Matches in SemOpenAlex for { <https://semopenalex.org/work/W2904183890> ?p ?o ?g. }
- W2904183890 endingPage "24" @default.
- W2904183890 startingPage "16" @default.
- W2904183890 abstract "Piericidin A1, a member of ɑ-pyridone antibiotic, exhibits various biological activities such as antimicrobial, antifungal, and antitumor properties and possesses potent respiration-inhibitory activity against insects due to its competitive binding capacity to mitochondrial complex I. The biosynthetic pathway of piericidin A1 has been reported in Streptomyces piomogeues var. Hangzhouwanensis, while the regulatory mechanism remains poorly understood. In this study, a Streptomyces antibiotic regulatory protein (SARP) family transcriptional regulator PieR was characterized. Genetic disruption and complementation manipulations revealed that PieR positively regulated the production of piericidin A1. Moreover, the overexpression of pieR contributed to the improvement of piericidin A1 productivity. The real-time quantitative PCR (RT-qPCR) was carried out and the data showed that pieR stimulated the transcription of all the biosynthesis-related genes for piericidin A1. In order to explore the regulatory mechanism, electrophoresis mobility shift assays (EMSA) and DNase I footprinting experiments have been conducted. A protected region covering 50 nucleotides within the upstream region of pieR was identified and two 5-nt direct repeat sequences (5′-CCGGA-3′) in the protected region were found. These findings, taken together, set stage for transcriptional control engineering in the view of optimizing piericidin A1 production and thus provide a viable potent route for the construction of strains with high productivity." @default.
- W2904183890 created "2018-12-22" @default.
- W2904183890 creator A5000510528 @default.
- W2904183890 creator A5022201090 @default.
- W2904183890 creator A5030374830 @default.
- W2904183890 creator A5043764158 @default.
- W2904183890 creator A5045329487 @default.
- W2904183890 creator A5058369517 @default.
- W2904183890 creator A5072315367 @default.
- W2904183890 creator A5089035316 @default.
- W2904183890 date "2019-03-01" @default.
- W2904183890 modified "2023-10-15" @default.
- W2904183890 title "Characterization of the positive SARP family regulator PieR for improving piericidin A1 production in Streptomyces piomogeues var. Hangzhouwanensis" @default.
- W2904183890 cites W1964902920 @default.
- W2904183890 cites W1970545310 @default.
- W2904183890 cites W1972639501 @default.
- W2904183890 cites W1975905247 @default.
- W2904183890 cites W1992423271 @default.
- W2904183890 cites W1994986552 @default.
- W2904183890 cites W1995995599 @default.
- W2904183890 cites W1996214930 @default.
- W2904183890 cites W1998561695 @default.
- W2904183890 cites W1999552495 @default.
- W2904183890 cites W2007324875 @default.
- W2904183890 cites W2018847934 @default.
- W2904183890 cites W2019382774 @default.
- W2904183890 cites W2023950913 @default.
- W2904183890 cites W2032554191 @default.
- W2904183890 cites W2038126749 @default.
- W2904183890 cites W2039947832 @default.
- W2904183890 cites W2044431824 @default.
- W2904183890 cites W2047168915 @default.
- W2904183890 cites W2056553121 @default.
- W2904183890 cites W2056694217 @default.
- W2904183890 cites W2062139058 @default.
- W2904183890 cites W2065704659 @default.
- W2904183890 cites W2074548324 @default.
- W2904183890 cites W2087535192 @default.
- W2904183890 cites W2097501896 @default.
- W2904183890 cites W2097725846 @default.
- W2904183890 cites W2106601384 @default.
- W2904183890 cites W2106882534 @default.
- W2904183890 cites W2108153887 @default.
- W2904183890 cites W2116137883 @default.
- W2904183890 cites W2132632499 @default.
- W2904183890 cites W2153584471 @default.
- W2904183890 cites W2166346704 @default.
- W2904183890 cites W2166831644 @default.
- W2904183890 cites W2171555414 @default.
- W2904183890 cites W2201496207 @default.
- W2904183890 cites W2256742438 @default.
- W2904183890 cites W2300370745 @default.
- W2904183890 cites W2337517412 @default.
- W2904183890 cites W2418691443 @default.
- W2904183890 cites W2763301473 @default.
- W2904183890 cites W2773628103 @default.
- W2904183890 cites W2896241274 @default.
- W2904183890 cites W598145491 @default.
- W2904183890 doi "https://doi.org/10.1016/j.synbio.2018.12.002" @default.
- W2904183890 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6290260" @default.
- W2904183890 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30560207" @default.
- W2904183890 hasPublicationYear "2019" @default.
- W2904183890 type Work @default.
- W2904183890 sameAs 2904183890 @default.
- W2904183890 citedByCount "16" @default.
- W2904183890 countsByYear W29041838902019 @default.
- W2904183890 countsByYear W29041838902020 @default.
- W2904183890 countsByYear W29041838902021 @default.
- W2904183890 countsByYear W29041838902022 @default.
- W2904183890 crossrefType "journal-article" @default.
- W2904183890 hasAuthorship W2904183890A5000510528 @default.
- W2904183890 hasAuthorship W2904183890A5022201090 @default.
- W2904183890 hasAuthorship W2904183890A5030374830 @default.
- W2904183890 hasAuthorship W2904183890A5043764158 @default.
- W2904183890 hasAuthorship W2904183890A5045329487 @default.
- W2904183890 hasAuthorship W2904183890A5058369517 @default.
- W2904183890 hasAuthorship W2904183890A5072315367 @default.
- W2904183890 hasAuthorship W2904183890A5089035316 @default.
- W2904183890 hasBestOaLocation W29041838901 @default.
- W2904183890 hasConcept C104317684 @default.
- W2904183890 hasConcept C127716648 @default.
- W2904183890 hasConcept C138885662 @default.
- W2904183890 hasConcept C179926584 @default.
- W2904183890 hasConcept C188082640 @default.
- W2904183890 hasConcept C21592294 @default.
- W2904183890 hasConcept C27153228 @default.
- W2904183890 hasConcept C2779920670 @default.
- W2904183890 hasConcept C41895202 @default.
- W2904183890 hasConcept C523546767 @default.
- W2904183890 hasConcept C54355233 @default.
- W2904183890 hasConcept C6929976 @default.
- W2904183890 hasConcept C86339819 @default.
- W2904183890 hasConcept C86803240 @default.
- W2904183890 hasConceptScore W2904183890C104317684 @default.
- W2904183890 hasConceptScore W2904183890C127716648 @default.
- W2904183890 hasConceptScore W2904183890C138885662 @default.
- W2904183890 hasConceptScore W2904183890C179926584 @default.
- W2904183890 hasConceptScore W2904183890C188082640 @default.