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- W2904248259 abstract "High-throughput-sequencing-based marine microbiome profiling is rapidly expanding and changing how we study the oceans. Although powerful, the technique is not fully quantitative; it provides taxon counts only in relative abundances. In order to address this issue, we present a method to quantitatively estimate microbial abundances per unit volume of seawater filtered by spiking known amounts of internal DNA standards into each sample. We validated this method by comparing the calculated abundances to other independent estimates, including chemical markers (pigments) and total bacterial cell counts by flow cytometry. The internal standard approach allows us to quantitatively estimate and compare marine microbial community profiles, with important implications for linking environmental microbiomes to quantitative processes such as metabolic and biogeochemical rates." @default.
- W2904248259 created "2018-12-22" @default.
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- W2904248259 date "2019-03-01" @default.
- W2904248259 modified "2023-10-15" @default.
- W2904248259 title "Towards Quantitative Microbiome Community Profiling Using Internal Standards" @default.
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- W2904248259 doi "https://doi.org/10.1128/aem.02634-18" @default.
- W2904248259 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6384117" @default.
- W2904248259 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30552195" @default.
- W2904248259 hasPublicationYear "2019" @default.
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