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- W2903402414 abstract "Abstract Small cell lung cancer (SCLC) needs to be classified from poorly differentiated lung adenocarcinoma (PDLAC) for appropriate treatment of lung cancer patients. Currently, the classification is achieved by experienced clinicians, radiologists and pathologists based on subjective and qualitative analysis of imaging, cytological and immunohistochemical (IHC) features. Label‐free classification of lung cancer cell lines is developed here by using two‐dimensional (2D) light scattering static cytometric technique. Measurements of scattered light at forward scattering (FSC) and side scattering (SSC) by using conventional cytometry show that SCLC cells are overlapped with PDLAC cells. However, our 2D light scattering static cytometer reveals remarkable differences between the 2D light scattering patterns of SCLC cell lines (H209 and H69) and PDLAC cell line (SK‐LU‐1). By adopting support vector machine (SVM) classifier with leave‐one‐out cross‐validation (LOO‐CV), SCLC and PDLAC cells are automatically classified with an accuracy of 99.87%. Our label‐free 2D light scattering static cytometer may serve as a new, accurate, and easy‐to‐use method for the automatic classification of SCLC and PDLAC cells. © 2018 International Society for Advancement of Cytometry" @default.
- W2903402414 created "2018-12-11" @default.
- W2903402414 creator A5033206850 @default.
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- W2903402414 date "2018-12-03" @default.
- W2903402414 modified "2023-10-18" @default.
- W2903402414 title "Automatic Classification of Label‐Free Cells from Small Cell Lung Cancer and Poorly Differentiated Lung Adenocarcinoma with 2D Light Scattering Static Cytometry and Machine Learning" @default.
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- W2903402414 doi "https://doi.org/10.1002/cyto.a.23671" @default.
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