FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2902890517> ?p ?o ?g. }

• W2902890517 endingPage "88" @default.
• W2902890517 startingPage "83" @default.
• W2902890517 abstract "An increase in allergen-specific IgG4 (sIgG4), which serves as a blocking antibody, is associated with acquisition of immune tolerance after immunotherapy. In this study, we developed a rapid, sensitive, and homogeneous immunoassay based on the light-initiated chemiluminescent assay (LICA) technology for quantifying allergen sIgG4 in serum samples.Allergen sIgG4 was measured in vitro by incubating the sample with biotinylated allergens and chemiluminescent beads coated with anti-human IgG4 antibody, followed by the addition of streptavidin-coated sensitizer beads. Multiple tests were performed to optimize the working conditions of the LICA and evaluate its performance.We established the optimal concentration of biotinylated allergens (250 ng/mL), the optimal dilution range (1:8 for Gal d 1, Gal d 2 sIgG4 and 1:4 for Gal d 3, Gal d 4 sIgG4), and the optimal incubation time (20 min for Gal d 1, Gal d 2 sIgG4 and 40 min for Gal d 3, Gal d 4 sIgG4). The lower limit of quantification (LLOQ) was 0.261 ng/mL. The coefficient variation (CV) of the LICA was <10%. The assay was unaffected by general interfering substances at physiological concentrations. It exhibited excellent accuracy to detect allergen-sIgG4 in human serum. Additionally, we demonstrated that the levels of Gal d 1, Gal d 2, and Gal d 3-sIgG4 were significantly higher in the egg allergy group (p < .05), but no differences were found between the groups for Gal d 4-sIgG4.The LICA demonstrated satisfactory performance and can be used for quantifying allergen sIgG4 in clinical practice." @default.
• W2902890517 created "2018-12-11" @default.
• W2902890517 creator A5002876717 @default.
• W2902890517 creator A5015434730 @default.
• W2902890517 creator A5027650839 @default.
• W2902890517 creator A5035149310 @default.
• W2902890517 creator A5072394119 @default.
• W2902890517 creator A5083333083 @default.
• W2902890517 creator A5089458129 @default.
• W2902890517 date "2019-02-01" @default.
• W2902890517 modified "2023-09-24" @default.
• W2902890517 title "A light-initiated chemiluminescent assay for rapid quantitation of allergen-specific IgG4 in clinical samples" @default.
• W2902890517 cites W1131182843 @default.
• W2902890517 cites W1482580092 @default.
• W2902890517 cites W1936218468 @default.
• W2902890517 cites W1968796016 @default.
• W2902890517 cites W1976978141 @default.
• W2902890517 cites W1984188413 @default.
• W2902890517 cites W1991782145 @default.
• W2902890517 cites W1999409387 @default.
• W2902890517 cites W2007281461 @default.
• W2902890517 cites W2012513978 @default.
• W2902890517 cites W2069780509 @default.
• W2902890517 cites W2093112435 @default.
• W2902890517 cites W2098557641 @default.
• W2902890517 cites W2099281419 @default.
• W2902890517 cites W2105176378 @default.
• W2902890517 cites W2123061071 @default.
• W2902890517 cites W2128601434 @default.
• W2902890517 cites W2139821422 @default.
• W2902890517 cites W2143718984 @default.
• W2902890517 cites W2156678619 @default.
• W2902890517 cites W2158673466 @default.
• W2902890517 cites W2208443141 @default.
• W2902890517 cites W2312138331 @default.
• W2902890517 cites W2323871271 @default.
• W2902890517 cites W2345208131 @default.
• W2902890517 cites W2511945847 @default.
• W2902890517 cites W2518437768 @default.
• W2902890517 cites W2530562429 @default.
• W2902890517 cites W2589248587 @default.
• W2902890517 cites W2738905037 @default.
• W2902890517 cites W2755373010 @default.
• W2902890517 cites W2770868505 @default.
• W2902890517 cites W2771883442 @default.
• W2902890517 cites W2792993553 @default.
• W2902890517 cites W4238668236 @default.
• W2902890517 doi "https://doi.org/10.1016/j.cca.2018.11.036" @default.
• W2902890517 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30517849" @default.
• W2902890517 hasPublicationYear "2019" @default.
• W2902890517 type Work @default.
• W2902890517 sameAs 2902890517 @default.
• W2902890517 citedByCount "9" @default.
• W2902890517 countsByYear W29028905172020 @default.
• W2902890517 countsByYear W29028905172022 @default.
• W2902890517 countsByYear W29028905172023 @default.
• W2902890517 crossrefType "journal-article" @default.
• W2902890517 hasAuthorship W2902890517A5002876717 @default.
• W2902890517 hasAuthorship W2902890517A5015434730 @default.
• W2902890517 hasAuthorship W2902890517A5027650839 @default.
• W2902890517 hasAuthorship W2902890517A5035149310 @default.
• W2902890517 hasAuthorship W2902890517A5072394119 @default.
• W2902890517 hasAuthorship W2902890517A5083333083 @default.
• W2902890517 hasAuthorship W2902890517A5089458129 @default.
• W2902890517 hasConcept C145802702 @default.
• W2902890517 hasConcept C159654299 @default.
• W2902890517 hasConcept C185592680 @default.
• W2902890517 hasConcept C203014093 @default.
• W2902890517 hasConcept C204628709 @default.
• W2902890517 hasConcept C207480886 @default.
• W2902890517 hasConcept C2776190903 @default.
• W2902890517 hasConcept C2778204606 @default.
• W2902890517 hasConcept C2780420688 @default.
• W2902890517 hasConcept C2780510475 @default.
• W2902890517 hasConcept C43617362 @default.
• W2902890517 hasConcept C55493867 @default.
• W2902890517 hasConcept C71924100 @default.
• W2902890517 hasConceptScore W2902890517C145802702 @default.
• W2902890517 hasConceptScore W2902890517C159654299 @default.
• W2902890517 hasConceptScore W2902890517C185592680 @default.
• W2902890517 hasConceptScore W2902890517C203014093 @default.
• W2902890517 hasConceptScore W2902890517C204628709 @default.
• W2902890517 hasConceptScore W2902890517C207480886 @default.
• W2902890517 hasConceptScore W2902890517C2776190903 @default.
• W2902890517 hasConceptScore W2902890517C2778204606 @default.
• W2902890517 hasConceptScore W2902890517C2780420688 @default.
• W2902890517 hasConceptScore W2902890517C2780510475 @default.
• W2902890517 hasConceptScore W2902890517C43617362 @default.
• W2902890517 hasConceptScore W2902890517C55493867 @default.
• W2902890517 hasConceptScore W2902890517C71924100 @default.
• W2902890517 hasFunder F4320321001 @default.
• W2902890517 hasLocation W29028905171 @default.
• W2902890517 hasLocation W29028905172 @default.
• W2902890517 hasOpenAccess W2902890517 @default.
• W2902890517 hasPrimaryLocation W29028905171 @default.
• W2902890517 hasRelatedWork W141881406 @default.
• W2902890517 hasRelatedWork W2015699218 @default.
• W2902890517 hasRelatedWork W2033769456 @default.

• next