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• W2903405098 endingPage "1303" @default.
• W2903405098 startingPage "1303" @default.
• W2903405098 abstract "Background: Metastasis is the leading cause of cancer deaths. Migration of tumor cells is an important stage in metastasis. Therefore, recent studies have focused on clarifying migration and migration-dependent cell functions such as angiogenesis, wound healing, and invasion.Objectives: In the present study, we aimed to investigate the effect of acetazolamide, which is a classical carbonic anhydrase inhibitor, on the cell viability, migration, and colony forming capacity of human LS174T colorectal cancer cells. Methods: Three different cell culture techniques (MTT test, wound healing and clonogenic assay) were performed in this in vitro study on colorectal cancer cells.Results: Acetazolamide reduced the cell viability, migration and colony formation ability of cells depending on dose. There was no significant difference between the cells treated with acetazolamide with 1 μM dose and the control. However, it can be concluded that acetazolamide exerts its effect on human colorectal cancer cells at 10-1000 µM concentrations.Conclusion: Acetazolamide was observed to significantly inhibit the cell viability, colony forming capacity, and migration ability in the culture medium of LS174T cells. This inhibitor effect of acetazolamide was observed to be dependent on the concentration in medium. Keywords: LS174T, carbonic anhydrase inhibitor, colorectal carcinoma." @default.
• W2903405098 created "2018-12-11" @default.
• W2903405098 creator A5037032224 @default.
• W2903405098 creator A5039910565 @default.
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• W2903405098 date "2018-11-29" @default.
• W2903405098 modified "2023-10-16" @default.
• W2903405098 title "Inhibition of cell proliferation, migration and colony formation of LS174T Cells by carbonic anhydrase inhibitor" @default.
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• W2903405098 doi "https://doi.org/10.4314/ahs.v18i4.51" @default.
• W2903405098 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6354875" @default.
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• W2903405098 hasPublicationYear "2018" @default.
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