Matches in SemOpenAlex for { <https://semopenalex.org/work/W2901428946> ?p ?o ?g. }
- W2901428946 endingPage "155" @default.
- W2901428946 startingPage "149" @default.
- W2901428946 abstract "Immobilization of β–galactosidase (β-gal) into porous materials might afford to supported biocatalysts for the hydrolysis of diary products or to food additives for lactose intolerant people. Activity and stability of the loaded material generally depend on the interactions between the enzyme and the support, as well as on the pore size. Herein, Raman spectroscopy was used to evaluate the specific adsorption the enzyme into meso-macroporous silica materials, containing interconnected mesopores of 9 nm and macropores of 200 nm. Non-porous silica was used as reference material to determine the Raman fingerprint of physisorbed enzyme in the absence of any confinement. While the β-gal physisorbed on the surface of non-porous silica material exhibits the same Raman spectra as the free enzyme, the enzyme physisorbed onto meso-macroporous materials show frequency displacements of characteristic amide groups as a function of initial concentration of the feed enzymatic solution. In fact, at low initial concentration in enzyme, no shifts of the amides were recorded on Raman spectra as compared with free enzyme, indicating a preferential physisorption into macropores. By increasing the enzyme concentration, the frequency of Amide I was shifted to lower values, suggesting thus a confinement into mesopores. Finally, the enzyme concentration effect can be demonstrated by the increment of the amide band intensity in the range of 1700-1500 cm−1 as the amount of adsorbed enzyme increases. Thus, the textural properties of silica materials seem to be the key factor in the enzyme adsorption." @default.
- W2901428946 created "2018-11-29" @default.
- W2901428946 creator A5013901887 @default.
- W2901428946 creator A5017751610 @default.
- W2901428946 creator A5048431419 @default.
- W2901428946 creator A5058838850 @default.
- W2901428946 creator A5077513897 @default.
- W2901428946 creator A5085463554 @default.
- W2901428946 date "2019-04-01" @default.
- W2901428946 modified "2023-10-18" @default.
- W2901428946 title "Probing the confinement of β-galactosidase into meso-macro porous silica by Raman spectroscopy" @default.
- W2901428946 cites W1530729903 @default.
- W2901428946 cites W1763950462 @default.
- W2901428946 cites W1974243561 @default.
- W2901428946 cites W1982350871 @default.
- W2901428946 cites W1984128566 @default.
- W2901428946 cites W1988622418 @default.
- W2901428946 cites W1993642618 @default.
- W2901428946 cites W1995862771 @default.
- W2901428946 cites W2003304269 @default.
- W2901428946 cites W2003327200 @default.
- W2901428946 cites W2020730968 @default.
- W2901428946 cites W2034035344 @default.
- W2901428946 cites W2042372239 @default.
- W2901428946 cites W2055499711 @default.
- W2901428946 cites W2067943844 @default.
- W2901428946 cites W2069969637 @default.
- W2901428946 cites W2073641595 @default.
- W2901428946 cites W2109646052 @default.
- W2901428946 cites W2126077839 @default.
- W2901428946 cites W2136772655 @default.
- W2901428946 cites W2151551785 @default.
- W2901428946 cites W2159426997 @default.
- W2901428946 cites W2257759118 @default.
- W2901428946 cites W2318475041 @default.
- W2901428946 cites W2407591075 @default.
- W2901428946 cites W2494565466 @default.
- W2901428946 cites W2549081437 @default.
- W2901428946 cites W2597150075 @default.
- W2901428946 cites W2614047159 @default.
- W2901428946 cites W2621502726 @default.
- W2901428946 cites W2746871434 @default.
- W2901428946 cites W2793556711 @default.
- W2901428946 cites W2794044952 @default.
- W2901428946 cites W2887259874 @default.
- W2901428946 doi "https://doi.org/10.1016/j.micromeso.2018.11.032" @default.
- W2901428946 hasPublicationYear "2019" @default.
- W2901428946 type Work @default.
- W2901428946 sameAs 2901428946 @default.
- W2901428946 citedByCount "7" @default.
- W2901428946 countsByYear W29014289462019 @default.
- W2901428946 countsByYear W29014289462020 @default.
- W2901428946 countsByYear W29014289462021 @default.
- W2901428946 countsByYear W29014289462023 @default.
- W2901428946 crossrefType "journal-article" @default.
- W2901428946 hasAuthorship W2901428946A5013901887 @default.
- W2901428946 hasAuthorship W2901428946A5017751610 @default.
- W2901428946 hasAuthorship W2901428946A5048431419 @default.
- W2901428946 hasAuthorship W2901428946A5058838850 @default.
- W2901428946 hasAuthorship W2901428946A5077513897 @default.
- W2901428946 hasAuthorship W2901428946A5085463554 @default.
- W2901428946 hasConcept C113196181 @default.
- W2901428946 hasConcept C120665830 @default.
- W2901428946 hasConcept C121332964 @default.
- W2901428946 hasConcept C127413603 @default.
- W2901428946 hasConcept C150394285 @default.
- W2901428946 hasConcept C161790260 @default.
- W2901428946 hasConcept C178790620 @default.
- W2901428946 hasConcept C181199279 @default.
- W2901428946 hasConcept C185592680 @default.
- W2901428946 hasConcept C192562407 @default.
- W2901428946 hasConcept C2777496208 @default.
- W2901428946 hasConcept C2780050857 @default.
- W2901428946 hasConcept C33633552 @default.
- W2901428946 hasConcept C40003534 @default.
- W2901428946 hasConcept C42360764 @default.
- W2901428946 hasConcept C43617362 @default.
- W2901428946 hasConcept C6648577 @default.
- W2901428946 hasConcept C82776694 @default.
- W2901428946 hasConceptScore W2901428946C113196181 @default.
- W2901428946 hasConceptScore W2901428946C120665830 @default.
- W2901428946 hasConceptScore W2901428946C121332964 @default.
- W2901428946 hasConceptScore W2901428946C127413603 @default.
- W2901428946 hasConceptScore W2901428946C150394285 @default.
- W2901428946 hasConceptScore W2901428946C161790260 @default.
- W2901428946 hasConceptScore W2901428946C178790620 @default.
- W2901428946 hasConceptScore W2901428946C181199279 @default.
- W2901428946 hasConceptScore W2901428946C185592680 @default.
- W2901428946 hasConceptScore W2901428946C192562407 @default.
- W2901428946 hasConceptScore W2901428946C2777496208 @default.
- W2901428946 hasConceptScore W2901428946C2780050857 @default.
- W2901428946 hasConceptScore W2901428946C33633552 @default.
- W2901428946 hasConceptScore W2901428946C40003534 @default.
- W2901428946 hasConceptScore W2901428946C42360764 @default.
- W2901428946 hasConceptScore W2901428946C43617362 @default.
- W2901428946 hasConceptScore W2901428946C6648577 @default.
- W2901428946 hasConceptScore W2901428946C82776694 @default.
- W2901428946 hasFunder F4320333065 @default.