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• W2903978922 abstract "Genetic manipulation is a fundamental procedure for the study of gene and operon functions and new characteristics acquisition. Modern CRISPR-Cas technology allows genome editing more precisely and increases the efficiency of transferring mutations in a variety of hard to manipulate organisms. Here, we describe new CRISPR-Cas vectors for genetic modifications in bacillary species. Our plasmids are single CRISPR-Cas plasmids comprising all components for genome editing and should be functional in a broad host range. They are highly efficient (up to 97%) and precise. The employment and delivery of these plasmids to bacillary strains can be easily achieved by conjugation from Escherichia coli. During our research we also demonstrated the absence of compatibility between CRISPR-Cas system and non-homologous end joining in Bacillus subtilis." @default.
• W2903978922 created "2018-12-22" @default.
• W2903978922 creator A5030404503 @default.
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• W2903978922 date "2018-12-06" @default.
• W2903978922 modified "2023-10-11" @default.
• W2903978922 title "New CRISPR-Cas9 vectors for genetic modifications of<i>Bacillus</i>species" @default.
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• W2903978922 doi "https://doi.org/10.1093/femsle/fny284" @default.
• W2903978922 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30520985" @default.
• W2903978922 hasPublicationYear "2018" @default.
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