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- W2901368419 abstract "Ganglioside content in cultured dairy products is significant and may contribute to antimicrobial effects associated with such foods (J Food Comp Anal13: 783, 2000). The source of gangliosides is unclear as dairy products are generated from ganglioside-free components (skim milk and culture bacteria). By a terminal synthetic reaction, sialyltransferase (ST) transfers N-acetylneuraminic acid (NANA) to lactosylceramide (LC): CMP-NANA + HO- lactosylceramide → NANA-lactosylceramide + CMP. Formation of gangliosides (eg NANA-LC) is visualized by thin-layer chromatography (TLC) via resorcinol staining. Incubation conditions have been optimized. Analysis with multiple NANA acceptors (LC solution, whey, dialyzed yogurt, and milk) indicates that milk contains an appropriate acceptor, yielding a NANA containing product (putative ganglioside) with an Rf (0.71) that is distinct from the Rf (0.63) of free NANA. Now that it has been determined that appropriate NANA acceptors are inherent in milk, the presence of ST in dairy components can be assessed. Rich ganglioside content may be a synergistic by-product among dairy components. (Supported by OU Provost's Undergraduate Research Award)" @default.
- W2901368419 created "2018-11-29" @default.
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- W2901368419 date "2013-04-01" @default.
- W2901368419 modified "2023-09-26" @default.
- W2901368419 title "TLC‐based assay of ganglioside synthesis" @default.
- W2901368419 doi "https://doi.org/10.1096/fasebj.27.1_supplement.800.1" @default.
- W2901368419 hasPublicationYear "2013" @default.
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