FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2904176836> ?p ?o ?g. }

• W2904176836 endingPage "42" @default.
• W2904176836 startingPage "37" @default.
• W2904176836 abstract "Vibrio parahaemolyticus is one of the important foodborne pathogens is of public health concern due to the emergence of pandemic strains causing disease outbreaks worldwide. We evaluated the DNA based colony hybridization technique for the detection and enumeration of total and pathogenic V. parahaemolyticus from the bivalve shellfish, clam using non-radioactive, enzyme-labeled probe targeting the tlh and trh genes, respectively. The digoxigenin (DIG) labeled probes designed in this study showed 100% specificity by dot blot assay. Colony hybridization using DIG probes was performed using both non-selective, trypticase soy agar (TSA) and the selective medium, thiosulfate citrate bile salts sucrose (TCBS) agar. Of 32 clam samples analyzed, 71.88% had>10,000 V. parahaemolyticus cells/g in TSA whereas it was 18.75% in case of TCBS. All the samples showed the presence of total V. parahaemolyticus in TSA and 97% in the case of TCBS. Interestingly, results of the trh+V. parahaemolyticus samples were quite high while using TCBS plates (62.5%) as compared to TSA (43.75%). However, the cell numbers obtained from TSA were higher than from TCBS. Several yellow colonies on TCBS turned out to be V. parahaemolyticus using colony hybridization, which was further confirmed by PCR and sucrose utilization test. Colony hybridization using DIG-labeled probe was found to be highly sensitive and could differentiate and enumerate pathogenic and non-pathogenic strains of V. parahaemolyticus. Since traditional methods are not only labor-intensive and time-consuming but also less sensitive, colony hybridization using DIG-labeled probes would be a useful alternative for the enumeration of V. parahaemolyticus in naturally contaminated seafood." @default.
• W2904176836 created "2018-12-22" @default.
• W2904176836 creator A5003373296 @default.
• W2904176836 creator A5014445801 @default.
• W2904176836 creator A5043005161 @default.
• W2904176836 creator A5059484070 @default.
• W2904176836 creator A5089393967 @default.
• W2904176836 date "2019-02-01" @default.
• W2904176836 modified "2023-09-25" @default.
• W2904176836 title "Comparative performance of TCBS and TSA for the enumeration of trh+ Vibrio parahaemolyticus by direct colony hybridization" @default.
• W2904176836 cites W1533736293 @default.
• W2904176836 cites W1564935200 @default.
• W2904176836 cites W1608243413 @default.
• W2904176836 cites W1894869549 @default.
• W2904176836 cites W1968656291 @default.
• W2904176836 cites W1973048997 @default.
• W2904176836 cites W1976045400 @default.
• W2904176836 cites W1982921550 @default.
• W2904176836 cites W1987071695 @default.
• W2904176836 cites W1995223261 @default.
• W2904176836 cites W2001315702 @default.
• W2904176836 cites W2002085031 @default.
• W2904176836 cites W2011855852 @default.
• W2904176836 cites W2049296332 @default.
• W2904176836 cites W2053570689 @default.
• W2904176836 cites W2057212751 @default.
• W2904176836 cites W2063098757 @default.
• W2904176836 cites W2066077786 @default.
• W2904176836 cites W2081088027 @default.
• W2904176836 cites W2084901406 @default.
• W2904176836 cites W2086178060 @default.
• W2904176836 cites W2098180290 @default.
• W2904176836 cites W2105294782 @default.
• W2904176836 cites W2106444703 @default.
• W2904176836 cites W2107261863 @default.
• W2904176836 cites W2128591134 @default.
• W2904176836 cites W2143853656 @default.
• W2904176836 cites W2153422894 @default.
• W2904176836 cites W2155936539 @default.
• W2904176836 cites W2156137233 @default.
• W2904176836 cites W2170492894 @default.
• W2904176836 cites W2330026425 @default.
• W2904176836 cites W2398522084 @default.
• W2904176836 cites W2580034160 @default.
• W2904176836 cites W2777230175 @default.
• W2904176836 cites W2784142300 @default.
• W2904176836 cites W2789951868 @default.
• W2904176836 cites W2791066271 @default.
• W2904176836 doi "https://doi.org/10.1016/j.mimet.2018.12.020" @default.
• W2904176836 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30578890" @default.
• W2904176836 hasPublicationYear "2019" @default.
• W2904176836 type Work @default.
• W2904176836 sameAs 2904176836 @default.
• W2904176836 citedByCount "10" @default.
• W2904176836 countsByYear W29041768362019 @default.
• W2904176836 countsByYear W29041768362020 @default.
• W2904176836 countsByYear W29041768362021 @default.
• W2904176836 countsByYear W29041768362023 @default.
• W2904176836 crossrefType "journal-article" @default.
• W2904176836 hasAuthorship W2904176836A5003373296 @default.
• W2904176836 hasAuthorship W2904176836A5014445801 @default.
• W2904176836 hasAuthorship W2904176836A5043005161 @default.
• W2904176836 hasAuthorship W2904176836A5059484070 @default.
• W2904176836 hasAuthorship W2904176836A5089393967 @default.
• W2904176836 hasConcept C114614502 @default.
• W2904176836 hasConcept C153911025 @default.
• W2904176836 hasConcept C156340839 @default.
• W2904176836 hasConcept C2776154503 @default.
• W2904176836 hasConcept C2776575720 @default.
• W2904176836 hasConcept C2778660310 @default.
• W2904176836 hasConcept C33923547 @default.
• W2904176836 hasConcept C523546767 @default.
• W2904176836 hasConcept C54355233 @default.
• W2904176836 hasConcept C86803240 @default.
• W2904176836 hasConcept C89423630 @default.
• W2904176836 hasConceptScore W2904176836C114614502 @default.
• W2904176836 hasConceptScore W2904176836C153911025 @default.
• W2904176836 hasConceptScore W2904176836C156340839 @default.
• W2904176836 hasConceptScore W2904176836C2776154503 @default.
• W2904176836 hasConceptScore W2904176836C2776575720 @default.
• W2904176836 hasConceptScore W2904176836C2778660310 @default.
• W2904176836 hasConceptScore W2904176836C33923547 @default.
• W2904176836 hasConceptScore W2904176836C523546767 @default.
• W2904176836 hasConceptScore W2904176836C54355233 @default.
• W2904176836 hasConceptScore W2904176836C86803240 @default.
• W2904176836 hasConceptScore W2904176836C89423630 @default.
• W2904176836 hasLocation W29041768361 @default.
• W2904176836 hasLocation W29041768362 @default.
• W2904176836 hasOpenAccess W2904176836 @default.
• W2904176836 hasPrimaryLocation W29041768361 @default.
• W2904176836 hasRelatedWork W1481677584 @default.
• W2904176836 hasRelatedWork W1504909894 @default.
• W2904176836 hasRelatedWork W1508227265 @default.
• W2904176836 hasRelatedWork W1548703127 @default.
• W2904176836 hasRelatedWork W1606090530 @default.
• W2904176836 hasRelatedWork W2103480494 @default.
• W2904176836 hasRelatedWork W2158235388 @default.
• W2904176836 hasRelatedWork W2161126230 @default.

• next