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• W2901409616 abstract "Pseudorabies virus (PRV) is a pathogen that causes an acute infectious disease in pigs, which could lead to huge losses to the farming industry. The Bartha-K61 strain of PRV, commonly used as a gE-deleted vaccine, does not always protect against the wild-type virus infection effectively. Therefore, the prompt detection of viral infection in gE-deleted vaccine vaccinated pigs is crucial for in-time measures to prevent the spread of diseases. In this study, we developed a Surface-enhanced Raman spectroscopy(SERS) based lateral flow assay based on antigen-antibody reaction to meet the demand. Our method was rapid (15 min), sensitive (LOD: 5 ng mL−1), selective for wild-type PRV detection, and quantitatively or semi-quantitatively (DLR: 41–650 ng mL−1) compatible. The detection results from this method were consistent with results from the gE-specific PCR, indicating that this SERS-based lateral flow assay could be used as a new tool to differentially diagnose wild-type PRV and gE-deleted vaccine." @default.
• W2901409616 created "2018-11-29" @default.
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• W2901409616 date "2019-03-01" @default.
• W2901409616 modified "2023-10-02" @default.
• W2901409616 title "A novel SERS-based lateral flow assay for differential diagnosis of wild-type pseudorabies virus and gE-deleted vaccine" @default.
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• W2901409616 doi "https://doi.org/10.1016/j.snb.2018.11.065" @default.
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