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- W2903600265 startingPage "209" @default.
- W2903600265 abstract "The high metabolic activity to which the dairy cattle are exposed to maintain milk production altered steroid metabolism that affects reproductive physiology and reduce oocyte competence. Our aims were (a) to characterize the competence of immature oocytes collected from dairy cattle based on the expression of genes in cumulus cells (CCs) and (b) to improve oocyte competence to support preimplantation embryo development by the supplementation of maturation medium with bone morphogenetic protein 15 (BMP15) and/or anti‐mullerian hormone (AMH). Oocyte donors were identified at the moment of ovary collection and grouped by involuntarily culled dairy cows (Holstein breed) or beef cattle. The embryo development speed to blastocyst of the cull dairy cattle versus beef cattle (control group) was lower. Besides, <10% of oocytes (with CC biopsies) derived from dairy cattle were able to develop to the blastocyst stage. In addition, a higher level of expression and a positive correlation were observed in the expression of most of the genes evaluated (LUM, KRT18, KRT8, CLIC3, BMPR1B, and SLC38A3) in the cumulus–oocyte complexes that produced blastocysts versus those which did not develop correctly (arrested development). Further, use of BMP15 in the maturation of oocytes from dairy cattle seems to increase competence, modulating the expression of OCT4, SOX2, CDX2, GATA6, and TP1 in resulting blastocysts." @default.
- W2903600265 created "2018-12-22" @default.
- W2903600265 creator A5038175554 @default.
- W2903600265 creator A5051472731 @default.
- W2903600265 creator A5058480476 @default.
- W2903600265 creator A5068013396 @default.
- W2903600265 date "2018-12-27" @default.
- W2903600265 modified "2023-09-25" @default.
- W2903600265 title "Effect of BMP15 and/or AMH during in vitro maturation of oocytes from involuntarily culled dairy cows" @default.
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