Matches in SemOpenAlex for { <https://semopenalex.org/work/W2900752015> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2900752015 endingPage "39" @default.
- W2900752015 startingPage "36" @default.
- W2900752015 abstract ": Objective: The aim of the present study was to screen the anticancer activity of Atractylis flava Desf in butanolic extract (AFBE). In addition, we attempted to investigate the gene expression levels of seven relevant genes involved in the pathways leading to toxicity: oxidative stress (NCF1, OPA1, SDHA), inflammation (TNFα), apoptotic balance (PDCD4, BCL2, CASP8) and the measurement of caspase-3 activity on the acute monocytic leukemia cell line THP-1. Material and Methods: The cell viability was assessed using Trypan blue exclusion, alamarBlue® and WST-1 assays. The gene expression levels were tested by RT-qPCR. Activity of caspase-3 activity was performed using the EnzChek® Caspase-3 Assay kit to confirm the induction of apoptosis on tumor cells. Results: We observed significant growth inhibitory activity of the AFBE extract on the acute monocytic leukemia cell line THP-1. Moreover, the gene expression analysis showed that the plant extract caused statistically significant downregulation of selected genes compared to the untreated cells. Caspase-3 activity in the treated cells was significantly elevated. Conclusion: The presents study states that the butanolic extract of Atractylis flava Desf showed a significant cytotoxic effect against THP-1 cells. Our results suggest that the AFBE may be beneficial for the treatment and prevention of cancer.Key words: Atractylis flava Desf, Gene expression, RT-qPCR, Apoptosis, THP-1." @default.
- W2900752015 created "2018-11-29" @default.
- W2900752015 creator A5000093793 @default.
- W2900752015 creator A5047180497 @default.
- W2900752015 creator A5048001501 @default.
- W2900752015 creator A5054568522 @default.
- W2900752015 creator A5079990942 @default.
- W2900752015 creator A5087148837 @default.
- W2900752015 date "2018-01-17" @default.
- W2900752015 modified "2023-09-24" @default.
- W2900752015 title "Gene Expression Levels of Selected Factors in Monocytic Leukemia Cell Line THP-1 Upon Treatment with n-butanol Extract of Atractylis flava Desf against Cancer" @default.
- W2900752015 doi "https://doi.org/10.5530/jyp.2019.11.8" @default.
- W2900752015 hasPublicationYear "2018" @default.
- W2900752015 type Work @default.
- W2900752015 sameAs 2900752015 @default.
- W2900752015 citedByCount "2" @default.
- W2900752015 countsByYear W29007520152021 @default.
- W2900752015 countsByYear W29007520152022 @default.
- W2900752015 crossrefType "journal-article" @default.
- W2900752015 hasAuthorship W2900752015A5000093793 @default.
- W2900752015 hasAuthorship W2900752015A5047180497 @default.
- W2900752015 hasAuthorship W2900752015A5048001501 @default.
- W2900752015 hasAuthorship W2900752015A5054568522 @default.
- W2900752015 hasAuthorship W2900752015A5079990942 @default.
- W2900752015 hasAuthorship W2900752015A5087148837 @default.
- W2900752015 hasBestOaLocation W29007520151 @default.
- W2900752015 hasConcept C104317684 @default.
- W2900752015 hasConcept C121608353 @default.
- W2900752015 hasConcept C150194340 @default.
- W2900752015 hasConcept C153911025 @default.
- W2900752015 hasConcept C190283241 @default.
- W2900752015 hasConcept C203014093 @default.
- W2900752015 hasConcept C204258353 @default.
- W2900752015 hasConcept C2776170302 @default.
- W2900752015 hasConcept C2778461978 @default.
- W2900752015 hasConcept C2780943621 @default.
- W2900752015 hasConcept C53227056 @default.
- W2900752015 hasConcept C54355233 @default.
- W2900752015 hasConcept C55493867 @default.
- W2900752015 hasConcept C556039675 @default.
- W2900752015 hasConcept C71924100 @default.
- W2900752015 hasConcept C81885089 @default.
- W2900752015 hasConcept C86803240 @default.
- W2900752015 hasConcept C96232424 @default.
- W2900752015 hasConcept C98274493 @default.
- W2900752015 hasConceptScore W2900752015C104317684 @default.
- W2900752015 hasConceptScore W2900752015C121608353 @default.
- W2900752015 hasConceptScore W2900752015C150194340 @default.
- W2900752015 hasConceptScore W2900752015C153911025 @default.
- W2900752015 hasConceptScore W2900752015C190283241 @default.
- W2900752015 hasConceptScore W2900752015C203014093 @default.
- W2900752015 hasConceptScore W2900752015C204258353 @default.
- W2900752015 hasConceptScore W2900752015C2776170302 @default.
- W2900752015 hasConceptScore W2900752015C2778461978 @default.
- W2900752015 hasConceptScore W2900752015C2780943621 @default.
- W2900752015 hasConceptScore W2900752015C53227056 @default.
- W2900752015 hasConceptScore W2900752015C54355233 @default.
- W2900752015 hasConceptScore W2900752015C55493867 @default.
- W2900752015 hasConceptScore W2900752015C556039675 @default.
- W2900752015 hasConceptScore W2900752015C71924100 @default.
- W2900752015 hasConceptScore W2900752015C81885089 @default.
- W2900752015 hasConceptScore W2900752015C86803240 @default.
- W2900752015 hasConceptScore W2900752015C96232424 @default.
- W2900752015 hasConceptScore W2900752015C98274493 @default.
- W2900752015 hasIssue "1" @default.
- W2900752015 hasLocation W29007520151 @default.
- W2900752015 hasOpenAccess W2900752015 @default.
- W2900752015 hasPrimaryLocation W29007520151 @default.
- W2900752015 hasRelatedWork W1566743293 @default.
- W2900752015 hasRelatedWork W2000736486 @default.
- W2900752015 hasRelatedWork W2029519920 @default.
- W2900752015 hasRelatedWork W2072166884 @default.
- W2900752015 hasRelatedWork W2127310510 @default.
- W2900752015 hasRelatedWork W2333401032 @default.
- W2900752015 hasRelatedWork W2364084648 @default.
- W2900752015 hasRelatedWork W2556565849 @default.
- W2900752015 hasRelatedWork W3001429828 @default.
- W2900752015 hasRelatedWork W4367362054 @default.
- W2900752015 hasVolume "11" @default.
- W2900752015 isParatext "false" @default.
- W2900752015 isRetracted "false" @default.
- W2900752015 magId "2900752015" @default.
- W2900752015 workType "article" @default.