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- W2903014412 abstract "3459 Virulent bacterial pathogens use differential mechanisms to enter the cells to establish chronic infections and inflammation associated with epithelial cell damage that may lead to development of cancer in susceptible individuals. Dr adhesins of Escherichia coli (E.coli) are associated with recurrent urinary tract infections as well as with diarrheal disease. Dr adhesins bind to decay accelerating factor (DAF), a complement regulatory and signaling molecule on host cells and their binding is under the control of hormones. Previously, we reported that pretreatment with physiological concentration of 17-β estradiol protects Huh-7 hepatoma cells and primary human hepatocytes against Dr E.coli invasion (about 60% reduced invasion). This protection was abrogated by blocking with estrogen receptor (ER) antagonist ICI182, 780 (1uM) suggesting involvement of ERs. Evidence suggests that estrogen may also cause rapid activation of signal transduction pathways like mitogen-activated protein kinase (MAPK) pathways involving ERs. Therefore in the present study, we investigated the role of MAPK dependent pathway in estrogen mediated protection in Huh-7cells against Dr E.coli invasion. Blocking studies were performed by pretreating cells with MEK1 inhibitor (PD98059) or p38 MAPK inhibitor (SB 202190) prior to bacterial invasion assay using gentamicin to kill extracellular bacteria. Percent bacterial colony forming units (CFU) internalized after treatment with three different doses of inhibitors was analyzed. A combined effect of PD98059 or SB 202190 with ER antagonist ICI 182,780 was also investigated. Pretreatment with PD98059 or SB 202190 showed partial reversal of estrogen mediated protection by increasing internalized mean percent bacterial CFU than in cells treated with estrogen alone (54 to 59.66 ±4 (mean±SEM) vs control 33 to 40±4). Values are expressed as mean percent bacterial CFU of untreated control considered as hundred percent. With PD98059, the results were significant at 0.01uM (p=0.0009) and at 0.1uM (p" @default.
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- W2903014412 date "2007-05-01" @default.
- W2903014412 modified "2023-09-27" @default.
- W2903014412 title "Estrogen mediates protection against bacterial invasion on Huh-7 cells via both estrogen receptor and mitogen-activated protein kinase dependent pathway" @default.
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