FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2903114690> ?p ?o ?g. }

• W2903114690 abstract "Myofibroblasts (MFs) are present in healthy tissues and are also key components of the tumor microenvironment. In the present study a comparative analysis of MFs obtained from various gastrointestinal tumor tissues and from tumor‑adjacent normal tissues of cancer patients was performed, with the aim to evaluate differences in MF morphology, gene expression profile and function. The goal was to correlate the observed morphological and functional variations with the underlying genetic and epigenetic backgrounds. The mutation frequency of MFs was assessed by next generation sequencing. The transcript levels of cancer‑specific genes were determined by TaqMan array and quantitative polymerase chain reaction. Epigenetic modifications were analyzed by immunocytochemistry and western blotting. The migratory capacity of MFs was assessed by scratch assay, whereas matrix metalloproteinase expression and activity were obtained by quantitative polymerase chain reaction and zymography. The results of the present study demonstrate that MFs were present in an increased number and with altered morphology in tumor samples compared with the healthy tissue. Although the detected number of mutations in tumor‑associated and normal tissue‑derived MFs did not differ markedly, shifts in the level of specific acetylated and methylated histone proteins, namely decreased levels of trimethylated H3K9 and acetylated H4K16 were demonstrated in tumor‑associated MFs. Transcript levels of several tumor‑specific genes involved in metastasis, regulation of cellular growth, apoptosis, as well as in hypoxia‑angiogenesis were altered in tumor‑derived MF cultures. Increased mRNA levels were obtained and activity of matrix metalloproteases in tumor‑derived MFs and these cells also exhibited a higher migratory capacity compared with the normal MFs. In summary, the results of the present study indicate that tumor‑associated MFs display an altered phenotype compared with healthy tissue derived counterparts. The results imply that epigenetic rather than genetic alterations are associated with the development of the distinct expressional and functional features, which define this MF phenotype in the tumor microenvironment." @default.
• W2903114690 created "2018-12-11" @default.
• W2903114690 creator A5022627454 @default.
• W2903114690 creator A5028774189 @default.
• W2903114690 creator A5029258224 @default.
• W2903114690 creator A5041490644 @default.
• W2903114690 creator A5054997401 @default.
• W2903114690 creator A5057257315 @default.
• W2903114690 creator A5076326038 @default.
• W2903114690 creator A5076372917 @default.
• W2903114690 creator A5076571987 @default.
• W2903114690 creator A5077887662 @default.
• W2903114690 creator A5082117105 @default.
• W2903114690 date "2018-12-06" @default.
• W2903114690 modified "2023-10-16" @default.
• W2903114690 title "Genetic, epigenetic and transcriptional comparison of esophagus tumor‑associated and adjacent normal myofibroblasts" @default.
• W2903114690 cites W1505584712 @default.
• W2903114690 cites W1515456817 @default.
• W2903114690 cites W1569332748 @default.
• W2903114690 cites W1588127651 @default.
• W2903114690 cites W1964508725 @default.
• W2903114690 cites W1968650422 @default.
• W2903114690 cites W1970159544 @default.
• W2903114690 cites W1980576740 @default.
• W2903114690 cites W1991370238 @default.
• W2903114690 cites W1992885568 @default.
• W2903114690 cites W2008387441 @default.
• W2903114690 cites W2011046820 @default.
• W2903114690 cites W2023965283 @default.
• W2903114690 cites W2029145883 @default.
• W2903114690 cites W2034038746 @default.
• W2903114690 cites W2041492502 @default.
• W2903114690 cites W2044835152 @default.
• W2903114690 cites W2049198267 @default.
• W2903114690 cites W2057193579 @default.
• W2903114690 cites W2061958868 @default.
• W2903114690 cites W2075077128 @default.
• W2903114690 cites W2078820501 @default.
• W2903114690 cites W2088639241 @default.
• W2903114690 cites W2089956125 @default.
• W2903114690 cites W2090784961 @default.
• W2903114690 cites W2092743032 @default.
• W2903114690 cites W2097271076 @default.
• W2903114690 cites W2102643000 @default.
• W2903114690 cites W2106798509 @default.
• W2903114690 cites W2107277218 @default.
• W2903114690 cites W2108337520 @default.
• W2903114690 cites W2117692326 @default.
• W2903114690 cites W2131584053 @default.
• W2903114690 cites W2151552433 @default.
• W2903114690 cites W2153804605 @default.
• W2903114690 cites W2164479838 @default.
• W2903114690 cites W2168631000 @default.
• W2903114690 cites W2190528488 @default.
• W2903114690 cites W2192080449 @default.
• W2903114690 cites W2263390674 @default.
• W2903114690 cites W2303423278 @default.
• W2903114690 cites W2368434880 @default.
• W2903114690 cites W2465751369 @default.
• W2903114690 cites W2470158267 @default.
• W2903114690 cites W2512899508 @default.
• W2903114690 cites W2525107653 @default.
• W2903114690 cites W2555729723 @default.
• W2903114690 cites W2569856193 @default.
• W2903114690 cites W2591628935 @default.
• W2903114690 cites W2736102792 @default.
• W2903114690 cites W2743860931 @default.
• W2903114690 cites W2751637972 @default.
• W2903114690 cites W2765528838 @default.
• W2903114690 cites W2770791015 @default.
• W2903114690 cites W2771655294 @default.
• W2903114690 cites W2792115930 @default.
• W2903114690 doi "https://doi.org/10.3892/or.2018.6909" @default.
• W2903114690 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6313073" @default.
• W2903114690 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30535493" @default.
• W2903114690 hasPublicationYear "2018" @default.
• W2903114690 type Work @default.
• W2903114690 sameAs 2903114690 @default.
• W2903114690 citedByCount "3" @default.
• W2903114690 countsByYear W29031146902020 @default.
• W2903114690 countsByYear W29031146902022 @default.
• W2903114690 crossrefType "journal-article" @default.
• W2903114690 hasAuthorship W2903114690A5022627454 @default.
• W2903114690 hasAuthorship W2903114690A5028774189 @default.
• W2903114690 hasAuthorship W2903114690A5029258224 @default.
• W2903114690 hasAuthorship W2903114690A5041490644 @default.
• W2903114690 hasAuthorship W2903114690A5054997401 @default.
• W2903114690 hasAuthorship W2903114690A5057257315 @default.
• W2903114690 hasAuthorship W2903114690A5076326038 @default.
• W2903114690 hasAuthorship W2903114690A5076372917 @default.
• W2903114690 hasAuthorship W2903114690A5076571987 @default.
• W2903114690 hasAuthorship W2903114690A5077887662 @default.
• W2903114690 hasAuthorship W2903114690A5082117105 @default.
• W2903114690 hasBestOaLocation W29031146901 @default.
• W2903114690 hasConcept C104317684 @default.
• W2903114690 hasConcept C121608353 @default.
• W2903114690 hasConcept C142724271 @default.
• W2903114690 hasConcept C153911025 @default.
• W2903114690 hasConcept C2781018059 @default.
• W2903114690 hasConcept C29537977 @default.

• next