FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2902547680> ?p ?o ?g. }

• W2902547680 endingPage "1217" @default.
• W2902547680 startingPage "1202" @default.
• W2902547680 abstract "Outer membrane vesicles produced by Gram-negative bacteria have been studied for half a century but the possibility that Gram-positive bacteria secrete extracellular vesicles (EVs) was not pursued until recently due to the assumption that the thick peptidoglycan cell wall would prevent their release to the environment. However, following their discovery in fungi, which also have cell walls, EVs have now been described for a variety of Gram-positive bacteria. EVs purified from Gram-positive bacteria are implicated in virulence, toxin release, and transference to host cells, eliciting immune responses, and spread of antibiotic resistance. Listeria monocytogenes is a Gram-positive bacterium that causes listeriosis. Here we report that L. monocytogenes produces EVs with diameters ranging from 20 to 200 nm, containing the pore-forming toxin listeriolysin O (LLO) and phosphatidylinositol-specific phospholipase C (PI-PLC). Cell-free EV preparations were toxic to mammalian cells, the murine macrophage cell line J774.16, in a LLO-dependent manner, evidencing EV biological activity. The deletion of plcA increased EV toxicity, suggesting PI-PLC reduced LLO activity. Using simultaneous metabolite, protein, and lipid extraction (MPLEx) multiomics we characterized protein, lipid, and metabolite composition of bacterial cells and secreted EVs and found that EVs carry the majority of listerial virulence proteins. Using immunogold EM we detected LLO at several organelles within infected human epithelial cells and with high-resolution fluorescence imaging we show that dynamic lipid structures are released from L. monocytogenes during infection. Our findings demonstrate that L. monocytogenes uses EVs for toxin release and implicate these structures in mammalian cytotoxicity." @default.
• W2902547680 created "2018-12-11" @default.
• W2902547680 creator A5000784879 @default.
• W2902547680 creator A5009421476 @default.
• W2902547680 creator A5012660702 @default.
• W2902547680 creator A5015324999 @default.
• W2902547680 creator A5025745502 @default.
• W2902547680 creator A5028627477 @default.
• W2902547680 creator A5031285183 @default.
• W2902547680 creator A5037253743 @default.
• W2902547680 creator A5059796157 @default.
• W2902547680 creator A5063093139 @default.
• W2902547680 creator A5072292859 @default.
• W2902547680 creator A5077902719 @default.
• W2902547680 creator A5079820905 @default.
• W2902547680 creator A5080497162 @default.
• W2902547680 creator A5081128029 @default.
• W2902547680 creator A5090535168 @default.
• W2902547680 date "2019-01-01" @default.
• W2902547680 modified "2023-10-15" @default.
• W2902547680 title "Listeria monocytogenes virulence factors, including listeriolysin O, are secreted in biologically active extracellular vesicles" @default.
• W2902547680 cites W1270356811 @default.
• W2902547680 cites W1593297828 @default.
• W2902547680 cites W1634136431 @default.
• W2902547680 cites W1892471227 @default.
• W2902547680 cites W1909750055 @default.
• W2902547680 cites W1929878434 @default.
• W2902547680 cites W1948610453 @default.
• W2902547680 cites W1958221753 @default.
• W2902547680 cites W1969139980 @default.
• W2902547680 cites W1971656321 @default.
• W2902547680 cites W1973509179 @default.
• W2902547680 cites W1976327004 @default.
• W2902547680 cites W1976456732 @default.
• W2902547680 cites W1989299822 @default.
• W2902547680 cites W2002770775 @default.
• W2902547680 cites W2011736725 @default.
• W2902547680 cites W2016918522 @default.
• W2902547680 cites W2019402080 @default.
• W2902547680 cites W2019615869 @default.
• W2902547680 cites W2020009446 @default.
• W2902547680 cites W2023459461 @default.
• W2902547680 cites W2027426716 @default.
• W2902547680 cites W2032911095 @default.
• W2902547680 cites W2033126129 @default.
• W2902547680 cites W2034539700 @default.
• W2902547680 cites W2034583414 @default.
• W2902547680 cites W2047973355 @default.
• W2902547680 cites W2048190893 @default.
• W2902547680 cites W2059210701 @default.
• W2902547680 cites W2062057730 @default.
• W2902547680 cites W2063490043 @default.
• W2902547680 cites W2067579927 @default.
• W2902547680 cites W2067820603 @default.
• W2902547680 cites W2069559774 @default.
• W2902547680 cites W2071673208 @default.
• W2902547680 cites W2073273682 @default.
• W2902547680 cites W2076198022 @default.
• W2902547680 cites W2076277268 @default.
• W2902547680 cites W2093417734 @default.
• W2902547680 cites W2094070743 @default.
• W2902547680 cites W2094692479 @default.
• W2902547680 cites W2098964008 @default.
• W2902547680 cites W2099089752 @default.
• W2902547680 cites W2107957830 @default.
• W2902547680 cites W2116537957 @default.
• W2902547680 cites W2117411464 @default.
• W2902547680 cites W2120556801 @default.
• W2902547680 cites W2120640969 @default.
• W2902547680 cites W2122857784 @default.
• W2902547680 cites W2125636530 @default.
• W2902547680 cites W2128726565 @default.
• W2902547680 cites W2129515516 @default.
• W2902547680 cites W2131967845 @default.
• W2902547680 cites W2132613162 @default.
• W2902547680 cites W2135130533 @default.
• W2902547680 cites W2144014651 @default.
• W2902547680 cites W2147830145 @default.
• W2902547680 cites W2154543397 @default.
• W2902547680 cites W2155183797 @default.
• W2902547680 cites W2157249590 @default.
• W2902547680 cites W2178043251 @default.
• W2902547680 cites W2236777032 @default.
• W2902547680 cites W2272655888 @default.
• W2902547680 cites W2294941861 @default.
• W2902547680 cites W2391375401 @default.
• W2902547680 cites W2541748448 @default.
• W2902547680 cites W2544360569 @default.
• W2902547680 cites W2583344557 @default.
• W2902547680 cites W2610518490 @default.
• W2902547680 cites W2736457596 @default.
• W2902547680 cites W2792276960 @default.
• W2902547680 cites W2899663373 @default.
• W2902547680 doi "https://doi.org/10.1074/jbc.ra118.006472" @default.
• W2902547680 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6349127" @default.
• W2902547680 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30504226" @default.
• W2902547680 hasPublicationYear "2019" @default.
• W2902547680 type Work @default.

• next