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• W2901781343 endingPage "133" @default.
• W2901781343 startingPage "119" @default.
• W2901781343 abstract "Contact lens wear carries a risk of complications, including corneal infection. Solving these complications has been hindered by limitations of existing animal models. Here, we report development of a new murine model of contact lens wear.C57BL/6 mice were fitted with custom-made silicone-hydrogel contact lenses with or without prior inoculation with Pseudomonas aeruginosa (PAO1-GFP). Contralateral eyes served as controls. Corneas were monitored for pathology, and examined ex vivo using high-magnification, time-lapse imaging. Fluorescent reporter mice allowed visualization of host cell membranes and immune cells. Lens-colonizing bacteria were detected by viable counts and FISH. Direct-colony PCR was used for bacterial identification.Without deliberate inoculation, lens-wearing corneas remained free of visible pathology, and retained a clarity similar to non-lens wearing controls. CD11c-YFP reporter mice revealed altered numbers, and distribution, of CD11c-positive cells in lens-wearing corneas after 24 h. Worn lenses showed bacterial colonization, primarily by known conjunctival or skin commensals. Corneal epithelial cells showed vacuolization during lens wear, and after 5 days, cells with phagocyte morphology appeared in the stroma that actively migrated over resident keratocytes that showed altered morphology. Immunofluorescence confirmed stromal Ly6G-positive cells after 5 days of lens wear, but not in MyD88 or IL-1R gene-knockout mice. P. aeruginosa-contaminated lenses caused infectious pathology in most mice from 1 to 13 days.This murine model of contact lens wear appears to faithfully mimic events occurring during human lens wear, and could be valuable for experiments, not possible in humans, that help solve the pathogenesis of lens-related complications." @default.
• W2901781343 created "2018-11-29" @default.
• W2901781343 creator A5003985370 @default.
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• W2901781343 creator A5089783930 @default.
• W2901781343 date "2019-01-01" @default.
• W2901781343 modified "2023-09-28" @default.
• W2901781343 title "A novel murine model for contact lens wear reveals clandestine IL-1R dependent corneal parainflammation and susceptibility to microbial keratitis upon inoculation with Pseudomonas aeruginosa" @default.
• W2901781343 cites W1168297851 @default.
• W2901781343 cites W1531692816 @default.
• W2901781343 cites W1595293335 @default.
• W2901781343 cites W1780716112 @default.
• W2901781343 cites W1855051021 @default.
• W2901781343 cites W1914593377 @default.
• W2901781343 cites W1959706298 @default.
• W2901781343 cites W1968931227 @default.
• W2901781343 cites W1969728970 @default.
• W2901781343 cites W1976215944 @default.
• W2901781343 cites W1981379434 @default.
• W2901781343 cites W1982360641 @default.
• W2901781343 cites W1983967590 @default.
• W2901781343 cites W1985759774 @default.
• W2901781343 cites W1986564074 @default.
• W2901781343 cites W1987132578 @default.
• W2901781343 cites W1990135825 @default.
• W2901781343 cites W1992232557 @default.
• W2901781343 cites W1997245466 @default.
• W2901781343 cites W2000228343 @default.
• W2901781343 cites W2001756396 @default.
• W2901781343 cites W2011719826 @default.
• W2901781343 cites W2015211917 @default.
• W2901781343 cites W2016592241 @default.
• W2901781343 cites W2022109868 @default.
• W2901781343 cites W2025019484 @default.
• W2901781343 cites W2025425706 @default.
• W2901781343 cites W2026510322 @default.
• W2901781343 cites W2030117818 @default.
• W2901781343 cites W2031084334 @default.
• W2901781343 cites W2032155429 @default.
• W2901781343 cites W2032227357 @default.
• W2901781343 cites W2034060753 @default.
• W2901781343 cites W2045036458 @default.
• W2901781343 cites W2055793978 @default.
• W2901781343 cites W2059447238 @default.
• W2901781343 cites W2063248639 @default.
• W2901781343 cites W2067976920 @default.
• W2901781343 cites W2068734008 @default.
• W2901781343 cites W2071553192 @default.
• W2901781343 cites W2071917782 @default.
• W2901781343 cites W2074616243 @default.
• W2901781343 cites W2075185464 @default.
• W2901781343 cites W2077808571 @default.
• W2901781343 cites W2082727434 @default.
• W2901781343 cites W2083189954 @default.
• W2901781343 cites W2083242879 @default.
• W2901781343 cites W2084277214 @default.
• W2901781343 cites W2085125986 @default.
• W2901781343 cites W2086675331 @default.
• W2901781343 cites W2093113195 @default.
• W2901781343 cites W2098950831 @default.
• W2901781343 cites W2104043604 @default.
• W2901781343 cites W2106141541 @default.
• W2901781343 cites W2110994794 @default.
• W2901781343 cites W2115242666 @default.
• W2901781343 cites W2122812109 @default.
• W2901781343 cites W2128503331 @default.
• W2901781343 cites W2130680119 @default.
• W2901781343 cites W2145373691 @default.
• W2901781343 cites W2155643696 @default.
• W2901781343 cites W2161001319 @default.
• W2901781343 cites W2163099661 @default.
• W2901781343 cites W2166813768 @default.
• W2901781343 cites W2206573395 @default.
• W2901781343 cites W2209285888 @default.
• W2901781343 cites W2252298505 @default.
• W2901781343 cites W2282376478 @default.
• W2901781343 cites W2330897937 @default.
• W2901781343 cites W2470477092 @default.
• W2901781343 cites W2473881938 @default.
• W2901781343 cites W2474156261 @default.
• W2901781343 cites W2519902791 @default.
• W2901781343 cites W2521057774 @default.
• W2901781343 cites W2548426831 @default.
• W2901781343 cites W2558931807 @default.
• W2901781343 cites W2591001641 @default.
• W2901781343 cites W2612188981 @default.
• W2901781343 cites W2736162768 @default.

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