FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2900520926> ?p ?o ?g. }

• W2900520926 endingPage "184" @default.
• W2900520926 startingPage "176" @default.
• W2900520926 abstract "Although human islet transplantation has been established as valid and safe treatment for patients with type 1 diabetes, the utilization rates of human pancreases for clinical islet transplantation are still limited and substantially determined by the quality and composition of collagenase blends. While function and integrity of collagenase has been extensively investigated, information is still lacking about the most suitable supplementary neutral proteases. The present study compared islet isolation outcome after pancreas digestion by means of collagenase used alone or supplemented with either neutral protease (NP), clostripain (CP), or both proteases. Decent amounts of islet equivalents (IEQ) were isolated using collagenase alone (3090 ± 550 IEQ/g), or in combination with NP (2340 ± 450 IEQ/g) or CP (2740 ± 280 IEQ/g). Nevertheless, the proportion of undigested tissue was higher after using collagenase alone (21.1 ± 1.1%, P < 0.05) compared with addition of NP (13.3 ± 2.2%) or CP plus NP (13.7 ± 2.6%). Likewise, the percentage of embedded islets was highest using collagenase only (13 ± 2%) and lowest adding NP plus CP (4 ± 1%, P < 0.01). The latter combination resulted in lowest post-culture overall survival (42.7 ± 3.9%), while highest survival was observed after supplementation with CP (74.5 ± 4.8%, P < 0.01). An insulin response toward glucose challenge was present in all experimental groups, but the stimulation index was significantly decreased using collagenase plus NP (2.0 ± 0.12) compared with supplementation with CP (3.16 ± 0.4, P < 0.001). This study demonstrates for the first time that it is possible to isolate significant numbers of human islets combining collagenase only with CP. The supplementation with CP is an effective means to substantially reduce NP activity, which significantly decreases survival and viability after culture. This will facilitate the manufacturing of enzyme blends with less harmful characteristics." @default.
• W2900520926 created "2018-11-29" @default.
• W2900520926 creator A5002431454 @default.
• W2900520926 creator A5033338569 @default.
• W2900520926 creator A5035148818 @default.
• W2900520926 creator A5052117911 @default.
• W2900520926 creator A5055673489 @default.
• W2900520926 creator A5082100458 @default.
• W2900520926 date "2018-11-13" @default.
• W2900520926 modified "2023-09-24" @default.
• W2900520926 title "Comparison of Clostripain and Neutral Protease as Supplementary Enzymes for Human Islet Isolation" @default.
• W2900520926 cites W1189869135 @default.
• W2900520926 cites W1585527995 @default.
• W2900520926 cites W1967592019 @default.
• W2900520926 cites W1969089838 @default.
• W2900520926 cites W1969730253 @default.
• W2900520926 cites W1970526915 @default.
• W2900520926 cites W1979784513 @default.
• W2900520926 cites W1982378595 @default.
• W2900520926 cites W1996031046 @default.
• W2900520926 cites W1996676082 @default.
• W2900520926 cites W1998993586 @default.
• W2900520926 cites W1999228150 @default.
• W2900520926 cites W2002192212 @default.
• W2900520926 cites W2005198764 @default.
• W2900520926 cites W2010661668 @default.
• W2900520926 cites W2010948352 @default.
• W2900520926 cites W2011007681 @default.
• W2900520926 cites W2016652687 @default.
• W2900520926 cites W2019714645 @default.
• W2900520926 cites W2020018887 @default.
• W2900520926 cites W2020474241 @default.
• W2900520926 cites W2021214934 @default.
• W2900520926 cites W2021391073 @default.
• W2900520926 cites W2023202494 @default.
• W2900520926 cites W2025909864 @default.
• W2900520926 cites W2032203942 @default.
• W2900520926 cites W2034663883 @default.
• W2900520926 cites W2036022664 @default.
• W2900520926 cites W2036196131 @default.
• W2900520926 cites W2036204182 @default.
• W2900520926 cites W2036956907 @default.
• W2900520926 cites W2038628677 @default.
• W2900520926 cites W2040105970 @default.
• W2900520926 cites W2047201631 @default.
• W2900520926 cites W2048528380 @default.
• W2900520926 cites W2055142781 @default.
• W2900520926 cites W2055945368 @default.
• W2900520926 cites W2060485137 @default.
• W2900520926 cites W2066795948 @default.
• W2900520926 cites W2070288789 @default.
• W2900520926 cites W2077556526 @default.
• W2900520926 cites W2079937591 @default.
• W2900520926 cites W2092702917 @default.
• W2900520926 cites W2120433303 @default.
• W2900520926 cites W2122846793 @default.
• W2900520926 cites W2128934917 @default.
• W2900520926 cites W2135604229 @default.
• W2900520926 cites W2171678230 @default.
• W2900520926 cites W2172305142 @default.
• W2900520926 cites W2187985754 @default.
• W2900520926 cites W2320819436 @default.
• W2900520926 cites W2331768284 @default.
• W2900520926 cites W2341414956 @default.
• W2900520926 cites W2409272595 @default.
• W2900520926 cites W2524839447 @default.
• W2900520926 cites W2528516704 @default.
• W2900520926 cites W2539044895 @default.
• W2900520926 cites W2554650020 @default.
• W2900520926 cites W27028600 @default.
• W2900520926 cites W2788858792 @default.
• W2900520926 cites W2790036124 @default.
• W2900520926 cites W4235460342 @default.
• W2900520926 cites W4251808853 @default.
• W2900520926 doi "https://doi.org/10.1177/0963689718811614" @default.
• W2900520926 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6362525" @default.
• W2900520926 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30419762" @default.
• W2900520926 hasPublicationYear "2018" @default.
• W2900520926 type Work @default.
• W2900520926 sameAs 2900520926 @default.
• W2900520926 citedByCount "6" @default.
• W2900520926 countsByYear W29005209262020 @default.
• W2900520926 countsByYear W29005209262021 @default.
• W2900520926 crossrefType "journal-article" @default.
• W2900520926 hasAuthorship W2900520926A5002431454 @default.
• W2900520926 hasAuthorship W2900520926A5033338569 @default.
• W2900520926 hasAuthorship W2900520926A5035148818 @default.
• W2900520926 hasAuthorship W2900520926A5052117911 @default.
• W2900520926 hasAuthorship W2900520926A5055673489 @default.
• W2900520926 hasAuthorship W2900520926A5082100458 @default.
• W2900520926 hasBestOaLocation W29005209261 @default.
• W2900520926 hasConcept C126322002 @default.
• W2900520926 hasConcept C134018914 @default.
• W2900520926 hasConcept C165220095 @default.
• W2900520926 hasConcept C16685009 @default.
• W2900520926 hasConcept C181199279 @default.
• W2900520926 hasConcept C182220744 @default.
• W2900520926 hasConcept C185592680 @default.

• next