FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2900501296> ?p ?o ?g. }

• W2900501296 abstract "Staphylococcus aureus is an opportunistic pathogen and a leading cause of nosocomial infections. It can acquire resistance to all the antibiotics that entered the clinics to date, and the World Health Organization defined it as a high-priority pathogen for research and development of new antibiotics. A deeper understanding of the genetic variability of S. aureus in clinical settings would lead to a better comprehension of its pathogenic potential and improved strategies to contrast its virulence and resistance. However, the number of comprehensive studies addressing clinical cohorts of S. aureus infections by simultaneously looking at the epidemiology, phylogenetic reconstruction, genomic characterisation, and transmission pathways of infective clones is currently low, thus limiting global surveillance and epidemiological monitoring. We applied whole-genome shotgun sequencing (WGS) to 184 S. aureus isolates from 135 patients treated in different operative units of an Italian paediatric hospital over a timespan of 3 years, including both methicillin-resistant S. aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) from different infection types. We typed known and unknown clones from their genomes by multilocus sequence typing (MLST), Staphylococcal Cassette Chromosome mec (SCCmec), Staphylococcal protein A gene (spa), and Panton-Valentine Leukocidin (PVL), and we inferred their whole-genome phylogeny. We explored the prevalence of virulence and antibiotic resistance genes in our cohort, and the conservation of genes encoding vaccine candidates. We also performed a timed phylogenetic investigation for a potential outbreak of a newly emerging nosocomial clone. The phylogeny of the 135 single-patient S. aureus isolates showed a high level of diversity, including 80 different lineages, and co-presence of local, global, livestock-associated, and hypervirulent clones. Five of these clones do not have representative genomes in public databases. Variability in the epidemiology is mirrored by variability in the SCCmec cassettes, with some novel variants of the type IV cassette carrying extra antibiotic resistances. Virulence and resistance genes were unevenly distributed across different clones and infection types, with highly resistant and lowly virulent clones showing strong association with chronic diseases, and highly virulent strains only reported in acute infections. Antigens included in vaccine formulations undergoing clinical trials were conserved at different levels in our cohort, with only a few highly prevalent genes fully conserved, potentially explaining the difficulty of developing a vaccine against S. aureus. We also found a recently diverged ST1-SCCmecIV-t127 PVL− clone suspected to be hospital-specific, but time-resolved integrative phylogenetic analysis refuted this hypothesis and suggested that this quickly emerging lineage was acquired independently by patients. Whole genome sequencing allowed us to study the epidemiology and genomic repertoire of S. aureus in a clinical setting and provided evidence of its often underestimated complexity. Some virulence factors and clones are specific of disease types, but the variability and dispensability of many antigens considered for vaccine development together with the quickly changing epidemiology of S. aureus makes it very challenging to develop full-coverage therapies and vaccines. Expanding WGS-based surveillance of S. aureus to many more hospitals would allow the identification of specific strains representing the main burden of infection and therefore reassessing the efforts for the discovery of new treatments and clinical practices." @default.
• W2900501296 created "2018-11-29" @default.
• W2900501296 creator A5015011335 @default.
• W2900501296 creator A5015590466 @default.
• W2900501296 creator A5018361621 @default.
• W2900501296 creator A5021755822 @default.
• W2900501296 creator A5023747376 @default.
• W2900501296 creator A5023870348 @default.
• W2900501296 creator A5029083455 @default.
• W2900501296 creator A5046801644 @default.
• W2900501296 creator A5047326597 @default.
• W2900501296 creator A5061103638 @default.
• W2900501296 creator A5068576885 @default.
• W2900501296 creator A5071736280 @default.
• W2900501296 creator A5075665406 @default.
• W2900501296 creator A5076835154 @default.
• W2900501296 creator A5090565505 @default.
• W2900501296 date "2018-11-13" @default.
• W2900501296 modified "2023-10-05" @default.
• W2900501296 title "Whole-genome epidemiology, characterisation, and phylogenetic reconstruction of Staphylococcus aureus strains in a paediatric hospital" @default.
• W2900501296 cites W1440840831 @default.
• W2900501296 cites W1490383390 @default.
• W2900501296 cites W1542487405 @default.
• W2900501296 cites W1813098396 @default.
• W2900501296 cites W1878404532 @default.
• W2900501296 cites W1882756642 @default.
• W2900501296 cites W1912909479 @default.
• W2900501296 cites W1949463134 @default.
• W2900501296 cites W1962185498 @default.
• W2900501296 cites W1963589824 @default.
• W2900501296 cites W1963979170 @default.
• W2900501296 cites W1965495205 @default.
• W2900501296 cites W1968660263 @default.
• W2900501296 cites W1973944724 @default.
• W2900501296 cites W1975243375 @default.
• W2900501296 cites W1976718759 @default.
• W2900501296 cites W1978413723 @default.
• W2900501296 cites W1978669886 @default.
• W2900501296 cites W1980030331 @default.
• W2900501296 cites W1980445954 @default.
• W2900501296 cites W1982944795 @default.
• W2900501296 cites W1984094310 @default.
• W2900501296 cites W1984791245 @default.
• W2900501296 cites W1985865348 @default.
• W2900501296 cites W1990011031 @default.
• W2900501296 cites W1994059767 @default.
• W2900501296 cites W1994895410 @default.
• W2900501296 cites W1995134733 @default.
• W2900501296 cites W1998221324 @default.
• W2900501296 cites W2003379483 @default.
• W2900501296 cites W2004538785 @default.
• W2900501296 cites W2004917278 @default.
• W2900501296 cites W2012232695 @default.
• W2900501296 cites W2012830590 @default.
• W2900501296 cites W2013289028 @default.
• W2900501296 cites W2013314072 @default.
• W2900501296 cites W2018238850 @default.
• W2900501296 cites W2019533660 @default.
• W2900501296 cites W2020086337 @default.
• W2900501296 cites W2028242001 @default.
• W2900501296 cites W2028784466 @default.
• W2900501296 cites W2029607174 @default.
• W2900501296 cites W2030050717 @default.
• W2900501296 cites W2034677156 @default.
• W2900501296 cites W2036544384 @default.
• W2900501296 cites W2037341401 @default.
• W2900501296 cites W2039939341 @default.
• W2900501296 cites W2040559263 @default.
• W2900501296 cites W2044678948 @default.
• W2900501296 cites W2045604845 @default.
• W2900501296 cites W2046501778 @default.
• W2900501296 cites W2048787993 @default.
• W2900501296 cites W2049347977 @default.
• W2900501296 cites W2050066382 @default.
• W2900501296 cites W2050161704 @default.
• W2900501296 cites W2055043387 @default.
• W2900501296 cites W2057387605 @default.
• W2900501296 cites W2060288448 @default.
• W2900501296 cites W2060534700 @default.
• W2900501296 cites W2061311514 @default.
• W2900501296 cites W2065630677 @default.
• W2900501296 cites W2065751779 @default.
• W2900501296 cites W2066897136 @default.
• W2900501296 cites W2068223926 @default.
• W2900501296 cites W2070457962 @default.
• W2900501296 cites W2072645998 @default.
• W2900501296 cites W2076142650 @default.
• W2900501296 cites W2077088391 @default.
• W2900501296 cites W2078078890 @default.
• W2900501296 cites W2078335862 @default.
• W2900501296 cites W2078433671 @default.
• W2900501296 cites W2079062405 @default.
• W2900501296 cites W2086165685 @default.
• W2900501296 cites W2087865186 @default.
• W2900501296 cites W2088137635 @default.
• W2900501296 cites W2088319417 @default.
• W2900501296 cites W2095960310 @default.
• W2900501296 cites W2096093282 @default.
• W2900501296 cites W2096565388 @default.
• W2900501296 cites W2097555049 @default.

• next