FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2902307150> ?p ?o ?g. }

• W2902307150 endingPage "217" @default.
• W2902307150 startingPage "213" @default.
• W2902307150 abstract "We report a novel method named LITPOMS (ligand titration, fast photochemical oxidation of proteins and mass spectrometry) to characterize protein-ligand binding stoichiometry, binding sites, and site-specific binding constants. The system used to test the method is melittin–calmodulin, in which the peptide melittin binds to calcium-bound calmodulin. Global-level measurements reveal the binding stoichiometry of 1:1 whereas peptide-level data coupled with fitting reveal the binding sites and the site-specific binding affinity. Moreover, we extended the analysis to the residue level and identified six critical binding residues. The results show that melittin binds to the N-terminal, central linker, and C-terminal regions of holo-calmodulin with an affinity of 4.6 nM, in agreement with results of previous studies. LITPOMS, for the first time, brings high residue-level resolution to affinity measurements, providing simultaneously qualitative and quantitative understanding of protein-ligand binding. The approach can be expanded to other binding systems without tagging the protein to give high spatial resolution." @default.
• W2902307150 created "2018-12-11" @default.
• W2902307150 creator A5031479644 @default.
• W2902307150 creator A5054440913 @default.
• W2902307150 creator A5056480326 @default.
• W2902307150 creator A5076508053 @default.
• W2902307150 date "2018-11-27" @default.
• W2902307150 modified "2023-10-06" @default.
• W2902307150 title "Protein-Ligand Interaction by Ligand Titration, Fast Photochemical Oxidation of Proteins and Mass Spectrometry: LITPOMS" @default.
• W2902307150 cites W1983065854 @default.
• W2902307150 cites W1985020303 @default.
• W2902307150 cites W1986493103 @default.
• W2902307150 cites W1997997916 @default.
• W2902307150 cites W2009247981 @default.
• W2902307150 cites W2020357919 @default.
• W2902307150 cites W2023096047 @default.
• W2902307150 cites W2023382565 @default.
• W2902307150 cites W2024849484 @default.
• W2902307150 cites W2028931227 @default.
• W2902307150 cites W2037462278 @default.
• W2902307150 cites W2040819214 @default.
• W2902307150 cites W2044228723 @default.
• W2902307150 cites W2049265493 @default.
• W2902307150 cites W2055575114 @default.
• W2902307150 cites W2070989322 @default.
• W2902307150 cites W2080446617 @default.
• W2902307150 cites W2081470364 @default.
• W2902307150 cites W2083449193 @default.
• W2902307150 cites W2090069710 @default.
• W2902307150 cites W2121830147 @default.
• W2902307150 cites W2124112137 @default.
• W2902307150 cites W2146686514 @default.
• W2902307150 cites W2155258555 @default.
• W2902307150 cites W2159307624 @default.
• W2902307150 cites W2211424641 @default.
• W2902307150 cites W2253286196 @default.
• W2902307150 cites W2476071496 @default.
• W2902307150 cites W2493819921 @default.
• W2902307150 cites W2754079461 @default.
• W2902307150 cites W2754229710 @default.
• W2902307150 cites W2786824042 @default.
• W2902307150 cites W2801782896 @default.
• W2902307150 cites W2803314045 @default.
• W2902307150 cites W4248721956 @default.
• W2902307150 doi "https://doi.org/10.1007/s13361-018-2076-x" @default.
• W2902307150 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6438201" @default.
• W2902307150 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30484077" @default.
• W2902307150 hasPublicationYear "2018" @default.
• W2902307150 type Work @default.
• W2902307150 sameAs 2902307150 @default.
• W2902307150 citedByCount "16" @default.
• W2902307150 countsByYear W29023071502019 @default.
• W2902307150 countsByYear W29023071502020 @default.
• W2902307150 countsByYear W29023071502021 @default.
• W2902307150 countsByYear W29023071502022 @default.
• W2902307150 countsByYear W29023071502023 @default.
• W2902307150 crossrefType "journal-article" @default.
• W2902307150 hasAuthorship W2902307150A5031479644 @default.
• W2902307150 hasAuthorship W2902307150A5054440913 @default.
• W2902307150 hasAuthorship W2902307150A5056480326 @default.
• W2902307150 hasAuthorship W2902307150A5076508053 @default.
• W2902307150 hasBestOaLocation W29023071502 @default.
• W2902307150 hasConcept C107824862 @default.
• W2902307150 hasConcept C109095088 @default.
• W2902307150 hasConcept C111919701 @default.
• W2902307150 hasConcept C116569031 @default.
• W2902307150 hasConcept C162356407 @default.
• W2902307150 hasConcept C170493617 @default.
• W2902307150 hasConcept C178790620 @default.
• W2902307150 hasConcept C179104552 @default.
• W2902307150 hasConcept C184866935 @default.
• W2902307150 hasConcept C185592680 @default.
• W2902307150 hasConcept C2778001312 @default.
• W2902307150 hasConcept C2779281246 @default.
• W2902307150 hasConcept C2780557392 @default.
• W2902307150 hasConcept C29688787 @default.
• W2902307150 hasConcept C41008148 @default.
• W2902307150 hasConcept C43617362 @default.
• W2902307150 hasConcept C519063684 @default.
• W2902307150 hasConcept C55493867 @default.
• W2902307150 hasConceptScore W2902307150C107824862 @default.
• W2902307150 hasConceptScore W2902307150C109095088 @default.
• W2902307150 hasConceptScore W2902307150C111919701 @default.
• W2902307150 hasConceptScore W2902307150C116569031 @default.
• W2902307150 hasConceptScore W2902307150C162356407 @default.
• W2902307150 hasConceptScore W2902307150C170493617 @default.
• W2902307150 hasConceptScore W2902307150C178790620 @default.
• W2902307150 hasConceptScore W2902307150C179104552 @default.
• W2902307150 hasConceptScore W2902307150C184866935 @default.
• W2902307150 hasConceptScore W2902307150C185592680 @default.
• W2902307150 hasConceptScore W2902307150C2778001312 @default.
• W2902307150 hasConceptScore W2902307150C2779281246 @default.
• W2902307150 hasConceptScore W2902307150C2780557392 @default.
• W2902307150 hasConceptScore W2902307150C29688787 @default.
• W2902307150 hasConceptScore W2902307150C41008148 @default.
• W2902307150 hasConceptScore W2902307150C43617362 @default.
• W2902307150 hasConceptScore W2902307150C519063684 @default.
• W2902307150 hasConceptScore W2902307150C55493867 @default.

• next