FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2901681617> ?p ?o ?g. }

• W2901681617 abstract "MRTF-A (myocardin-related transcription factor A) is a coactivator for SRF-mediated gene expression. The activity of MRTF-A is critically dependent on the dissociation of G-actin from N-terminal RPEL motifs. MRTF-SRF induction often correlates with enhanced polymerization of F-actin. Here we investigate MRTF regulation by the multifunctional JMY protein, which contains three WASP/verprolin homology 2 (WH2/V) domains and facilitates Arp2/3-dependent and -independent actin nucleation.Co-immunoprecipitation experiments, immunofluorescence and luciferase reporter assays were combined with selective inhibitors to investigate the effect of JMY and its domains on MRTF-A in NIH 3 T3 mouse fibroblasts.JMY induced MRTF-A transcriptional activity and enhanced its nuclear translocation. Unexpectedly, MRTF-A was hyperactivated when the Arp2/3-recruiting CA region of JMY was deleted or mutated, suggesting an autoinhibitory mechanism for full-length JMY. Moreover, isolated WH2/V domains which are unable to nucleate actin were sufficient for nuclear accumulation and SRF activation. Recombinant WH2/V regions of JMY biochemically competed with MRTF-A for actin binding. Activation of MRTF-A by JMY was unaffected by Arp3 knockdown, by an Arp2/3 inhibitor, and by latrunculin which disassembles cellular F-actin. Restriction of JMY to the nucleus abrogated its MRTF-A activation. Finally, JMY RNAi reduced basal and stimulated transcriptional activation via MRTF-A.Our results suggest that JMY activates MRTF-SRF independently of F-actin via WH2/V-mediated competition with the RPEL region for G-actin binding in the cytoplasm. Furthermore, the C-terminal region facilitates an autoinhibitory effect on full-length JMY, possibly by intramolecular folding." @default.
• W2901681617 created "2018-11-29" @default.
• W2901681617 creator A5008004580 @default.
• W2901681617 creator A5011132701 @default.
• W2901681617 creator A5033023125 @default.
• W2901681617 creator A5066456827 @default.
• W2901681617 creator A5071437248 @default.
• W2901681617 date "2018-11-21" @default.
• W2901681617 modified "2023-10-18" @default.
• W2901681617 title "Regulation of MRTF-A by JMY via a nucleation-independent mechanism" @default.
• W2901681617 cites W1835930021 @default.
• W2901681617 cites W1962519351 @default.
• W2901681617 cites W1967487834 @default.
• W2901681617 cites W1968342621 @default.
• W2901681617 cites W1974088360 @default.
• W2901681617 cites W1978228904 @default.
• W2901681617 cites W1984718084 @default.
• W2901681617 cites W1993810735 @default.
• W2901681617 cites W1999708992 @default.
• W2901681617 cites W2009191441 @default.
• W2901681617 cites W2023687572 @default.
• W2901681617 cites W2025275840 @default.
• W2901681617 cites W2026993081 @default.
• W2901681617 cites W2031730524 @default.
• W2901681617 cites W2039059036 @default.
• W2901681617 cites W2046954672 @default.
• W2901681617 cites W2059161447 @default.
• W2901681617 cites W2083702734 @default.
• W2901681617 cites W2085616957 @default.
• W2901681617 cites W2090392605 @default.
• W2901681617 cites W2093723806 @default.
• W2901681617 cites W2103428229 @default.
• W2901681617 cites W2104909766 @default.
• W2901681617 cites W2107625881 @default.
• W2901681617 cites W2107882112 @default.
• W2901681617 cites W2123110782 @default.
• W2901681617 cites W2126759033 @default.
• W2901681617 cites W2131200617 @default.
• W2901681617 cites W2143790852 @default.
• W2901681617 cites W2314513070 @default.
• W2901681617 cites W2346403837 @default.
• W2901681617 cites W2598084305 @default.
• W2901681617 cites W2663610293 @default.
• W2901681617 cites W2764157960 @default.
• W2901681617 cites W939692825 @default.
• W2901681617 doi "https://doi.org/10.1186/s12964-018-0299-x" @default.
• W2901681617 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6249979" @default.
• W2901681617 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30463620" @default.
• W2901681617 hasPublicationYear "2018" @default.
• W2901681617 type Work @default.
• W2901681617 sameAs 2901681617 @default.
• W2901681617 citedByCount "2" @default.
• W2901681617 countsByYear W29016816172021 @default.
• W2901681617 countsByYear W29016816172022 @default.
• W2901681617 crossrefType "journal-article" @default.
• W2901681617 hasAuthorship W2901681617A5008004580 @default.
• W2901681617 hasAuthorship W2901681617A5011132701 @default.
• W2901681617 hasAuthorship W2901681617A5033023125 @default.
• W2901681617 hasAuthorship W2901681617A5066456827 @default.
• W2901681617 hasAuthorship W2901681617A5071437248 @default.
• W2901681617 hasBestOaLocation W29016816171 @default.
• W2901681617 hasConcept C104317684 @default.
• W2901681617 hasConcept C125705527 @default.
• W2901681617 hasConcept C190062978 @default.
• W2901681617 hasConcept C54355233 @default.
• W2901681617 hasConcept C55493867 @default.
• W2901681617 hasConcept C71829478 @default.
• W2901681617 hasConcept C81885089 @default.
• W2901681617 hasConcept C86339819 @default.
• W2901681617 hasConcept C86803240 @default.
• W2901681617 hasConcept C95444343 @default.
• W2901681617 hasConceptScore W2901681617C104317684 @default.
• W2901681617 hasConceptScore W2901681617C125705527 @default.
• W2901681617 hasConceptScore W2901681617C190062978 @default.
• W2901681617 hasConceptScore W2901681617C54355233 @default.
• W2901681617 hasConceptScore W2901681617C55493867 @default.
• W2901681617 hasConceptScore W2901681617C71829478 @default.
• W2901681617 hasConceptScore W2901681617C81885089 @default.
• W2901681617 hasConceptScore W2901681617C86339819 @default.
• W2901681617 hasConceptScore W2901681617C86803240 @default.
• W2901681617 hasConceptScore W2901681617C95444343 @default.
• W2901681617 hasFunder F4320320879 @default.
• W2901681617 hasIssue "1" @default.
• W2901681617 hasLocation W29016816171 @default.
• W2901681617 hasLocation W29016816172 @default.
• W2901681617 hasLocation W29016816173 @default.
• W2901681617 hasLocation W29016816174 @default.
• W2901681617 hasLocation W29016816175 @default.
• W2901681617 hasOpenAccess W2901681617 @default.
• W2901681617 hasPrimaryLocation W29016816171 @default.
• W2901681617 hasRelatedWork W2003069082 @default.
• W2901681617 hasRelatedWork W2057841153 @default.
• W2901681617 hasRelatedWork W2080937673 @default.
• W2901681617 hasRelatedWork W2107903378 @default.
• W2901681617 hasRelatedWork W2358952583 @default.
• W2901681617 hasRelatedWork W2810741294 @default.
• W2901681617 hasRelatedWork W3005079285 @default.
• W2901681617 hasRelatedWork W3178209818 @default.
• W2901681617 hasRelatedWork W4207021516 @default.
• W2901681617 hasRelatedWork W92947146 @default.

• next