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- W2901674702 abstract "Abstract Many bacterial autotrophs rely on CO 2 concentrating mechanisms (CCMs) to assimilate carbon. Although many CCM proteins have been identified, including a 200+ MDa protein organelle called the carboxysome, a systematic screen of CCM components has not been carried out. Here, we performed a genome-wide barcoded transposon screen to identify essential and CCM-related genes in the ɣ-proteobacterium H. neapolitanus . Our screen revealed an operon critical for CCM function which encodes a domain of unknown function (PFAM:PF10070) and putative cation transporter subunit (PFAM:PF00361). These two proteins, which we name DabA and DabB for “DABs accumulate bicarbonate,” function as a heterodimeric, energy-coupled inorganic carbon pump in E. coli . Furthermore, DabA binds zinc and has a an active site homologous to a β-carbonic anhydrase. Based on these results, we propose that DABs function as vectorial CAs coupled to cation gradients and serve as inorganic carbon pumps throughout prokaryotic phyla." @default.
- W2901674702 created "2018-11-29" @default.
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- W2901674702 date "2018-11-22" @default.
- W2901674702 modified "2023-10-14" @default.
- W2901674702 title "Genome-wide screening reveals a novel class of carbonic anhydrase-like inorganic carbon pumps in chemoautotrophic bacteria" @default.
- W2901674702 cites W12673073 @default.
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- W2901674702 cites W1587606930 @default.
- W2901674702 cites W1651951397 @default.
- W2901674702 cites W1803102843 @default.
- W2901674702 cites W1869640752 @default.
- W2901674702 cites W1898780412 @default.
- W2901674702 cites W1964537087 @default.
- W2901674702 cites W1968546335 @default.
- W2901674702 cites W1970843571 @default.
- W2901674702 cites W1975884928 @default.
- W2901674702 cites W1977237612 @default.
- W2901674702 cites W1979626519 @default.
- W2901674702 cites W1991512591 @default.
- W2901674702 cites W1994314761 @default.
- W2901674702 cites W1995403970 @default.
- W2901674702 cites W1997387703 @default.
- W2901674702 cites W2014598704 @default.
- W2901674702 cites W2018267605 @default.
- W2901674702 cites W2021770354 @default.
- W2901674702 cites W2027039022 @default.
- W2901674702 cites W2032760653 @default.
- W2901674702 cites W2034586585 @default.
- W2901674702 cites W2037312364 @default.
- W2901674702 cites W2043122290 @default.
- W2901674702 cites W2047178318 @default.
- W2901674702 cites W2053271424 @default.
- W2901674702 cites W2057327357 @default.
- W2901674702 cites W2057646661 @default.
- W2901674702 cites W2058906765 @default.
- W2901674702 cites W2066485538 @default.
- W2901674702 cites W2070722760 @default.
- W2901674702 cites W2077130210 @default.
- W2901674702 cites W2081242643 @default.
- W2901674702 cites W2089351638 @default.
- W2901674702 cites W2093181933 @default.
- W2901674702 cites W2108192908 @default.
- W2901674702 cites W2108651382 @default.
- W2901674702 cites W2110751375 @default.
- W2901674702 cites W2111967267 @default.
- W2901674702 cites W2112164915 @default.
- W2901674702 cites W2124019798 @default.
- W2901674702 cites W2124951680 @default.
- W2901674702 cites W2134505057 @default.
- W2901674702 cites W2135378361 @default.
- W2901674702 cites W2136700678 @default.
- W2901674702 cites W2138289353 @default.
- W2901674702 cites W2138695863 @default.
- W2901674702 cites W2140756273 @default.
- W2901674702 cites W2140894037 @default.
- W2901674702 cites W2148641266 @default.
- W2901674702 cites W2149717752 @default.
- W2901674702 cites W2151545731 @default.
- W2901674702 cites W2161146088 @default.
- W2901674702 cites W2162093645 @default.
- W2901674702 cites W2165259257 @default.
- W2901674702 cites W2165335121 @default.
- W2901674702 cites W2168521460 @default.
- W2901674702 cites W2169515696 @default.
- W2901674702 cites W2286023125 @default.
- W2901674702 cites W2296883552 @default.
- W2901674702 cites W2335924208 @default.
- W2901674702 cites W2336330109 @default.
- W2901674702 cites W2366572993 @default.
- W2901674702 cites W2468352349 @default.
- W2901674702 cites W2471456962 @default.
- W2901674702 cites W2508450075 @default.
- W2901674702 cites W2582820778 @default.
- W2901674702 cites W2612178113 @default.
- W2901674702 cites W2613813743 @default.
- W2901674702 cites W2614475007 @default.
- W2901674702 cites W2750791471 @default.
- W2901674702 cites W2775683081 @default.
- W2901674702 cites W2792176600 @default.
- W2901674702 cites W2793818469 @default.
- W2901674702 cites W2805022299 @default.
- W2901674702 cites W2810059792 @default.
- W2901674702 cites W2889380634 @default.
- W2901674702 cites W2900516269 @default.
- W2901674702 cites W3838968 @default.
- W2901674702 doi "https://doi.org/10.1101/476713" @default.
- W2901674702 hasPublicationYear "2018" @default.
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