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- W2901749215 abstract "Analysis of glycans via a porous graphitized carbon liquid chromatography (PGC-LC) coupled with electrospray ionization (tandem) mass spectrometry (ESI-MS(/MS)) is a powerful analytical method in the field of glycomics. Isobaric glycan structures can be identified reliably with the help of PGC-LC separation and subsequent identification by ESI-MS(/MS) in negative ion mode. In an effort to adapt PGC-LC-ESI-MS(/MS) to the nano-scale operation, spray instability along the nano-PGC-LC gradient was repeatedly observed on an LTQ Orbitrap Elite mass spectrometer equipped with a standard nano-electrospray ionization source. A stable electrospray was achieved with the implementation of a post-column make-up flow (PCMF). Thereby, acetonitrile was used to supplement the eluate from the nano-PGC-LC column. The improved spray stability enhanced detection and resolution of glycans during the analysis. This was in particular the case for smaller O-glycans which elute early in the high aqueous content regime of the nano-PGC-LC elution gradient. This study introduces PCMF as an easy-to-use instrumental adaptation to significantly improve spray stability in negative ion mode nano-PGC-LC-ESI-MS(/MS)-based analysis of glycans." @default.
- W2901749215 created "2018-11-29" @default.
- W2901749215 creator A5047918015 @default.
- W2901749215 creator A5052027825 @default.
- W2901749215 creator A5064775072 @default.
- W2901749215 creator A5082695733 @default.
- W2901749215 date "2018-11-23" @default.
- W2901749215 modified "2023-10-10" @default.
- W2901749215 title "Improvement of electrospray stability in negative ion mode for nano-PGC-LC-MS glycoanalysis via post-column make-up flow" @default.
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- W2901749215 doi "https://doi.org/10.1007/s10719-018-9848-1" @default.
- W2901749215 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30467791" @default.
- W2901749215 hasPublicationYear "2018" @default.
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