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- W2901968382 abstract "Pentacyclic triterpenes (PCTs) represent a major class of bioactive metabolites in banaba (Lagerstroemia speciosa) leaves; however, biosynthetic enzymes and their involvement in the temporal accumulation of PCTs remain to be studied. We use an integrated approach involving transcriptomics, metabolomics and gene function analysis to identify oxidosqualene cyclases (OSCs) and cytochrome P450 monooxygenases (P450s) that catalyzed sequential cyclization and oxidative reactions towards PCT scaffold diversification. Four monofunctional OSCs (LsOSC1,3–5) converted the triterpene precursor 2,3-oxidosqualene to either lupeol, β-amyrin or cycloartenol, and a multifunctional LsOSC2 formed α-amyrin as a major product along with β-amyrin. Two CYP716 family P450s (CYP716A265, CYP716A266) catalyzed C-28 oxidation of α-amyrin, β-amyrin and lupeol to form ursolic acid, oleanolic acid and betulinic acid, respectively. However, CYP716C55 catalyzed C-2α hydroxylation of ursolic acid and oleanolic acid to produce corosolic acid and maslinic acid, respectively. Besides, combined transcript and metabolite analysis suggested major roles for the LsOSC2, CYP716A265 and CYP716C55 in determining leaf ursane and oleanane profiles. Combinatorial expression of OSCs and CYP716s in Saccharomyces cerevisiae and Nicotiana benthamiana led to PCT pathway reconstruction, signifying the utility of banaba enzymes for bioactive PCT production in alternate plant/microbial hosts that are more easily tractable than the tree species." @default.
- W2901968382 created "2018-11-29" @default.
- W2901968382 creator A5007994908 @default.
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- W2901968382 creator A5057458809 @default.
- W2901968382 creator A5068326827 @default.
- W2901968382 date "2018-12-20" @default.
- W2901968382 modified "2023-10-14" @default.
- W2901968382 title "Oxidosqualene cyclase and CYP716 enzymes contribute to triterpene structural diversity in the medicinal tree banaba" @default.
- W2901968382 cites W1211954221 @default.
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- W2901968382 cites W1546738772 @default.
- W2901968382 cites W1577577364 @default.
- W2901968382 cites W1777456466 @default.
- W2901968382 cites W1972892672 @default.
- W2901968382 cites W1980051133 @default.
- W2901968382 cites W1983433251 @default.
- W2901968382 cites W1983779245 @default.
- W2901968382 cites W1984565512 @default.
- W2901968382 cites W1987499555 @default.
- W2901968382 cites W2003766374 @default.
- W2901968382 cites W2003892466 @default.
- W2901968382 cites W2036028864 @default.
- W2901968382 cites W2038591876 @default.
- W2901968382 cites W2041184252 @default.
- W2901968382 cites W2041987863 @default.
- W2901968382 cites W2044717234 @default.
- W2901968382 cites W2048789705 @default.
- W2901968382 cites W2049773266 @default.
- W2901968382 cites W2053689785 @default.
- W2901968382 cites W2054794298 @default.
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- W2901968382 cites W2077436426 @default.
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- W2901968382 cites W2091602891 @default.
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- W2901968382 cites W2105423509 @default.
- W2901968382 cites W2107705582 @default.
- W2901968382 cites W2108479449 @default.
- W2901968382 cites W2109743008 @default.
- W2901968382 cites W2110043521 @default.
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- W2901968382 cites W2134266820 @default.
- W2901968382 cites W2141542965 @default.
- W2901968382 cites W2144420193 @default.
- W2901968382 cites W2148058118 @default.
- W2901968382 cites W2152207030 @default.
- W2901968382 cites W2154398258 @default.
- W2901968382 cites W2155657075 @default.
- W2901968382 cites W2156125289 @default.
- W2901968382 cites W2158359278 @default.
- W2901968382 cites W2160164080 @default.
- W2901968382 cites W2160919709 @default.
- W2901968382 cites W2221023436 @default.
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- W2901968382 cites W2286908285 @default.
- W2901968382 cites W2306635261 @default.
- W2901968382 cites W2318910178 @default.
- W2901968382 cites W2346486524 @default.
- W2901968382 cites W2408221009 @default.
- W2901968382 cites W2409362704 @default.
- W2901968382 cites W2417778088 @default.
- W2901968382 cites W2507789109 @default.
- W2901968382 cites W2509940547 @default.
- W2901968382 cites W2517916592 @default.
- W2901968382 cites W2525852379 @default.
- W2901968382 cites W2569729391 @default.
- W2901968382 cites W2586211853 @default.
- W2901968382 cites W2588403571 @default.
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- W2901968382 cites W2768397451 @default.
- W2901968382 cites W2770446418 @default.
- W2901968382 cites W2774029784 @default.
- W2901968382 cites W2786331742 @default.
- W2901968382 cites W2793245773 @default.
- W2901968382 cites W4206799458 @default.
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- W2901968382 doi "https://doi.org/10.1111/nph.15606" @default.
- W2901968382 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30472753" @default.
- W2901968382 hasPublicationYear "2018" @default.
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