FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2903065790> ?p ?o ?g. }

• W2903065790 endingPage "462" @default.
• W2903065790 startingPage "453" @default.
• W2903065790 abstract "The N-linked glycan in immunoglobulin G is critical for the stability and function of the crystallizable fragment (Fc) region. Alteration of these protein properties upon the removal of the N-linked glycan has often been explained by the alteration of the CH2 domain orientation in the Fc region. To confirm this hypothesis, we examined the small-angle X-ray scattering (SAXS) profile of the glycosylated Fc region (gFc) and aglycosylated Fc region (aFc) in solution. Conformational characteristics of the CH2 domain orientation were validated by comparison with SAXS profiles theoretically calculated from multiple crystal structures of the Fc region with different CH2 domain orientations. The reduced chi-square values from the fitting analyses of gFc and aFc associated with the degree of openness or closure of each crystal structure, as determined from the first principal component that partially governed the variation of the CH2 domain orientation extracted by a singular value decomposition analysis. For both gFc and aFc, the best-fitted SAXS profiles corresponded to ones calculated based on the crystal structure of gFc that formed a “semi-closed” CH2 domain orientation. Collectively, the data indicated that the removal of the N-linked glycan only negligibly affected the CH2 domain orientation in solution. These findings will guide the development of methodology for the production of highly refined functional Fc variants." @default.
• W2903065790 created "2018-12-11" @default.
• W2903065790 creator A5015856863 @default.
• W2903065790 creator A5027321457 @default.
• W2903065790 creator A5035357296 @default.
• W2903065790 creator A5070776413 @default.
• W2903065790 date "2018-12-12" @default.
• W2903065790 modified "2023-10-16" @default.
• W2903065790 title "C_H2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering" @default.
• W2903065790 cites W1607931568 @default.
• W2903065790 cites W1825768998 @default.
• W2903065790 cites W1831036856 @default.
• W2903065790 cites W1885817024 @default.
• W2903065790 cites W1898469614 @default.
• W2903065790 cites W1929486047 @default.
• W2903065790 cites W1967734507 @default.
• W2903065790 cites W1969638854 @default.
• W2903065790 cites W1969824500 @default.
• W2903065790 cites W1971166275 @default.
• W2903065790 cites W1973718144 @default.
• W2903065790 cites W1974302168 @default.
• W2903065790 cites W1975082171 @default.
• W2903065790 cites W1975834815 @default.
• W2903065790 cites W1979816062 @default.
• W2903065790 cites W1986404254 @default.
• W2903065790 cites W1989407795 @default.
• W2903065790 cites W1999602967 @default.
• W2903065790 cites W1999989255 @default.
• W2903065790 cites W2000693605 @default.
• W2903065790 cites W2004286543 @default.
• W2903065790 cites W2006903057 @default.
• W2903065790 cites W2011138965 @default.
• W2903065790 cites W2018038154 @default.
• W2903065790 cites W2018816634 @default.
• W2903065790 cites W2020125060 @default.
• W2903065790 cites W2022754521 @default.
• W2903065790 cites W2025966090 @default.
• W2903065790 cites W2028588417 @default.
• W2903065790 cites W2032419556 @default.
• W2903065790 cites W2032552806 @default.
• W2903065790 cites W2034515273 @default.
• W2903065790 cites W2035179120 @default.
• W2903065790 cites W2037007095 @default.
• W2903065790 cites W2038876401 @default.
• W2903065790 cites W2039928024 @default.
• W2903065790 cites W2041479891 @default.
• W2903065790 cites W2045193638 @default.
• W2903065790 cites W2050405684 @default.
• W2903065790 cites W2058232936 @default.
• W2903065790 cites W2060204419 @default.
• W2903065790 cites W2066088601 @default.
• W2903065790 cites W2071129921 @default.
• W2903065790 cites W2071702057 @default.
• W2903065790 cites W2072081310 @default.
• W2903065790 cites W2072086606 @default.
• W2903065790 cites W2072927989 @default.
• W2903065790 cites W2073900740 @default.
• W2903065790 cites W2077069177 @default.
• W2903065790 cites W2078624174 @default.
• W2903065790 cites W2085206766 @default.
• W2903065790 cites W2086437061 @default.
• W2903065790 cites W2094445987 @default.
• W2903065790 cites W2096387523 @default.
• W2903065790 cites W2106915248 @default.
• W2903065790 cites W2107505802 @default.
• W2903065790 cites W2112157366 @default.
• W2903065790 cites W2116339520 @default.
• W2903065790 cites W2121107218 @default.
• W2903065790 cites W2140018864 @default.
• W2903065790 cites W2152702121 @default.
• W2903065790 cites W2160575129 @default.
• W2903065790 cites W2162952238 @default.
• W2903065790 cites W2166420822 @default.
• W2903065790 cites W2168201480 @default.
• W2903065790 cites W2168627968 @default.
• W2903065790 cites W2284695989 @default.
• W2903065790 cites W2315662686 @default.
• W2903065790 cites W2587491863 @default.
• W2903065790 cites W2600329551 @default.
• W2903065790 cites W2610753158 @default.
• W2903065790 cites W2619806309 @default.
• W2903065790 cites W2707646777 @default.
• W2903065790 cites W2732727352 @default.
• W2903065790 cites W2745754500 @default.
• W2903065790 cites W2747992112 @default.
• W2903065790 cites W2803019104 @default.
• W2903065790 cites W4211156111 @default.
• W2903065790 cites W4245532735 @default.
• W2903065790 cites W4250598406 @default.
• W2903065790 cites W4293253343 @default.
• W2903065790 cites W598131573 @default.
• W2903065790 cites W788271370 @default.
• W2903065790 cites W794097335 @default.
• W2903065790 cites W95014119 @default.
• W2903065790 doi "https://doi.org/10.1080/19420862.2018.1546086" @default.
• W2903065790 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6512918" @default.
• W2903065790 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30513259" @default.
• W2903065790 hasPublicationYear "2018" @default.

• next