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- W2904328045 abstract "Objective: This study aimed to isolate gelatin, determine its characteristics, and obtain analytical methods optimum for the determination of glycine,proline, and hydroxyproline levels in bovine gelatin.Methods: Bovine hide was hydrolyzed using 2% sodium hydroxide at an extraction temperature of 70°C for 3 h and a drying temperature of 60°C.Then, the gelatin extracts were evaluated using an organoleptic test; Fourier transform infrared analysis; pH measurement; and ash content, moisturecontent, and viscosity tests. Optimum analysis conditions for the determination of glycine, proline, and hydroxyproline levels in bovine gelatin usinghigh-performance liquid chromatography with a fluorescence detector were as follows: Excitation wavelength, 265 nm; emission wavelength, 320 nm;mobile phase composition of acetic buffer: acetonitrile, 55:45; flow rate, 0.8 mL/min; C18 column with length 250 mm and inner diameter 4.6 mm;and particle size, 5 μm. Derivatization of amino acids was performed using the reagent 9-fluorenylmethoxycarbonyl chloride.Results: The results showed average levels of glycine, proline, and hydroxyproline in bovine gelatin, i.e., 25.10±0.09%, 14.28±0.11%, and 13.0±0.05%,respectively.Conclusion: The optimum conditions for bovine gelatin hydrolysis included HCl with heating for 22 h at 110°C. The hydroxyproline, glycine, andproline levels obtained for analysis in bovine gelatin samples were 13.50±0.05%, 25.11±0.09%, and 14.28±0.11%, respectively." @default.
- W2904328045 created "2018-12-22" @default.
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- W2904328045 date "2018-12-20" @default.
- W2904328045 modified "2023-10-18" @default.
- W2904328045 title "ISOLATION AND CHARACTERISTICS OF BOVINE SKIN GELATIN AND ANALYSIS OF GLYCINE, PROLINE, AND HYDROXYPROLINE BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHYFLUORESCENCE" @default.
- W2904328045 doi "https://doi.org/10.22159/ijap.2018.v10s1.60" @default.
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