Matches in SemOpenAlex for { <https://semopenalex.org/work/W2901552404> ?p ?o ?g. }
- W2901552404 abstract "Abstract Although polymerase chain reaction (PCR) is the most widely used method for DNA amplification, the requirement of thermocycling limits its non-laboratory applications. Isothermal DNA amplification techniques are hence valuable for on-site diagnostic applications in place of traditional PCR. Here we describe a true isothermal approach for amplifying and detecting double-stranded DNA based on a CRISPR–Cas9-triggered nicking endonuclease-mediated Strand Displacement Amplification method (namely CRISDA). CRISDA takes advantage of the high sensitivity/specificity and unique conformational rearrangements of CRISPR effectors in recognizing the target DNA. In combination with a peptide nucleic acid (PNA) invasion-mediated endpoint measurement, the method exhibits attomolar sensitivity and single-nucleotide specificity in detection of various DNA targets under a complex sample background. Additionally, by integrating the technique with a Cas9-mediated target enrichment approach, CRISDA exhibits sub-attomolar sensitivity. In summary, CRISDA is a powerful isothermal tool for ultrasensitive and specific detection of nucleic acids in point-of-care diagnostics and field analyses." @default.
- W2901552404 created "2018-11-29" @default.
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- W2901552404 date "2018-11-27" @default.
- W2901552404 modified "2023-10-03" @default.
- W2901552404 title "A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection" @default.
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- W2901552404 doi "https://doi.org/10.1038/s41467-018-07324-5" @default.
- W2901552404 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6258682" @default.
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