FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2900772909> ?p ?o ?g. }

• W2900772909 abstract "Lachesis muta rhombeata (Lmr) is the largest venomous snake in Latin America and its venom contains mainly enzymatic components, such as serine and metalloproteases, L-amino acid oxidase and phospholipases A2. Metalloproteases comprise a large group of zinc-dependent proteases that cleave basement membrane components such as fibronectin, laminin and collagen type IV. These enzymes are responsible for local and systemic changes, including haemorrhage, myonecrosis and inflammation. This study aimed the isolation and enzymatic characterization of the first metalloprotease (Lmr-MP) from Lmr venom (LmrV).Lmr-MP was purified through two chromatographic steps and submitted to enzymatic characterization. It showed proteolytic activity on azocasein with maximum activity at pH 7.0-9.0. It was inhibited by EDTA (a metal chelator that removes zinc, which is essential for enzymatic activity) and no effect was observed with PMSF, iodoacetic acid or pepstatin (inhibitors of serine, cysteine and aspartyl proteases, respectively). Ca2+, Mg2+ and Ba2+ ions increased its activity, while Al3+, Cu2+, Ni2+ and Zn2+ inhibited it. Additionally, ZnCl2 showed a dose dependent inhibition of the enzyme. Lmr-MP activity was also evaluated upon chromogenic substrates for plasma kallikrein (S-2302), plasmin and streptokinase-activated plasminogen (S-2251) and Factor Xa (S-2222) showing the highest activity on S-2302. The activity in different solutions (5 mM or 50 mM ammonium bicarbonate, pH 7.8; 0.1% trifluoroacetic acid + 50% acetonitrile; phosphate buffer saline, pH 7.4; 50 mM sodium acetate, pH 4.0 or ammonium acetate pH 4.5) was also evaluated and the results showed that its activity was abolished at acidic pHs. Its molecular mass (22,858 Da) was determined by MALDI-TOF and about 90% of its primary structure was verified by high-resolution mass spectrometry using HCD and ETD fragmentations and database search against the sequence of closely related species. It is a novel enzyme which shared high identity with other snake venom metalloproteases (svMPs) belonging to the P-I group.The purification procedure achieved a novel pure highly active metalloprotease from LmrV. This new molecule can help to understand the metalloproteases mechanisms of action, the Lachesis envenoming, as well as to open new perspectives for its use as therapeutic tools." @default.
• W2900772909 created "2018-11-29" @default.
• W2900772909 creator A5022809598 @default.
• W2900772909 creator A5035840396 @default.
• W2900772909 creator A5036058013 @default.
• W2900772909 creator A5036689503 @default.
• W2900772909 creator A5045227946 @default.
• W2900772909 creator A5046586712 @default.
• W2900772909 creator A5058954001 @default.
• W2900772909 creator A5067341235 @default.
• W2900772909 creator A5078447854 @default.
• W2900772909 date "2018-11-22" @default.
• W2900772909 modified "2023-10-14" @default.
• W2900772909 title "Purification and enzymatic characterization of a novel metalloprotease from Lachesis muta rhombeata snake venom" @default.
• W2900772909 cites W1259707545 @default.
• W2900772909 cites W1565563859 @default.
• W2900772909 cites W1879340618 @default.
• W2900772909 cites W1966870640 @default.
• W2900772909 cites W1976083674 @default.
• W2900772909 cites W1977229831 @default.
• W2900772909 cites W1977533398 @default.
• W2900772909 cites W1978771975 @default.
• W2900772909 cites W1979816412 @default.
• W2900772909 cites W1980719058 @default.
• W2900772909 cites W1981639053 @default.
• W2900772909 cites W1992304324 @default.
• W2900772909 cites W2014209916 @default.
• W2900772909 cites W2018289835 @default.
• W2900772909 cites W2024523907 @default.
• W2900772909 cites W2027754536 @default.
• W2900772909 cites W2053235962 @default.
• W2900772909 cites W2054721875 @default.
• W2900772909 cites W2068329737 @default.
• W2900772909 cites W2078420786 @default.
• W2900772909 cites W2080278469 @default.
• W2900772909 cites W2081547052 @default.
• W2900772909 cites W2082192485 @default.
• W2900772909 cites W2100837269 @default.
• W2900772909 cites W2101124187 @default.
• W2900772909 cites W2107198382 @default.
• W2900772909 cites W2108172578 @default.
• W2900772909 cites W2110438218 @default.
• W2900772909 cites W2123468098 @default.
• W2900772909 cites W2161900366 @default.
• W2900772909 cites W2172041325 @default.
• W2900772909 cites W2281089040 @default.
• W2900772909 cites W2295027400 @default.
• W2900772909 cites W2416985615 @default.
• W2900772909 cites W2527520038 @default.
• W2900772909 cites W2529278636 @default.
• W2900772909 cites W2535803710 @default.
• W2900772909 cites W2576357047 @default.
• W2900772909 cites W2606594229 @default.
• W2900772909 cites W2775035200 @default.
• W2900772909 cites W2792624593 @default.
• W2900772909 cites W4244736178 @default.
• W2900772909 doi "https://doi.org/10.1186/s40409-018-0171-x" @default.
• W2900772909 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6251203" @default.
• W2900772909 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30498508" @default.
• W2900772909 hasPublicationYear "2018" @default.
• W2900772909 type Work @default.
• W2900772909 sameAs 2900772909 @default.
• W2900772909 citedByCount "11" @default.
• W2900772909 countsByYear W29007729092020 @default.
• W2900772909 countsByYear W29007729092021 @default.
• W2900772909 countsByYear W29007729092022 @default.
• W2900772909 countsByYear W29007729092023 @default.
• W2900772909 crossrefType "journal-article" @default.
• W2900772909 hasAuthorship W2900772909A5022809598 @default.
• W2900772909 hasAuthorship W2900772909A5035840396 @default.
• W2900772909 hasAuthorship W2900772909A5036058013 @default.
• W2900772909 hasAuthorship W2900772909A5036689503 @default.
• W2900772909 hasAuthorship W2900772909A5045227946 @default.
• W2900772909 hasAuthorship W2900772909A5046586712 @default.
• W2900772909 hasAuthorship W2900772909A5058954001 @default.
• W2900772909 hasAuthorship W2900772909A5067341235 @default.
• W2900772909 hasAuthorship W2900772909A5078447854 @default.
• W2900772909 hasBestOaLocation W29007729091 @default.
• W2900772909 hasConcept C178790620 @default.
• W2900772909 hasConcept C181199279 @default.
• W2900772909 hasConcept C182220744 @default.
• W2900772909 hasConcept C185020521 @default.
• W2900772909 hasConcept C185592680 @default.
• W2900772909 hasConcept C197404232 @default.
• W2900772909 hasConcept C2776350231 @default.
• W2900772909 hasConcept C2776714187 @default.
• W2900772909 hasConcept C2776725428 @default.
• W2900772909 hasConcept C2777807008 @default.
• W2900772909 hasConcept C2778973965 @default.
• W2900772909 hasConcept C2779448229 @default.
• W2900772909 hasConcept C2780197888 @default.
• W2900772909 hasConcept C2780340462 @default.
• W2900772909 hasConcept C2780865716 @default.
• W2900772909 hasConcept C55493867 @default.
• W2900772909 hasConcept C55728118 @default.
• W2900772909 hasConceptScore W2900772909C178790620 @default.
• W2900772909 hasConceptScore W2900772909C181199279 @default.
• W2900772909 hasConceptScore W2900772909C182220744 @default.
• W2900772909 hasConceptScore W2900772909C185020521 @default.
• W2900772909 hasConceptScore W2900772909C185592680 @default.

• next