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- W2901353670 abstract "5144 Real-time PCR as a powerful and versatile tool has been widely used in R&D and other practical fields. One-step RT-PCR combines reverse transcription of RNA to cDNA with PCR amplification into a single step operation, and it offers fast turn-around time of results to researchers. Using dsDNA binding dye SYBR Green I as a reporter, with no FRET probe required, SYBR Green PCR has become one of the main chemistries in real-time PCR because it is easy to use, flexible, and inexpensive. However, PCR not only can amplify wanted DNA target but also can amplify non-targeted DNA or generate primer-dimers under certain conditions. Because of this, sometimes the results become ambiguous, especially for SYBR 1-step RT-PCR since SYBR Green can detect any double strand DNA. To overcome this problem, we have developed a new SYBR one-step RT-PCR master mix kit: Power SYBR Green RNA-To-C T TM 1-Step Kit. This pre-formulated kit contains all of the reagents necessary for performing SYBR Green one-step RT-PCR, including our new high purity hot-start AmpliTaq Gold ® DNA Polymerase UP (Ultra Pure) and robust reverse transcriptase, ArrayScript. Extensive testing has shown that this kit can deliver highly sensitive and specific quantitation over a wide range of RNA template concentration, detecting as low as a single copy of target gene." @default.
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- W2901353670 date "2008-05-01" @default.
- W2901353670 modified "2023-09-26" @default.
- W2901353670 title "A powerful sybr® green one-step rt-pcr master mix tool: Power sybr green RNA-to-CTtm 1-step kit" @default.
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