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• W2902980384 abstract "Microvascular perfusion dynamics are vital to physiological function and are frequently dysregulated in injury and disease. Typically studies measure microvascular flow in a few selected vascular segments over limited time, failing to capture spatial and temporal variability. To quantify microvascular flow in a more complete and unbiased way we developed STAFF (Spatial Temporal Analysis of Fieldwise Flow), a macro for FIJI open-source image analysis software. Using high-speed microvascular flow movies, STAFF generates kymographs for every time interval for every vascular segment, calculates flow velocities from red blood cell shadow angles, and outputs the data as color-coded velocity map movies and spreadsheets. In untreated mice, analyses demonstrated profound variation even between adjacent sinusoids over seconds. In acetaminophen-treated mice we detected flow reduction localized to pericentral regions. STAFF is a powerful new tool capable of providing novel insights by enabling measurement of the complex spatiotemporal dynamics of microvascular flow." @default.
• W2902980384 created "2018-12-11" @default.
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• W2902980384 date "2019-05-01" @default.
• W2902980384 modified "2023-09-27" @default.
• W2902980384 title "A simple automated method for continuous fieldwise measurement of microvascular hemodynamics" @default.
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• W2902980384 doi "https://doi.org/10.1016/j.mvr.2018.11.010" @default.
• W2902980384 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6379124" @default.
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