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• W2900820312 abstract "Green-to-red photoconvertible fluorescent proteins repeatedly enter dark states, causing interrupted tracks in single-particle-tracking localization microscopy (sptPALM). We identified a long-lived dark state in photoconverted mEos4b that results from isomerization of the chromophore and efficiently absorbs cyan light. Addition of weak 488-nm light swiftly reverts this dark state to the fluorescent state. This strategy largely eliminates slow blinking and enables the recording of significantly longer tracks in sptPALM with minimum effort." @default.
• W2900820312 created "2018-11-29" @default.
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• W2900820312 date "2018-11-21" @default.
• W2900820312 modified "2023-09-25" @default.
• W2900820312 title "Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM" @default.
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• W2900820312 doi "https://doi.org/10.1101/475939" @default.
• W2900820312 hasPublicationYear "2018" @default.
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