Matches in SemOpenAlex for { <https://semopenalex.org/work/W2903601047> ?p ?o ?g. }
- W2903601047 endingPage "460" @default.
- W2903601047 startingPage "452" @default.
- W2903601047 abstract "The milk thistle compound Silibinin (i.e., a 1:1 mixture of Silybin A and Silybin B) stimulates vasculogenesis of mouse embryonic stem (ES) cells. Because vasculogenesis and leukopoiesis are interrelated, the effect of Silibinin on leukopoiesis of ES cells was investigated. Treatment of differentiating ES cells with hydrosoluble Silibinin-C-2',3-dihydrogen succinate dose-dependent increased the number of CD18+ , CD45+ , and CD68+ cells, indicating leukocyte/macrophage differentiation. Silibinin treatment activated phosphoinositide 3-kinase (PI3K), AKT (protein kinase B), signal transducer and activator of transcription 3 (STAT3), stimulated hypoxia-induced factor-1α (HIF-1α), and vascular endothelial growth factor receptor 2 (VEGFR2) expression and raised intracellular nitric oxide (NO). Western blot experiments showed that upon coincubation with either the PI3K inhibitor LY294002, the STAT3 inhibitor Stattic, the AKT antagonist AKT inhibitor VIII, or the NO inhibitor L-NAME, the Silibinin-induced expression of CD18, CD45, and CD68 was abolished. Moreover, the stimulation of HIF-1α and VEGFR2 expression was blunted upon STAT3 and PI3K/AKT inhibition. Treatment of differentiating ES cells with L-NAME abolished the stimulation of VEGFR2 and VE-cadherin expression achieved with Silibinin, indicating that NO is involved in vasculogenesis and leukocyte differentiation pathways. In summary, the data of the present study demonstrate that Silibinin stimulates leukocyte differentiation of ES cells, which is associated to vasculogenesis and regulated by PI3K/AKT-, STAT3-, and NO-mediated signaling." @default.
- W2903601047 created "2018-12-22" @default.
- W2903601047 creator A5021490438 @default.
- W2903601047 creator A5035353308 @default.
- W2903601047 creator A5045881557 @default.
- W2903601047 creator A5080937883 @default.
- W2903601047 date "2018-12-11" @default.
- W2903601047 modified "2023-10-09" @default.
- W2903601047 title "The milk thistle (Silybum marianum)compound Silibinin stimulates leukopoiesis from mouse embryonic stem cells" @default.
- W2903601047 cites W119147594 @default.
- W2903601047 cites W1873852079 @default.
- W2903601047 cites W1950745665 @default.
- W2903601047 cites W195660419 @default.
- W2903601047 cites W1964491823 @default.
- W2903601047 cites W1965192138 @default.
- W2903601047 cites W1970398864 @default.
- W2903601047 cites W1976950210 @default.
- W2903601047 cites W1979215811 @default.
- W2903601047 cites W1988223087 @default.
- W2903601047 cites W1991582729 @default.
- W2903601047 cites W2000868542 @default.
- W2903601047 cites W2009673421 @default.
- W2903601047 cites W2011108652 @default.
- W2903601047 cites W2041168245 @default.
- W2903601047 cites W2047969330 @default.
- W2903601047 cites W2053272006 @default.
- W2903601047 cites W2066767319 @default.
- W2903601047 cites W2072566300 @default.
- W2903601047 cites W2101025114 @default.
- W2903601047 cites W2117890432 @default.
- W2903601047 cites W2118995216 @default.
- W2903601047 cites W2120386458 @default.
- W2903601047 cites W2133860937 @default.
- W2903601047 cites W2142110193 @default.
- W2903601047 cites W2164746176 @default.
- W2903601047 cites W2166205453 @default.
- W2903601047 cites W2167279371 @default.
- W2903601047 cites W2171611058 @default.
- W2903601047 cites W2325863196 @default.
- W2903601047 cites W2345774905 @default.
- W2903601047 cites W2402015540 @default.
- W2903601047 cites W2463620236 @default.
- W2903601047 cites W2469619401 @default.
- W2903601047 cites W2477133542 @default.
- W2903601047 cites W2505213728 @default.
- W2903601047 cites W2580291574 @default.
- W2903601047 cites W2615103644 @default.
- W2903601047 cites W2733433808 @default.
- W2903601047 cites W2769701504 @default.
- W2903601047 cites W2778876317 @default.
- W2903601047 cites W2782959532 @default.
- W2903601047 cites W2783427075 @default.
- W2903601047 cites W2783935176 @default.
- W2903601047 cites W2785926425 @default.
- W2903601047 cites W2886246189 @default.
- W2903601047 doi "https://doi.org/10.1002/ptr.6241" @default.
- W2903601047 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30548344" @default.
- W2903601047 hasPublicationYear "2018" @default.
- W2903601047 type Work @default.
- W2903601047 sameAs 2903601047 @default.
- W2903601047 citedByCount "3" @default.
- W2903601047 countsByYear W29036010472020 @default.
- W2903601047 countsByYear W29036010472023 @default.
- W2903601047 crossrefType "journal-article" @default.
- W2903601047 hasAuthorship W2903601047A5021490438 @default.
- W2903601047 hasAuthorship W2903601047A5035353308 @default.
- W2903601047 hasAuthorship W2903601047A5045881557 @default.
- W2903601047 hasAuthorship W2903601047A5080937883 @default.
- W2903601047 hasConcept C153911025 @default.
- W2903601047 hasConcept C185592680 @default.
- W2903601047 hasConcept C201750760 @default.
- W2903601047 hasConcept C2776912716 @default.
- W2903601047 hasConcept C2777130413 @default.
- W2903601047 hasConcept C2780210117 @default.
- W2903601047 hasConcept C28328180 @default.
- W2903601047 hasConcept C2909423643 @default.
- W2903601047 hasConcept C502942594 @default.
- W2903601047 hasConcept C59822182 @default.
- W2903601047 hasConcept C60781801 @default.
- W2903601047 hasConcept C62478195 @default.
- W2903601047 hasConcept C75217442 @default.
- W2903601047 hasConcept C86554907 @default.
- W2903601047 hasConcept C86803240 @default.
- W2903601047 hasConcept C95444343 @default.
- W2903601047 hasConcept C98274493 @default.
- W2903601047 hasConceptScore W2903601047C153911025 @default.
- W2903601047 hasConceptScore W2903601047C185592680 @default.
- W2903601047 hasConceptScore W2903601047C201750760 @default.
- W2903601047 hasConceptScore W2903601047C2776912716 @default.
- W2903601047 hasConceptScore W2903601047C2777130413 @default.
- W2903601047 hasConceptScore W2903601047C2780210117 @default.
- W2903601047 hasConceptScore W2903601047C28328180 @default.
- W2903601047 hasConceptScore W2903601047C2909423643 @default.
- W2903601047 hasConceptScore W2903601047C502942594 @default.
- W2903601047 hasConceptScore W2903601047C59822182 @default.
- W2903601047 hasConceptScore W2903601047C60781801 @default.
- W2903601047 hasConceptScore W2903601047C62478195 @default.
- W2903601047 hasConceptScore W2903601047C75217442 @default.