FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2904157090> ?p ?o ?g. }

• W2904157090 endingPage "948" @default.
• W2904157090 startingPage "940" @default.
• W2904157090 abstract "Abstract Plant cryptochromes (CRYs) are blue‐light receptors that regulate light‐dependent growth, development, and circadian rhythms. A flavin adenine dinucleotide (FAD) cofactor is bound to the photolyase homology region (PHR) of plant CRYs and can be photoreduced to a neutral radical state under blue light. This photoreaction can trigger subsequent signal transduction. Plant CRYs can also bind an ATP molecule adjacent to FAD in a pocket of the PHR. Chlamydomonas reinhardtii contains a single plant CRY, named Chlamydomonas photolyase homologue 1 (CPH1). In CPH1, Cys392 and Asp393 are located near the FAD cofactor. Here we have shown that replacing Cys392 with Ser has little effect on the properties of CPH1. The C392N mutant, however, showed a faster photoreduction rate than wild‐type CPH1, together with a significantly lower oxidation rate of the neutral radical state. Substituting an Asn residue for Asp393 in CPH1 improved the binding affinity for FAD as well as the stability of the neutral radical, but photoreduction in the case of this mutant was severely inhibited. In the presence of ATP, CPH1 and its mutants exhibited significantly higher binding affinity for FAD and slower oxidation of the neutral radical. These results reveal that the residues at site 392 and the presence of ATP can tune the stability of the neutral radical, that the Asp residue at site 393 is crucial for photoreduction, and that the photoreduction rate is not determined merely by the stability of the neutral radical in CPH1." @default.
• W2904157090 created "2018-12-22" @default.
• W2904157090 creator A5002388018 @default.
• W2904157090 creator A5005729398 @default.
• W2904157090 creator A5047630214 @default.
• W2904157090 creator A5051061505 @default.
• W2904157090 creator A5052759717 @default.
• W2904157090 creator A5072651380 @default.
• W2904157090 creator A5080577523 @default.
• W2904157090 creator A5091663009 @default.
• W2904157090 date "2019-02-21" @default.
• W2904157090 modified "2023-10-15" @default.
• W2904157090 title "Impacts of Cys392, Asp393, and ATP on the FAD Binding, Photoreduction, and the Stability of the Radical State of <i>Chlamydomonas reinhardtii</i> Cryptochrome" @default.
• W2904157090 cites W1511958231 @default.
• W2904157090 cites W1551691749 @default.
• W2904157090 cites W1844128776 @default.
• W2904157090 cites W1971799901 @default.
• W2904157090 cites W1976310515 @default.
• W2904157090 cites W1981529935 @default.
• W2904157090 cites W1982159185 @default.
• W2904157090 cites W1982573383 @default.
• W2904157090 cites W1986461200 @default.
• W2904157090 cites W1992796743 @default.
• W2904157090 cites W1995296611 @default.
• W2904157090 cites W2006153112 @default.
• W2904157090 cites W2015524724 @default.
• W2904157090 cites W2022391649 @default.
• W2904157090 cites W2022996288 @default.
• W2904157090 cites W2024349939 @default.
• W2904157090 cites W2030074717 @default.
• W2904157090 cites W2031488163 @default.
• W2904157090 cites W2034328848 @default.
• W2904157090 cites W2045146940 @default.
• W2904157090 cites W2045500321 @default.
• W2904157090 cites W2049044025 @default.
• W2904157090 cites W2050141401 @default.
• W2904157090 cites W2056732935 @default.
• W2904157090 cites W2063580928 @default.
• W2904157090 cites W2065672538 @default.
• W2904157090 cites W2066494035 @default.
• W2904157090 cites W2071642624 @default.
• W2904157090 cites W2077619078 @default.
• W2904157090 cites W2078931626 @default.
• W2904157090 cites W2082613359 @default.
• W2904157090 cites W2083263204 @default.
• W2904157090 cites W2083521907 @default.
• W2904157090 cites W2093216727 @default.
• W2904157090 cites W2110810199 @default.
• W2904157090 cites W2113944910 @default.
• W2904157090 cites W2117719962 @default.
• W2904157090 cites W2118482425 @default.
• W2904157090 cites W2126542250 @default.
• W2904157090 cites W2132926880 @default.
• W2904157090 cites W2134736771 @default.
• W2904157090 cites W2139958072 @default.
• W2904157090 cites W2153834186 @default.
• W2904157090 cites W2161007056 @default.
• W2904157090 cites W2164610115 @default.
• W2904157090 cites W2167417236 @default.
• W2904157090 cites W2257280499 @default.
• W2904157090 cites W2258584056 @default.
• W2904157090 cites W2307486321 @default.
• W2904157090 cites W2311203695 @default.
• W2904157090 cites W2607320723 @default.
• W2904157090 cites W2613965180 @default.
• W2904157090 cites W2804822363 @default.
• W2904157090 cites W4231406401 @default.
• W2904157090 doi "https://doi.org/10.1002/cbic.201800660" @default.
• W2904157090 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30548754" @default.
• W2904157090 hasPublicationYear "2019" @default.
• W2904157090 type Work @default.
• W2904157090 sameAs 2904157090 @default.
• W2904157090 citedByCount "4" @default.
• W2904157090 countsByYear W29041570902020 @default.
• W2904157090 countsByYear W29041570902021 @default.
• W2904157090 crossrefType "journal-article" @default.
• W2904157090 hasAuthorship W2904157090A5002388018 @default.
• W2904157090 hasAuthorship W2904157090A5005729398 @default.
• W2904157090 hasAuthorship W2904157090A5047630214 @default.
• W2904157090 hasAuthorship W2904157090A5051061505 @default.
• W2904157090 hasAuthorship W2904157090A5052759717 @default.
• W2904157090 hasAuthorship W2904157090A5072651380 @default.
• W2904157090 hasAuthorship W2904157090A5080577523 @default.
• W2904157090 hasAuthorship W2904157090A5091663009 @default.
• W2904157090 hasConcept C104317684 @default.
• W2904157090 hasConcept C12554922 @default.
• W2904157090 hasConcept C134935766 @default.
• W2904157090 hasConcept C143065580 @default.
• W2904157090 hasConcept C144543706 @default.
• W2904157090 hasConcept C165204475 @default.
• W2904157090 hasConcept C181199279 @default.
• W2904157090 hasConcept C185592680 @default.
• W2904157090 hasConcept C197957613 @default.
• W2904157090 hasConcept C2775859393 @default.
• W2904157090 hasConcept C2776580309 @default.
• W2904157090 hasConcept C2777727519 @default.
• W2904157090 hasConcept C33093398 @default.
• W2904157090 hasConcept C38606739 @default.

• next