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- W2901829291 abstract "Cell cycle phase transitions are tightly orchestrated to ensure efficient cell cycle progression and genome stability. Interrogating these transitions is important for understanding both normal and pathological cell proliferation. By quantifying the dynamics of the popular FUCCI reporters relative to the transitions into and out of S phase, we found that their dynamics are substantially and variably offset from true S phase boundaries. To enhance detection of phase transitions, we generated a new reporter whose oscillations are directly coupled to DNA replication and combined it with the FUCCI APC/C reporter to create “PIP-FUCCI”. The PIP degron fusion protein precisely marks the G1/S and S/G2 transitions; shows a rapid decrease in signal in response to large doses of DNA damage only during G1; and distinguishes cell type-specific and DNA damage source-dependent arrest phenotypes. We provide guidance to investigators in selecting appropriate fluorescent cell cycle reporters and new analysis strategies for delineating cell cycle transitions." @default.
- W2901829291 created "2018-11-29" @default.
- W2901829291 creator A5048863754 @default.
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- W2901829291 date "2018-11-17" @default.
- W2901829291 modified "2023-10-17" @default.
- W2901829291 title "Accurate delineation of cell cycle phase transitions in living cells with PIP-FUCCI" @default.
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- W2901829291 doi "https://doi.org/10.1080/15384101.2018.1547001" @default.
- W2901829291 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6342071" @default.
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