W2065615355 @default.
- W2900419524 cites W2066071698 @default.
- W2900419524 cites W2066074720 @default.
- W2900419524 cites W2066302130 @default.
- W2900419524 cites W2066601085 @default.
- W2900419524 cites W2067235442 @default.
- W2900419524 cites W2067689481 @default.
- W2900419524 cites W2067885827 @default.
- W2900419524 cites W2072195151 @default.
- W2900419524 cites W2073058586 @default.
- W2900419524 cites W2074171535 @default.
- W2900419524 cites W2075734514 @default.
- W2900419524 cites W2077013529 @default.
- W2900419524 cites W2077438174 @default.
- W2900419524 cites W2078267500 @default.
- W2900419524 cites W2080945020 @default.
- W2900419524 cites W2084590452 @default.
- W2900419524 cites W2087114433 @default.