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• W2902133313 endingPage "357" @default.
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• W2902133313 abstract "Cell deformability is a label-free biomarker of cell state in physiological and disease contexts ranging from stem cell differentiation to cancer progression. Harnessing deformability as a phenotype for screening applications requires a method that can simultaneously measure the deformability of hundreds of cell samples and can interface with existing high throughput facilities. Here we present a scalable cell filtration device, which relies on the pressure-driven deformation of cells through a series of pillars that are separated by micron-scale gaps on the timescale of seconds: less deformable cells occlude the gaps more readily than more deformable cells, resulting in decreased filtrate volume which is measured using a plate reader. The key innovation in this method is that we design customized arrays of individual filtration devices in a standard 96-well format using soft lithography, which enables multiwell input samples and filtrate outputs to be processed with higher throughput using automated pipette arrays and plate readers. To validate high throughput filtration to detect changes in cell deformability, we show the differential filtration of human ovarian cancer cells that have acquired cisplatin-resistance, which is corroborated with cell stiffness measurements using quantitative deformability cytometry. We also demonstrate differences in the filtration of human cancer cell lines, including ovarian cancer cells that overexpress transcription factors (Snail, Slug), which are implicated in epithelial-to-mesenchymal transition; breast cancer cells (malignant versus benign); and prostate cancer cells (highly versus weekly metastatic). We additionally show how the filtration of ovarian cancer cells is affected by treatment with drugs known to perturb the cytoskeleton and the nucleus. Our results across multiple cancer cell types with both genetic and pharmacologic manipulations demonstrate the potential of this scalable filtration device to screen cells based on their deformability." @default.
• W2902133313 created "2018-12-11" @default.
• W2902133313 creator A5005772693 @default.
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• W2902133313 creator A5072088187 @default.
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• W2902133313 creator A5074884103 @default.
• W2902133313 creator A5082993956 @default.
• W2902133313 date "2019-01-01" @default.
• W2902133313 modified "2023-10-14" @default.
• W2902133313 title "A scalable filtration method for high throughput screening based on cell deformability" @default.
• W2902133313 cites W1549239847 @default.
• W2902133313 cites W1555009525 @default.
• W2902133313 cites W1657255525 @default.
• W2902133313 cites W1760501277 @default.
• W2902133313 cites W1840209374 @default.
• W2902133313 cites W1914429793 @default.
• W2902133313 cites W1944193636 @default.
• W2902133313 cites W1963785221 @default.
• W2902133313 cites W1971375063 @default.
• W2902133313 cites W1972554719 @default.
• W2902133313 cites W1973089587 @default.
• W2902133313 cites W1976814602 @default.
• W2902133313 cites W1978435155 @default.
• W2902133313 cites W1978500894 @default.
• W2902133313 cites W1978705679 @default.
• W2902133313 cites W1979777072 @default.
• W2902133313 cites W1980876955 @default.
• W2902133313 cites W1982964683 @default.
• W2902133313 cites W1991001144 @default.
• W2902133313 cites W1992648778 @default.
• W2902133313 cites W1993910159 @default.
• W2902133313 cites W1995834426 @default.
• W2902133313 cites W1996152000 @default.
• W2902133313 cites W1998173416 @default.
• W2902133313 cites W2002373478 @default.
• W2902133313 cites W2004349971 @default.
• W2902133313 cites W2005490888 @default.
• W2902133313 cites W2015383096 @default.
• W2902133313 cites W2016983464 @default.
• W2902133313 cites W2018218670 @default.
• W2902133313 cites W2018784030 @default.
• W2902133313 cites W2019237997 @default.
• W2902133313 cites W2020216997 @default.
• W2902133313 cites W2024952704 @default.
• W2902133313 cites W2026908430 @default.
• W2902133313 cites W2027428545 @default.
• W2902133313 cites W2029446957 @default.
• W2902133313 cites W2032853423 @default.
• W2902133313 cites W2036631612 @default.
• W2902133313 cites W2039707521 @default.
• W2902133313 cites W2047327876 @default.
• W2902133313 cites W2054825107 @default.
• W2902133313 cites W2055099137 @default.
• W2902133313 cites W2056210736 @default.
• W2902133313 cites W2058293803 @default.
• W2902133313 cites W2062650678 @default.
• W2902133313 cites W2063532871 @default.
• W2902133313 cites W2065524217 @default.
• W2902133313 cites W2066030148 @default.
• W2902133313 cites W2075489095 @default.
• W2902133313 cites W2084781720 @default.
• W2902133313 cites W2090588873 @default.
• W2902133313 cites W2092412094 @default.
• W2902133313 cites W2093977679 @default.
• W2902133313 cites W2095192232 @default.
• W2902133313 cites W2096512070 @default.
• W2902133313 cites W2097038959 @default.
• W2902133313 cites W2097374235 @default.
• W2902133313 cites W2100113004 @default.
• W2902133313 cites W2101425786 @default.
• W2902133313 cites W2101907683 @default.
• W2902133313 cites W2102099949 @default.
• W2902133313 cites W2102382046 @default.
• W2902133313 cites W2104941124 @default.
• W2902133313 cites W2110344693 @default.
• W2902133313 cites W2110781930 @default.
• W2902133313 cites W2116413963 @default.
• W2902133313 cites W2116577936 @default.
• W2902133313 cites W2124633704 @default.
• W2902133313 cites W2133447826 @default.
• W2902133313 cites W2140974583 @default.
• W2902133313 cites W2140991469 @default.
• W2902133313 cites W2141114538 @default.
• W2902133313 cites W2141235922 @default.
• W2902133313 cites W2144291883 @default.
• W2902133313 cites W2144373650 @default.
• W2902133313 cites W2146337514 @default.
• W2902133313 cites W2148528535 @default.
• W2902133313 cites W2149184691 @default.

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