Matches in SemOpenAlex for { <https://semopenalex.org/work/W2904098710> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2904098710 abstract "In recent years, vegetable oils were reported to be contaminated by mycotoxins. At the same time, demand for vegetable oils has been increasing due to its health benefit and also for its direct use or as ingredients in food. The objectives of this study were to develop and validate the QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) extraction and clean-up procedures for mycotoxins in vegetable oil by liquid chromatography tandem mass spectrometry (LC-MS/MS) and to verify the established method in vegetable oils in Malaysian market. In this study, a new, fast, cost efficient, sensitive and accurate assay method was developed, optimized and validated for the determination of aflatoxins (AFB1, AFB2, AFG1 and AFG2), ochratoxin A (OTA), zearalenone (ZEA) and deoxynivalenol (DON) in vegetable oil by a modified QuEChERS approach, which was conventionally developed for pesticides analysis. A quick procedure of mycotoxins extraction and clean-up was achieved in two steps. Firstly, target analytes were extracted into the optimized solvent acetonitrile and followed by dispersive solid phase extraction (dSPE) clean up with the optimized sorbents to remove the co-extracts. Three types of d-SPE sorbents; C18, primary secondary amine (PSA) and graphitized carbon black (GCB) and four combination ratios of two selected d-SPEs were evaluated to achieve an optimum and acceptable result. The maximum co-extracts were removed when d-SPE C18 and GCB were used at ratio 3:1 with MgSO4 anhydrous followed by LC-MS/MS analysis, using an electro spray-ionization interface (ESI). Methanol as an organic phase and formic acid (0.1%) with 5 mM ammonium acetate as a polar phase, with total run of 16 minutes and flow rate 300 µLmin-1 were applied. The validation study indicated the seven mycotoxins could be detected in the concentration range of 0.01 ngg-1 to650.17ngg-1 with correlation coefficient > 0.98. The limit of quantitation (LOQ) ranges from 0.04 ngg-1 to 2000 ngg-1. The recoveries were in the range of 87.9% to 106.6%. The repeatability and reproducibility of the analysis were within the acceptable level (0.5 – 1.3) for precision using HORRAT ratio. Significant matrix effect was compensated using the matrix matched calibration curves. After validation, the method was successfully applied to 25 vegetable oil samples including corn oil, palm oil and sunflower oil. ZEA, AFG2, AFG1 and AFB1 were among the detected mycotoxins. A new fast, cost efficient, sensitive and accurate assay method was successfully developed, validated and applied for the determination of seven mycotoxins in vegetable oils." @default.
- W2904098710 created "2018-12-22" @default.
- W2904098710 creator A5030163849 @default.
- W2904098710 date "2015-12-01" @default.
- W2904098710 modified "2023-09-26" @default.
- W2904098710 title "Method development for determination of multi-mycotoxin in vegetable oil using QuEChERS technique and liquid chromatography tandem mass spectrometry" @default.
- W2904098710 hasPublicationYear "2015" @default.
- W2904098710 type Work @default.
- W2904098710 sameAs 2904098710 @default.
- W2904098710 citedByCount "0" @default.
- W2904098710 crossrefType "dissertation" @default.
- W2904098710 hasAuthorship W2904098710A5030163849 @default.
- W2904098710 hasConcept C106848363 @default.
- W2904098710 hasConcept C161176658 @default.
- W2904098710 hasConcept C162356407 @default.
- W2904098710 hasConcept C185592680 @default.
- W2904098710 hasConcept C2775926907 @default.
- W2904098710 hasConcept C2776008721 @default.
- W2904098710 hasConcept C2777140777 @default.
- W2904098710 hasConcept C2778467254 @default.
- W2904098710 hasConcept C2779096019 @default.
- W2904098710 hasConcept C31903555 @default.
- W2904098710 hasConcept C40758303 @default.
- W2904098710 hasConcept C43617362 @default.
- W2904098710 hasConcept C4725764 @default.
- W2904098710 hasConcept C64489805 @default.
- W2904098710 hasConcept C6557445 @default.
- W2904098710 hasConcept C81426234 @default.
- W2904098710 hasConcept C84699730 @default.
- W2904098710 hasConcept C86803240 @default.
- W2904098710 hasConceptScore W2904098710C106848363 @default.
- W2904098710 hasConceptScore W2904098710C161176658 @default.
- W2904098710 hasConceptScore W2904098710C162356407 @default.
- W2904098710 hasConceptScore W2904098710C185592680 @default.
- W2904098710 hasConceptScore W2904098710C2775926907 @default.
- W2904098710 hasConceptScore W2904098710C2776008721 @default.
- W2904098710 hasConceptScore W2904098710C2777140777 @default.
- W2904098710 hasConceptScore W2904098710C2778467254 @default.
- W2904098710 hasConceptScore W2904098710C2779096019 @default.
- W2904098710 hasConceptScore W2904098710C31903555 @default.
- W2904098710 hasConceptScore W2904098710C40758303 @default.
- W2904098710 hasConceptScore W2904098710C43617362 @default.
- W2904098710 hasConceptScore W2904098710C4725764 @default.
- W2904098710 hasConceptScore W2904098710C64489805 @default.
- W2904098710 hasConceptScore W2904098710C6557445 @default.
- W2904098710 hasConceptScore W2904098710C81426234 @default.
- W2904098710 hasConceptScore W2904098710C84699730 @default.
- W2904098710 hasConceptScore W2904098710C86803240 @default.
- W2904098710 hasLocation W29040987101 @default.
- W2904098710 hasOpenAccess W2904098710 @default.
- W2904098710 hasPrimaryLocation W29040987101 @default.
- W2904098710 hasRelatedWork W1867207363 @default.
- W2904098710 hasRelatedWork W1977539087 @default.
- W2904098710 hasRelatedWork W1984683458 @default.
- W2904098710 hasRelatedWork W1991842742 @default.
- W2904098710 hasRelatedWork W2022566727 @default.
- W2904098710 hasRelatedWork W2041666305 @default.
- W2904098710 hasRelatedWork W2056861780 @default.
- W2904098710 hasRelatedWork W2170600988 @default.
- W2904098710 hasRelatedWork W2306335838 @default.
- W2904098710 hasRelatedWork W2344731206 @default.
- W2904098710 hasRelatedWork W2466352910 @default.
- W2904098710 hasRelatedWork W2594820768 @default.
- W2904098710 hasRelatedWork W2595070758 @default.
- W2904098710 hasRelatedWork W2744463632 @default.
- W2904098710 hasRelatedWork W2897038911 @default.
- W2904098710 hasRelatedWork W2903739060 @default.
- W2904098710 hasRelatedWork W3027776484 @default.
- W2904098710 hasRelatedWork W3095160693 @default.
- W2904098710 hasRelatedWork W3112443180 @default.
- W2904098710 hasRelatedWork W582456431 @default.
- W2904098710 isParatext "false" @default.
- W2904098710 isRetracted "false" @default.
- W2904098710 magId "2904098710" @default.
- W2904098710 workType "dissertation" @default.