Matches in SemOpenAlex for { <https://semopenalex.org/work/W2903224892> ?p ?o ?g. }
- W2903224892 endingPage "317" @default.
- W2903224892 startingPage "310" @default.
- W2903224892 abstract "Optimal meibomian gland (MG) function is critically important for the health and wellbeing of the ocular surface. We hypothesize that low oxygen (O2) conditions promote the function of human MG epithelial cells (HMGECs) and that human MGs exist in a relatively hypoxic environment. The purpose of this study was to test our hypotheses. We used human and mouse eyelid segments, and immortalized human MG epithelial cells (IHMGECs) in our studies. To evaluate oxygen (O2) levels in the mouse MG and vicinity, we injected pimonidazole (pimo), a hypoxia marker, before sacrifice. Human eyelid samples were stained with the hypoxia marker glucose transporter 1 (Glut-1). To determine the effect of low O2 levels on IHMGECs, we cultured cells under proliferating and differentiating conditions in both normoxic (21% O2) and hypoxic (3% O2) conditions for 5–15 days. IHMGECs were evaluated for cell number, neutral lipid content, lysosome accumulation, expression of biomarker proteins and DNase II activity. Our results demonstrate that human and mouse MGs, but not the surrounding connective tissue, exist in a relatively hypoxic environment in vivo. In addition, our findings show that hypoxia does not influence IHMGEC numbers in basal or proliferating culture conditions, but does stimulate the expression of SREBP-1 in differentiating IHMGECs. Hypoxia also significantly increased DNase II activity, and apparently IHMGEC terminal differentiation. Our Results support our hypotheses, and indicate that relative hypoxia promotes MG function." @default.
- W2903224892 created "2018-12-11" @default.
- W2903224892 creator A5028478656 @default.
- W2903224892 creator A5031574868 @default.
- W2903224892 creator A5032816799 @default.
- W2903224892 creator A5038317777 @default.
- W2903224892 creator A5046561927 @default.
- W2903224892 creator A5067130875 @default.
- W2903224892 date "2019-04-01" @default.
- W2903224892 modified "2023-10-14" @default.
- W2903224892 title "Hypoxia: A breath of fresh air for the meibomian gland" @default.
- W2903224892 cites W1507896224 @default.
- W2903224892 cites W1563822695 @default.
- W2903224892 cites W1970613833 @default.
- W2903224892 cites W1970744454 @default.
- W2903224892 cites W1972724414 @default.
- W2903224892 cites W1977550761 @default.
- W2903224892 cites W1978282308 @default.
- W2903224892 cites W1979046490 @default.
- W2903224892 cites W1986303388 @default.
- W2903224892 cites W1986422289 @default.
- W2903224892 cites W1986642304 @default.
- W2903224892 cites W1988886218 @default.
- W2903224892 cites W1993900282 @default.
- W2903224892 cites W2001553754 @default.
- W2903224892 cites W2005288369 @default.
- W2903224892 cites W2007713463 @default.
- W2903224892 cites W2008187828 @default.
- W2903224892 cites W2008993758 @default.
- W2903224892 cites W2009983804 @default.
- W2903224892 cites W2012667683 @default.
- W2903224892 cites W2014017843 @default.
- W2903224892 cites W2033803279 @default.
- W2903224892 cites W2034672829 @default.
- W2903224892 cites W2049761278 @default.
- W2903224892 cites W2050273417 @default.
- W2903224892 cites W2050870367 @default.
- W2903224892 cites W2059863732 @default.
- W2903224892 cites W2064947126 @default.
- W2903224892 cites W2071335476 @default.
- W2903224892 cites W2073890475 @default.
- W2903224892 cites W2075669327 @default.
- W2903224892 cites W2081835864 @default.
- W2903224892 cites W2082724244 @default.
- W2903224892 cites W2084037323 @default.
- W2903224892 cites W2089916471 @default.
- W2903224892 cites W2091786916 @default.
- W2903224892 cites W2092078694 @default.
- W2903224892 cites W2092943609 @default.
- W2903224892 cites W2094875875 @default.
- W2903224892 cites W2104706512 @default.
- W2903224892 cites W2108712781 @default.
- W2903224892 cites W2108924624 @default.
- W2903224892 cites W2123105623 @default.
- W2903224892 cites W2128618849 @default.
- W2903224892 cites W2136385649 @default.
- W2903224892 cites W2139606964 @default.
- W2903224892 cites W2142179552 @default.
- W2903224892 cites W2143619267 @default.
- W2903224892 cites W2146544786 @default.
- W2903224892 cites W2151607437 @default.
- W2903224892 cites W2152278396 @default.
- W2903224892 cites W2154257258 @default.
- W2903224892 cites W2155342793 @default.
- W2903224892 cites W2156023431 @default.
- W2903224892 cites W2162012682 @default.
- W2903224892 cites W2163785631 @default.
- W2903224892 cites W2285483222 @default.
- W2903224892 cites W2313816167 @default.
- W2903224892 cites W2322220244 @default.
- W2903224892 cites W2330997811 @default.
- W2903224892 cites W2340628750 @default.
- W2903224892 cites W2398108838 @default.
- W2903224892 cites W2431250996 @default.
- W2903224892 cites W2510138064 @default.
- W2903224892 cites W2535321036 @default.
- W2903224892 cites W2542943305 @default.
- W2903224892 cites W2591118509 @default.
- W2903224892 cites W2612960139 @default.
- W2903224892 cites W2692981981 @default.
- W2903224892 cites W2739359388 @default.
- W2903224892 cites W2804308128 @default.
- W2903224892 cites W2804434142 @default.
- W2903224892 cites W4252021281 @default.
- W2903224892 doi "https://doi.org/10.1016/j.jtos.2018.12.001" @default.
- W2903224892 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6529253" @default.
- W2903224892 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30528291" @default.
- W2903224892 hasPublicationYear "2019" @default.
- W2903224892 type Work @default.
- W2903224892 sameAs 2903224892 @default.
- W2903224892 citedByCount "18" @default.
- W2903224892 countsByYear W29032248922020 @default.
- W2903224892 countsByYear W29032248922021 @default.
- W2903224892 countsByYear W29032248922022 @default.
- W2903224892 countsByYear W29032248922023 @default.
- W2903224892 crossrefType "journal-article" @default.
- W2903224892 hasAuthorship W2903224892A5028478656 @default.
- W2903224892 hasAuthorship W2903224892A5031574868 @default.