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- W2900580650 endingPage "272" @default.
- W2900580650 startingPage "254" @default.
- W2900580650 abstract "The synthesis and original thermoresponsive behavior of hybrid diblock copolypeptides composed of synthetic and recombinant polypeptides are herein reported. A thermoresponsive recombinant elastin-like polypeptide was used as a macroinitiator to synthesize a range of poly(l-glutamic acid)-block-elastin-like polypeptide (PGlu-b-ELP) diblock copolypeptides with variable PGlu block lengths. Their temperature-triggered self-assembly in water and in phosphate-buffered saline (PBS) was investigated at the macroscopic scale using complementary techniques such as turbidimetry, dynamic and static light scattering, small-angle neutron scattering, and at the molecular scale by 1H NMR and circular dichroism (CD). In deionized water, PGlu-b-ELP copolypeptides showed one transition from free soluble chains below the transition temperature (Tt) of the ELP block to macroscopic aggregates above the Tt. In contrast, in PBS, four successive regimes were observed upon increasing temperature: below the Tt, copolypeptides were soluble, above the Tt, large aggregates appeared and fell apart into discrete and defined spherical nanoparticles at a temperature named critical micellization temperature (CMT), before finally reaching an equilibrium. During the last regime, neutron scattering experiments revealed that the micelle-like structures underwent a densification step and expelled water from their core. In addition, 1H NMR and CD experiments revealed, in deionized water, the formation of type II β-turns into the ELP block upon temperature increase. These β-turns are known to participate in the intrinsic thermoresponsive behavior of the ELPs. In contrast, in PBS, circular dichroism measurements showed an attenuation of folded structure during the self-assembly phase, leading to less cohesive aggregates able to reorganize into nanoparticles at the CMT." @default.
- W2900580650 created "2018-11-29" @default.
- W2900580650 creator A5003736750 @default.
- W2900580650 creator A5031009436 @default.
- W2900580650 creator A5034910440 @default.
- W2900580650 creator A5036606704 @default.
- W2900580650 creator A5049063354 @default.
- W2900580650 date "2018-11-20" @default.
- W2900580650 modified "2023-10-16" @default.
- W2900580650 title "Self-Assembly of Stimuli-Responsive Biohybrid Synthetic-<i>b</i>-Recombinant Block Copolypeptides" @default.
- W2900580650 cites W1440962012 @default.
- W2900580650 cites W1823263094 @default.
- W2900580650 cites W1917316612 @default.
- W2900580650 cites W1967985930 @default.
- W2900580650 cites W1970147513 @default.
- W2900580650 cites W1971594270 @default.
- W2900580650 cites W1973724964 @default.
- W2900580650 cites W1975961988 @default.
- W2900580650 cites W1976075691 @default.
- W2900580650 cites W1979849949 @default.
- W2900580650 cites W1980419410 @default.
- W2900580650 cites W1982941857 @default.
- W2900580650 cites W1985031184 @default.
- W2900580650 cites W1987751964 @default.
- W2900580650 cites W1989489362 @default.
- W2900580650 cites W1990431921 @default.
- W2900580650 cites W1991832475 @default.
- W2900580650 cites W1996700969 @default.
- W2900580650 cites W2001080767 @default.
- W2900580650 cites W2009295373 @default.
- W2900580650 cites W2011287303 @default.
- W2900580650 cites W2013302352 @default.
- W2900580650 cites W2014269568 @default.
- W2900580650 cites W2014680193 @default.
- W2900580650 cites W2015877441 @default.
- W2900580650 cites W2016412747 @default.
- W2900580650 cites W2016747572 @default.
- W2900580650 cites W2017666052 @default.
- W2900580650 cites W2018558396 @default.
- W2900580650 cites W2024250531 @default.
- W2900580650 cites W2024377588 @default.
- W2900580650 cites W2024649234 @default.
- W2900580650 cites W2026607816 @default.
- W2900580650 cites W2029952867 @default.
- W2900580650 cites W2030383861 @default.
- W2900580650 cites W2031025647 @default.
- W2900580650 cites W2031652784 @default.
- W2900580650 cites W2033545645 @default.
- W2900580650 cites W2036391463 @default.
- W2900580650 cites W2037136982 @default.
- W2900580650 cites W2038277098 @default.
- W2900580650 cites W2041083551 @default.
- W2900580650 cites W2042424591 @default.
- W2900580650 cites W2046630837 @default.
- W2900580650 cites W2047501741 @default.
- W2900580650 cites W2056296989 @default.
- W2900580650 cites W2064341217 @default.
- W2900580650 cites W2069694930 @default.
- W2900580650 cites W2072944279 @default.
- W2900580650 cites W2077474285 @default.
- W2900580650 cites W2079261882 @default.
- W2900580650 cites W2080016700 @default.
- W2900580650 cites W2080896529 @default.
- W2900580650 cites W2081315170 @default.
- W2900580650 cites W2082178644 @default.
- W2900580650 cites W2086018231 @default.
- W2900580650 cites W2092700187 @default.
- W2900580650 cites W2093832010 @default.
- W2900580650 cites W2093985321 @default.
- W2900580650 cites W2109558113 @default.
- W2900580650 cites W2116431176 @default.
- W2900580650 cites W2122137744 @default.
- W2900580650 cites W2124159149 @default.
- W2900580650 cites W2125353766 @default.
- W2900580650 cites W2129659516 @default.
- W2900580650 cites W2132956066 @default.
- W2900580650 cites W2134438519 @default.
- W2900580650 cites W2139519727 @default.
- W2900580650 cites W2141947008 @default.
- W2900580650 cites W2160951682 @default.
- W2900580650 cites W2166626013 @default.
- W2900580650 cites W2176713976 @default.
- W2900580650 cites W2236338088 @default.
- W2900580650 cites W2238068882 @default.
- W2900580650 cites W2267642330 @default.
- W2900580650 cites W2272798987 @default.
- W2900580650 cites W2286530017 @default.
- W2900580650 cites W2313394576 @default.
- W2900580650 cites W2317367263 @default.
- W2900580650 cites W2340194832 @default.
- W2900580650 cites W2341674473 @default.
- W2900580650 cites W2507195106 @default.
- W2900580650 cites W2512040808 @default.
- W2900580650 cites W2527589398 @default.
- W2900580650 cites W2573340532 @default.
- W2900580650 cites W2573682868 @default.
- W2900580650 cites W2581718004 @default.
- W2900580650 cites W2604412028 @default.