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- W2900761477 abstract "The development of new bio-orthogonal ligation methods for the conjugation of native proteins is of particular importance in the field of chemical biology and biotherapies. In this work, we developed a traceless electrochemical method for protein bioconjugation. The electrochemically promoted tyrosine-click (e-Y-CLICK) allowed the chemoselective Y-modification of peptides and proteins with labeled urazoles. A low potential is applied in an electrochemical cell to activate urazole anchors in situ and on demand, without affecting the electroactive amino acids from the protein. The versatility of the electrosynthetic approach was shown on biologically relevant peptides and proteins such as oxytocin, angiotensin 2, serum bovine albumin, and epratuzumab. The fully conserved enzymatic activity of a glucose oxidase observed after e-Y-CLICK further highlights the softness of the method. The e-Y-CLICK protocols were successfully performed in pure aqueous buffers, without the need for co-solvents, scavenger or oxidizing chemicals, and should therefore significantly broaden the scope of bioconjugation." @default.
- W2900761477 created "2018-11-29" @default.
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- W2900761477 date "2018-11-13" @default.
- W2900761477 modified "2023-10-17" @default.
- W2900761477 title "Electrochemically Promoted Tyrosine-Click-Chemistry for Protein Labeling" @default.
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- W2900761477 doi "https://doi.org/10.1021/jacs.8b09372" @default.
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