FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2904232508> ?p ?o ?g. }

• W2904232508 endingPage "612" @default.
• W2904232508 startingPage "612" @default.
• W2904232508 abstract "The molecular mechanisms of translation are highly conserved in all organisms indicative of a single evolutionary origin. This includes the molecular interactions of tRNAs with their cognate aminoacyl-tRNA synthetase, which must be precise to ensure the specificity of the process. For many tRNAs, the anticodon is a major component of the specificity. This is not the case for the aminoacylation of alanine and serine to their cognate tRNAs. Rather, aminoacylation relies on other features of the tRNA. For tRNASer, a key specificity feature is the variable arm, which is positioned between the anticodon arm and the T-arm. The variable arm is conserved from yeast to human. This work was initiated to determine if the structure/function of tRNASer has been conserved from Saccharomycescerevisiae to human. We did this by detecting mistranslation in yeast cells with tRNASer derivatives having the UGA anticodon converted to UGG for proline. Despite being nearly identical in everything except the acceptor stem, human tRNASer is less active than yeast tRNASer. A chimeric tRNA with the human acceptor stem and other sequences from the yeast molecule acts similarly to the human tRNASer. The 3:70 base pair in the acceptor stem (C:G in yeast and A:U in humans) is a prime determinant of the specificity. Consistent with the functional difference of yeast and human tRNASer resulting from subtle changes in the specificity of their respective SerRS enzymes, the functionality of the human and chimeric tRNASerUGG molecules was enhanced when human SerRS was introduced into yeast. Residues in motif 2 of the aminoacylation domain of SerRS likely participated in the species-specific differences. Trp290 in yeast SerRS (Arg313 in humans) found in motif 2 is proximal to base 70 in models of the tRNA-synthetase interaction. Altering this motif 2 sequence of hSerRS to the yeast sequence decreases the activity of the human enzyme with human tRNASer, supporting the coadaptation of motif 2 loop⁻acceptor stem interactions." @default.
• W2904232508 created "2018-12-22" @default.
• W2904232508 creator A5008379091 @default.
• W2904232508 creator A5020938159 @default.
• W2904232508 creator A5026215636 @default.
• W2904232508 creator A5027317840 @default.
• W2904232508 creator A5069010163 @default.
• W2904232508 creator A5086149558 @default.
• W2904232508 date "2018-12-07" @default.
• W2904232508 modified "2023-10-14" @default.
• W2904232508 title "Acceptor Stem Differences Contribute to Species-Specific Use of Yeast and Human tRNASer" @default.
• W2904232508 cites W1489489167 @default.
• W2904232508 cites W1772521583 @default.
• W2904232508 cites W1797223236 @default.
• W2904232508 cites W1965706326 @default.
• W2904232508 cites W1967666740 @default.
• W2904232508 cites W1974959097 @default.
• W2904232508 cites W1977211785 @default.
• W2904232508 cites W1980057138 @default.
• W2904232508 cites W1980548970 @default.
• W2904232508 cites W1981529363 @default.
• W2904232508 cites W1990793025 @default.
• W2904232508 cites W1991697282 @default.
• W2904232508 cites W1994671725 @default.
• W2904232508 cites W1997985924 @default.
• W2904232508 cites W2000577570 @default.
• W2904232508 cites W2000789682 @default.
• W2904232508 cites W2005894130 @default.
• W2904232508 cites W2007345851 @default.
• W2904232508 cites W2011699532 @default.
• W2904232508 cites W2019994138 @default.
• W2904232508 cites W2021769293 @default.
• W2904232508 cites W2025362583 @default.
• W2904232508 cites W2027804115 @default.
• W2904232508 cites W2033843196 @default.
• W2904232508 cites W2035391529 @default.
• W2904232508 cites W2039698363 @default.
• W2904232508 cites W2041418072 @default.
• W2904232508 cites W2051729614 @default.
• W2904232508 cites W2051992774 @default.
• W2904232508 cites W2057481603 @default.
• W2904232508 cites W2057781025 @default.
• W2904232508 cites W2057892106 @default.
• W2904232508 cites W2062660287 @default.
• W2904232508 cites W2070222786 @default.
• W2904232508 cites W2083976355 @default.
• W2904232508 cites W2093109164 @default.
• W2904232508 cites W2094406540 @default.
• W2904232508 cites W2101176300 @default.
• W2904232508 cites W2108135447 @default.
• W2904232508 cites W2110450773 @default.
• W2904232508 cites W2118878243 @default.
• W2904232508 cites W2122602899 @default.
• W2904232508 cites W2127073167 @default.
• W2904232508 cites W2132315340 @default.
• W2904232508 cites W2137154596 @default.
• W2904232508 cites W2139305314 @default.
• W2904232508 cites W2140885472 @default.
• W2904232508 cites W2154457088 @default.
• W2904232508 cites W2155439940 @default.
• W2904232508 cites W2164709221 @default.
• W2904232508 cites W2170024651 @default.
• W2904232508 cites W2178112091 @default.
• W2904232508 cites W2223836536 @default.
• W2904232508 cites W2345451516 @default.
• W2904232508 cites W2559018695 @default.
• W2904232508 cites W2581302954 @default.
• W2904232508 cites W2620574740 @default.
• W2904232508 cites W26440169 @default.
• W2904232508 cites W2797062600 @default.
• W2904232508 cites W2804822363 @default.
• W2904232508 cites W4241480733 @default.
• W2904232508 doi "https://doi.org/10.3390/genes9120612" @default.
• W2904232508 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6316282" @default.
• W2904232508 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30544642" @default.
• W2904232508 hasPublicationYear "2018" @default.
• W2904232508 type Work @default.
• W2904232508 sameAs 2904232508 @default.
• W2904232508 citedByCount "11" @default.
• W2904232508 countsByYear W29042325082019 @default.
• W2904232508 countsByYear W29042325082020 @default.
• W2904232508 countsByYear W29042325082021 @default.
• W2904232508 countsByYear W29042325082022 @default.
• W2904232508 crossrefType "journal-article" @default.
• W2904232508 hasAuthorship W2904232508A5008379091 @default.
• W2904232508 hasAuthorship W2904232508A5020938159 @default.
• W2904232508 hasAuthorship W2904232508A5026215636 @default.
• W2904232508 hasAuthorship W2904232508A5027317840 @default.
• W2904232508 hasAuthorship W2904232508A5069010163 @default.
• W2904232508 hasAuthorship W2904232508A5086149558 @default.
• W2904232508 hasBestOaLocation W29042325081 @default.
• W2904232508 hasConcept C104317684 @default.
• W2904232508 hasConcept C151238385 @default.
• W2904232508 hasConcept C153957851 @default.
• W2904232508 hasConcept C167625842 @default.
• W2904232508 hasConcept C199216141 @default.
• W2904232508 hasConcept C2777576037 @default.
• W2904232508 hasConcept C2779222958 @default.

• next