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• W2904281134 abstract "p53 is a tumor suppressor gene involved mainly in the regulation of the G1/S cell cycle phase, DNA repair, and senescence. Although p53 is frequently altered in human cancer, the consequences of its depletion in human embryonic stem cells (hESCs) are unknown. We generated NERCe003-A-3, a p53 knockout hESC line, from the normal NERCe003-A hESC line by using CRISPR/Cas9 editing. This cell line maintained a normal 46, XY karyotype. Further analysis suggested that the cells expressed pluripotency-related markers and had the capacity to differentiate in vitro into derivatives of all three germ layers." @default.
• W2904281134 created "2018-12-22" @default.
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• W2904281134 date "2019-01-01" @default.
• W2904281134 modified "2023-09-27" @default.
• W2904281134 title "Generation of NERCe003-A-3, a p53 compound heterozygous mutation human embryonic stem cell line, by CRISPR/Cas9 editing" @default.
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• W2904281134 doi "https://doi.org/10.1016/j.scr.2018.101371" @default.
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