FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2904004929> ?p ?o ?g. }

• W2904004929 endingPage "1552059" @default.
• W2904004929 startingPage "1552059" @default.
• W2904004929 abstract "Foetal calf serum (FCS) is a common supplement of cell culture medium and a known source of contaminating extracellular vesicles (EV) containing RNA. Because of a high degree of sequence similarity among homologous non-coding RNAs of mammalian species, residual FCS-RNA in culture medium may interfere in the analysis of EV-RNA released by cultured cells. Recently, doubts have been raised as to whether commonly used protocols for depletion of FCS-EV efficiently remove FCS-RNA. Moreover, technical details in FCS-EV depletion protocols are known to vary between labs, which may lead to inter-study differences in contaminating FCS-RNA levels. Here, we investigated how technical modifications of EV-depletion protocols affect the efficiency with which bovine RNAs are depleted from FCS, and determined the contribution of contaminating bovine RNA to EV-RNA purified from cell cultures. Our data show differences in depletion efficiency between and within various classes of small non-coding RNA. Importantly, we demonstrate that variations in FCS-EV depletion protocols affect both the quantity and type of residual FCS-RNAs in EV-depleted medium. By using optimised FCS-EV depletion protocols combined with methods for high-grade purification of EV the levels of contaminating bovine RNA in EV populations isolated from cell culture medium can be reduced. With illustrative datasets we also demonstrate that the abundance of a specific RNA in cell culture EV can only be determined if measured relative to background levels of this RNA in medium control samples. These data highlight the need for optimisation and validation of existing and novel FCS-EV depletion methods and urge for accurate descriptions of these methods in publications to increase experimental reproducibility." @default.
• W2904004929 created "2018-12-22" @default.
• W2904004929 creator A5051288617 @default.
• W2904004929 creator A5055254489 @default.
• W2904004929 creator A5087699615 @default.
• W2904004929 date "2018-12-12" @default.
• W2904004929 modified "2023-10-17" @default.
• W2904004929 title "Technical approaches to reduce interference of Fetal calf serum derived RNA in the analysis of extracellular vesicle RNA from cultured cells" @default.
• W2904004929 cites W1513539546 @default.
• W2904004929 cites W1793480972 @default.
• W2904004929 cites W1975320047 @default.
• W2904004929 cites W1990820461 @default.
• W2904004929 cites W1994809451 @default.
• W2904004929 cites W1996301440 @default.
• W2904004929 cites W1996600340 @default.
• W2904004929 cites W2004895989 @default.
• W2904004929 cites W2005381457 @default.
• W2904004929 cites W2006986124 @default.
• W2904004929 cites W2024206172 @default.
• W2904004929 cites W2032696674 @default.
• W2904004929 cites W2039731107 @default.
• W2904004929 cites W2093013992 @default.
• W2904004929 cites W2093676674 @default.
• W2904004929 cites W2107277218 @default.
• W2904004929 cites W2116572568 @default.
• W2904004929 cites W2128384671 @default.
• W2904004929 cites W2154479094 @default.
• W2904004929 cites W2159401023 @default.
• W2904004929 cites W2167591654 @default.
• W2904004929 cites W2167604259 @default.
• W2904004929 cites W2192080449 @default.
• W2904004929 cites W2287188530 @default.
• W2904004929 cites W2511380210 @default.
• W2904004929 cites W2585739546 @default.
• W2904004929 cites W2593629244 @default.
• W2904004929 cites W2594599876 @default.
• W2904004929 cites W2743511563 @default.
• W2904004929 cites W2789802139 @default.
• W2904004929 cites W2805547508 @default.
• W2904004929 cites W2805918213 @default.
• W2904004929 cites W2808496870 @default.
• W2904004929 doi "https://doi.org/10.1080/20013078.2018.1552059" @default.
• W2904004929 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6292350" @default.
• W2904004929 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30559953" @default.
• W2904004929 hasPublicationYear "2018" @default.
• W2904004929 type Work @default.
• W2904004929 sameAs 2904004929 @default.
• W2904004929 citedByCount "23" @default.
• W2904004929 countsByYear W29040049292019 @default.
• W2904004929 countsByYear W29040049292020 @default.
• W2904004929 countsByYear W29040049292021 @default.
• W2904004929 countsByYear W29040049292022 @default.
• W2904004929 countsByYear W29040049292023 @default.
• W2904004929 crossrefType "journal-article" @default.
• W2904004929 hasAuthorship W2904004929A5051288617 @default.
• W2904004929 hasAuthorship W2904004929A5055254489 @default.
• W2904004929 hasAuthorship W2904004929A5087699615 @default.
• W2904004929 hasBestOaLocation W29040049291 @default.
• W2904004929 hasConcept C104317684 @default.
• W2904004929 hasConcept C112190416 @default.
• W2904004929 hasConcept C145059251 @default.
• W2904004929 hasConcept C1491633281 @default.
• W2904004929 hasConcept C153911025 @default.
• W2904004929 hasConcept C194993378 @default.
• W2904004929 hasConcept C20518536 @default.
• W2904004929 hasConcept C2908689518 @default.
• W2904004929 hasConcept C54355233 @default.
• W2904004929 hasConcept C55493867 @default.
• W2904004929 hasConcept C67705224 @default.
• W2904004929 hasConcept C81885089 @default.
• W2904004929 hasConcept C86803240 @default.
• W2904004929 hasConcept C95444343 @default.
• W2904004929 hasConceptScore W2904004929C104317684 @default.
• W2904004929 hasConceptScore W2904004929C112190416 @default.
• W2904004929 hasConceptScore W2904004929C145059251 @default.
• W2904004929 hasConceptScore W2904004929C1491633281 @default.
• W2904004929 hasConceptScore W2904004929C153911025 @default.
• W2904004929 hasConceptScore W2904004929C194993378 @default.
• W2904004929 hasConceptScore W2904004929C20518536 @default.
• W2904004929 hasConceptScore W2904004929C2908689518 @default.
• W2904004929 hasConceptScore W2904004929C54355233 @default.
• W2904004929 hasConceptScore W2904004929C55493867 @default.
• W2904004929 hasConceptScore W2904004929C67705224 @default.
• W2904004929 hasConceptScore W2904004929C81885089 @default.
• W2904004929 hasConceptScore W2904004929C86803240 @default.
• W2904004929 hasConceptScore W2904004929C95444343 @default.
• W2904004929 hasFunder F4320338335 @default.
• W2904004929 hasIssue "1" @default.
• W2904004929 hasLocation W29040049291 @default.
• W2904004929 hasLocation W29040049292 @default.
• W2904004929 hasLocation W29040049293 @default.
• W2904004929 hasLocation W29040049294 @default.
• W2904004929 hasLocation W29040049295 @default.
• W2904004929 hasOpenAccess W2904004929 @default.
• W2904004929 hasPrimaryLocation W29040049291 @default.
• W2904004929 hasRelatedWork W1807400738 @default.
• W2904004929 hasRelatedWork W202038478 @default.
• W2904004929 hasRelatedWork W2147993839 @default.

• next