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• W2901664219 endingPage "678" @default.
• W2901664219 startingPage "662" @default.
• W2901664219 abstract "Pseudomonas aeruginosa (Pa) expresses an adhesin, flagellin, that engages the mucin 1 (MUC1) ectodomain (ED) expressed on airway epithelia, increasing association of MUC1-ED with neuraminidase 1 (NEU1) and MUC1-ED desialylation. The MUC1-ED desialylation unmasks both cryptic binding sites for Pa and a protease recognition site, permitting its proteolytic release as a hyperadhesive decoy receptor for Pa. We found here that intranasal administration of Pa strain K (PAK) to BALB/c mice increases MUC1-ED shedding into the bronchoalveolar compartment. MUC1-ED levels increased as early as 12 h, peaked at 24–48 h with a 7.8-fold increase, and decreased by 72 h. The a-type flagellin–expressing PAK strain and the b-type flagellin–expressing PAO1 strain stimulated comparable levels of MUC1-ED shedding. A flagellin-deficient PAK mutant provoked dramatically reduced MUC1-ED shedding compared with the WT strain, and purified flagellin recapitulated the WT effect. In lung tissues, Pa increased association of NEU1 and protective protein/cathepsin A with MUC1-ED in reciprocal co-immunoprecipitation assays and stimulated MUC1-ED desialylation. NEU1-selective sialidase inhibition protected against Pa-induced MUC1-ED desialylation and shedding. In Pa-challenged mice, MUC1-ED–enriched bronchoalveolar lavage fluid (BALF) inhibited flagellin binding and Pa adhesion to human airway epithelia by up to 44% and flagellin-driven motility by >30%. Finally, Pa co-administration with recombinant human MUC1-ED dramatically diminished lung and BALF bacterial burden, proinflammatory cytokine levels, and pulmonary leukostasis and increased 5-day survival from 0% to 75%. We conclude that Pa flagellin provokes NEU1-mediated airway shedding of MUC1-ED, which functions as a decoy receptor protecting against lethal Pa lung infection. Pseudomonas aeruginosa (Pa) expresses an adhesin, flagellin, that engages the mucin 1 (MUC1) ectodomain (ED) expressed on airway epithelia, increasing association of MUC1-ED with neuraminidase 1 (NEU1) and MUC1-ED desialylation. The MUC1-ED desialylation unmasks both cryptic binding sites for Pa and a protease recognition site, permitting its proteolytic release as a hyperadhesive decoy receptor for Pa. We found here that intranasal administration of Pa strain K (PAK) to BALB/c mice increases MUC1-ED shedding into the bronchoalveolar compartment. MUC1-ED levels increased as early as 12 h, peaked at 24–48 h with a 7.8-fold increase, and decreased by 72 h. The a-type flagellin–expressing PAK strain and the b-type flagellin–expressing PAO1 strain stimulated comparable levels of MUC1-ED shedding. A flagellin-deficient PAK mutant provoked dramatically reduced MUC1-ED shedding compared with the WT strain, and purified flagellin recapitulated the WT effect. In lung tissues, Pa increased association of NEU1 and protective protein/cathepsin A with MUC1-ED in reciprocal co-immunoprecipitation assays and stimulated MUC1-ED desialylation. NEU1-selective sialidase inhibition protected against Pa-induced MUC1-ED desialylation and shedding. In Pa-challenged mice, MUC1-ED–enriched bronchoalveolar lavage fluid (BALF) inhibited flagellin binding and Pa adhesion to human airway epithelia by up to 44% and flagellin-driven motility by >30%. Finally, Pa co-administration with recombinant human MUC1-ED dramatically diminished lung and BALF bacterial burden, proinflammatory cytokine levels, and pulmonary leukostasis and increased 5-day survival from 0% to 75%. We conclude that Pa flagellin provokes NEU1-mediated airway shedding of MUC1-ED, which functions as a decoy receptor protecting against lethal Pa lung infection." @default.
• W2901664219 created "2018-11-29" @default.
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• W2901664219 date "2019-01-01" @default.
• W2901664219 modified "2023-10-06" @default.
• W2901664219 title "Neuraminidase 1–mediated desialylation of the mucin 1 ectodomain releases a decoy receptor that protects against Pseudomonas aeruginosa lung infection" @default.
• W2901664219 cites W111579719 @default.
• W2901664219 cites W1504831414 @default.
• W2901664219 cites W1556549064 @default.
• W2901664219 cites W1585599668 @default.
• W2901664219 cites W1869871526 @default.
• W2901664219 cites W1886517296 @default.
• W2901664219 cites W1927110920 @default.
• W2901664219 cites W1940030194 @default.
• W2901664219 cites W1957962135 @default.
• W2901664219 cites W1959914164 @default.
• W2901664219 cites W1966365711 @default.
• W2901664219 cites W1968259615 @default.
• W2901664219 cites W1970087902 @default.
• W2901664219 cites W1982843262 @default.
• W2901664219 cites W1988201936 @default.
• W2901664219 cites W1995217818 @default.
• W2901664219 cites W1997991343 @default.
• W2901664219 cites W2004801337 @default.
• W2901664219 cites W2007121859 @default.
• W2901664219 cites W2008207679 @default.
• W2901664219 cites W2008343864 @default.
• W2901664219 cites W2010571201 @default.
• W2901664219 cites W2013343134 @default.
• W2901664219 cites W2013359703 @default.
• W2901664219 cites W2014594219 @default.
• W2901664219 cites W2031779604 @default.
• W2901664219 cites W2032293954 @default.
• W2901664219 cites W2034179694 @default.
• W2901664219 cites W2041274762 @default.
• W2901664219 cites W2042722056 @default.
• W2901664219 cites W2044086315 @default.
• W2901664219 cites W2047317128 @default.
• W2901664219 cites W2052318993 @default.
• W2901664219 cites W2062503200 @default.
• W2901664219 cites W2067634655 @default.
• W2901664219 cites W2072753520 @default.
• W2901664219 cites W2076370383 @default.
• W2901664219 cites W2076838043 @default.
• W2901664219 cites W2078410720 @default.
• W2901664219 cites W2079273426 @default.
• W2901664219 cites W2085436045 @default.
• W2901664219 cites W2088730218 @default.
• W2901664219 cites W2093403176 @default.
• W2901664219 cites W2095293817 @default.
• W2901664219 cites W2096684565 @default.
• W2901664219 cites W2098483342 @default.
• W2901664219 cites W2100343582 @default.
• W2901664219 cites W2101591039 @default.
• W2901664219 cites W2109068081 @default.
• W2901664219 cites W2114640915 @default.
• W2901664219 cites W2115572803 @default.
• W2901664219 cites W2121080125 @default.
• W2901664219 cites W2121373279 @default.
• W2901664219 cites W2126648631 @default.
• W2901664219 cites W2128480720 @default.
• W2901664219 cites W2136052505 @default.
• W2901664219 cites W2137003603 @default.
• W2901664219 cites W2140611106 @default.
• W2901664219 cites W2142153279 @default.
• W2901664219 cites W2148000616 @default.
• W2901664219 cites W2148235564 @default.
• W2901664219 cites W2149353640 @default.
• W2901664219 cites W2150173080 @default.
• W2901664219 cites W2151157551 @default.
• W2901664219 cites W2154492506 @default.
• W2901664219 cites W2159830267 @default.
• W2901664219 cites W2161440756 @default.
• W2901664219 cites W2165589681 @default.
• W2901664219 cites W2184505783 @default.
• W2901664219 cites W2259315983 @default.
• W2901664219 cites W2298741050 @default.
• W2901664219 cites W2405781470 @default.
• W2901664219 cites W2560740268 @default.
• W2901664219 cites W2889991839 @default.
• W2901664219 cites W2993790425 @default.
• W2901664219 doi "https://doi.org/10.1074/jbc.ra118.006022" @default.
• W2901664219 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6333888" @default.
• W2901664219 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30429216" @default.
• W2901664219 hasPublicationYear "2019" @default.
• W2901664219 type Work @default.
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• W2901664219 citedByCount "14" @default.

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