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• W2903851615 endingPage "e1007481" @default.
• W2903851615 startingPage "e1007481" @default.
• W2903851615 abstract "Cytomegaloviruses (CMVs) have a highly restricted host range as they replicate only in cells of their own or closely related species. To date, the molecular mechanisms underlying the CMV host restriction remain poorly understood. However, it has been shown that mouse cytomegalovirus (MCMV) can be adapted to human cells and that adaptation goes along with adaptive mutations in several viral genes. In this study, we identify MCMV M117 as a novel host range determinant. Mutations in this gene enable the virus to cross the species barrier and replicate in human RPE-1 cells. We show that the M117 protein is expressed with early kinetics, localizes to viral replication compartments, and contributes to the inhibition of cellular DNA synthesis. Mechanistically, M117 interacts with members of the E2F transcription factor family and induces E2F target gene expression in murine and human cells. While the N-terminal part of M117 mediates E2F interaction, the C-terminal part mediates self-interaction. Both parts are required for the activation of E2F-dependent transcription. We further show that M117 is dispensable for viral replication in cultured mouse fibroblasts and endothelial cells, but is required for colonization of mouse salivary glands in vivo. Conversely, inactivation of M117 or pharmacological inhibition of E2F facilitates MCMV replication in human RPE-1 cells, whereas replacement of M117 by adenovirus E4orf6/7, a known E2F activator, prevents it. These results indicate that E2F activation is detrimental for MCMV replication in human cells. In summary, this study identifies MCMV M117 as a novel E2F activator that functions as a host range determinant by precluding MCMV replication in human cells." @default.
• W2903851615 created "2018-12-22" @default.
• W2903851615 creator A5004448432 @default.
• W2903851615 creator A5023752156 @default.
• W2903851615 creator A5035734503 @default.
• W2903851615 creator A5063858118 @default.
• W2903851615 creator A5072725079 @default.
• W2903851615 creator A5083292913 @default.
• W2903851615 date "2018-12-10" @default.
• W2903851615 modified "2023-10-01" @default.
• W2903851615 title "Activation of E2F-dependent transcription by the mouse cytomegalovirus M117 protein affects the viral host range" @default.
• W2903851615 cites W1601653727 @default.
• W2903851615 cites W1614146403 @default.
• W2903851615 cites W1685950740 @default.
• W2903851615 cites W179475632 @default.
• W2903851615 cites W1816486083 @default.
• W2903851615 cites W1851333263 @default.
• W2903851615 cites W1870943906 @default.
• W2903851615 cites W1933762305 @default.
• W2903851615 cites W1943039657 @default.
• W2903851615 cites W1967652534 @default.
• W2903851615 cites W1974484718 @default.
• W2903851615 cites W1980807416 @default.
• W2903851615 cites W1980834271 @default.
• W2903851615 cites W1986555537 @default.
• W2903851615 cites W1989422196 @default.
• W2903851615 cites W1991199800 @default.
• W2903851615 cites W1992802797 @default.
• W2903851615 cites W1995146190 @default.
• W2903851615 cites W1995482329 @default.
• W2903851615 cites W2008960254 @default.
• W2903851615 cites W2010367357 @default.
• W2903851615 cites W2015003540 @default.
• W2903851615 cites W2031967690 @default.
• W2903851615 cites W2033028600 @default.
• W2903851615 cites W2034767282 @default.
• W2903851615 cites W2047698252 @default.
• W2903851615 cites W2049356309 @default.
• W2903851615 cites W2053146635 @default.
• W2903851615 cites W2054408541 @default.
• W2903851615 cites W2057553119 @default.
• W2903851615 cites W2057563271 @default.
• W2903851615 cites W2065343028 @default.
• W2903851615 cites W2067290870 @default.
• W2903851615 cites W2068153306 @default.
• W2903851615 cites W2069023071 @default.
• W2903851615 cites W2069717463 @default.
• W2903851615 cites W2074920283 @default.
• W2903851615 cites W2078911429 @default.
• W2903851615 cites W2081814637 @default.
• W2903851615 cites W2083187335 @default.
• W2903851615 cites W2088322442 @default.
• W2903851615 cites W2095778156 @default.
• W2903851615 cites W2097220925 @default.
• W2903851615 cites W2097691794 @default.
• W2903851615 cites W2108367007 @default.
• W2903851615 cites W2110429239 @default.
• W2903851615 cites W2115834887 @default.
• W2903851615 cites W2118667156 @default.
• W2903851615 cites W2120145798 @default.
• W2903851615 cites W2124716540 @default.
• W2903851615 cites W2125951714 @default.
• W2903851615 cites W2127260871 @default.
• W2903851615 cites W2131308181 @default.
• W2903851615 cites W2131675463 @default.
• W2903851615 cites W2131777296 @default.
• W2903851615 cites W2132786809 @default.
• W2903851615 cites W2135182392 @default.
• W2903851615 cites W2140807899 @default.
• W2903851615 cites W2146218933 @default.
• W2903851615 cites W2147611204 @default.
• W2903851615 cites W2148382823 @default.
• W2903851615 cites W2148850786 @default.
• W2903851615 cites W2149012701 @default.
• W2903851615 cites W2150366724 @default.
• W2903851615 cites W2152501564 @default.
• W2903851615 cites W2152951165 @default.
• W2903851615 cites W2153565075 @default.
• W2903851615 cites W2154281042 @default.
• W2903851615 cites W2156172405 @default.
• W2903851615 cites W2157303629 @default.
• W2903851615 cites W2160321820 @default.
• W2903851615 cites W2160542375 @default.
• W2903851615 cites W2161086529 @default.
• W2903851615 cites W2164654743 @default.
• W2903851615 cites W2169129743 @default.
• W2903851615 cites W2291798274 @default.
• W2903851615 cites W2345841092 @default.
• W2903851615 cites W2415423535 @default.
• W2903851615 cites W2430533583 @default.
• W2903851615 cites W2591801412 @default.
• W2903851615 cites W2595469715 @default.
• W2903851615 cites W2791873134 @default.
• W2903851615 cites W2794921707 @default.
• W2903851615 doi "https://doi.org/10.1371/journal.ppat.1007481" @default.
• W2903851615 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6301716" @default.
• W2903851615 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30532172" @default.
• W2903851615 hasPublicationYear "2018" @default.

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