Matches in SemOpenAlex for { <https://semopenalex.org/work/W2904330102> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W2904330102 abstract "Infectious Bronchitis (IB) is highly contagious disease of commercial poultry causing substantial economic loses by producing poor quality meat in broilers and effecting production in breeder birds. The causative agent has been reported as most hazardous pathogen among other infectious agent even after being immunized with multi-variant strain vaccine. Currently, different strain such as H-120, 4/91 and D274 have been used extensively for immunoprophylaxis against velogenic strain across Pakistan with minimal protection reported. In current study PCR analysis was used to investigate the molecular nature of IB isolates from Punjab and Sind province of Pakistan in 2016 epidemics. Total of 100 tracheal samples were considered for virus inoculation in 10 days old chicken embryonated eggs. The IBV infected amniotic fluid was neutralized with monoclonal antisera of H-120, 4/91 and D274 strains. The IBV screened samples were subjected for RNA extraction and subsequent to PCR using type specific primer of each strain. The amplified product of 840 bp was sequenced through Sanger sequencing. On the basis of PCR results, four similar amplified products from both regions were obtained showing similarities in agarose gel electrophoresis, but they differ from each other on the basis of nucleotides sequence. Phylogenetic analysis revealed that nucleotide sequences of isolates from Karachi were similar to the IBV H-120, Mass-41 and Connecticut 46 reference strains. Whereas, isolates from the Punjab province are analogous to the Mans-2, Mans-3, 9/41(UK) but did not show significant similarity with other reference strain. Therefore, it is recommended that use of M-41 and H-120 in vaccine production could be effective measure against velogenic infectious agent in Sindh particularly in Karachi, whereas, it would be better to incorporate either of the variant GQ281656.1, AY279533.1 in vaccine because of their highest level of resemblance with genetically sequenced isolates from Lahore and its surroundings." @default.
- W2904330102 created "2018-12-22" @default.
- W2904330102 creator A5005084813 @default.
- W2904330102 creator A5012574826 @default.
- W2904330102 creator A5033565947 @default.
- W2904330102 creator A5040592934 @default.
- W2904330102 creator A5044703046 @default.
- W2904330102 creator A5057802073 @default.
- W2904330102 creator A5076761008 @default.
- W2904330102 date "2018-01-01" @default.
- W2904330102 modified "2023-09-28" @default.
- W2904330102 title "Molecular Detection and Sequencing for S1 Glycoprotein Gene of Bronchitis Virus of 2016 Epidemic from Sindh and Punjab" @default.
- W2904330102 doi "https://doi.org/10.4236/abb.2018.912044" @default.
- W2904330102 hasPublicationYear "2018" @default.
- W2904330102 type Work @default.
- W2904330102 sameAs 2904330102 @default.
- W2904330102 citedByCount "0" @default.
- W2904330102 crossrefType "journal-article" @default.
- W2904330102 hasAuthorship W2904330102A5005084813 @default.
- W2904330102 hasAuthorship W2904330102A5012574826 @default.
- W2904330102 hasAuthorship W2904330102A5033565947 @default.
- W2904330102 hasAuthorship W2904330102A5040592934 @default.
- W2904330102 hasAuthorship W2904330102A5044703046 @default.
- W2904330102 hasAuthorship W2904330102A5057802073 @default.
- W2904330102 hasAuthorship W2904330102A5076761008 @default.
- W2904330102 hasBestOaLocation W29043301021 @default.
- W2904330102 hasConcept C104317684 @default.
- W2904330102 hasConcept C129470801 @default.
- W2904330102 hasConcept C159047783 @default.
- W2904330102 hasConcept C2522874641 @default.
- W2904330102 hasConcept C2776460866 @default.
- W2904330102 hasConcept C2777020439 @default.
- W2904330102 hasConcept C2778271344 @default.
- W2904330102 hasConcept C2993325435 @default.
- W2904330102 hasConcept C51679486 @default.
- W2904330102 hasConcept C54355233 @default.
- W2904330102 hasConcept C76818968 @default.
- W2904330102 hasConcept C86803240 @default.
- W2904330102 hasConcept C89423630 @default.
- W2904330102 hasConceptScore W2904330102C104317684 @default.
- W2904330102 hasConceptScore W2904330102C129470801 @default.
- W2904330102 hasConceptScore W2904330102C159047783 @default.
- W2904330102 hasConceptScore W2904330102C2522874641 @default.
- W2904330102 hasConceptScore W2904330102C2776460866 @default.
- W2904330102 hasConceptScore W2904330102C2777020439 @default.
- W2904330102 hasConceptScore W2904330102C2778271344 @default.
- W2904330102 hasConceptScore W2904330102C2993325435 @default.
- W2904330102 hasConceptScore W2904330102C51679486 @default.
- W2904330102 hasConceptScore W2904330102C54355233 @default.
- W2904330102 hasConceptScore W2904330102C76818968 @default.
- W2904330102 hasConceptScore W2904330102C86803240 @default.
- W2904330102 hasConceptScore W2904330102C89423630 @default.
- W2904330102 hasLocation W29043301021 @default.
- W2904330102 hasOpenAccess W2904330102 @default.
- W2904330102 hasPrimaryLocation W29043301021 @default.
- W2904330102 hasRelatedWork W1948644052 @default.
- W2904330102 hasRelatedWork W1973222961 @default.
- W2904330102 hasRelatedWork W1978757788 @default.
- W2904330102 hasRelatedWork W2002188701 @default.
- W2904330102 hasRelatedWork W2024607571 @default.
- W2904330102 hasRelatedWork W2024793384 @default.
- W2904330102 hasRelatedWork W2024945322 @default.
- W2904330102 hasRelatedWork W2030779555 @default.
- W2904330102 hasRelatedWork W2129437035 @default.
- W2904330102 hasRelatedWork W2189217962 @default.
- W2904330102 hasRelatedWork W2227505077 @default.
- W2904330102 hasRelatedWork W2276062549 @default.
- W2904330102 hasRelatedWork W2356608672 @default.
- W2904330102 hasRelatedWork W2373414017 @default.
- W2904330102 hasRelatedWork W2378498236 @default.
- W2904330102 hasRelatedWork W2953111230 @default.
- W2904330102 hasRelatedWork W2969441774 @default.
- W2904330102 hasRelatedWork W2994869925 @default.
- W2904330102 hasRelatedWork W3026911492 @default.
- W2904330102 hasRelatedWork W3097488757 @default.
- W2904330102 isParatext "false" @default.
- W2904330102 isRetracted "false" @default.
- W2904330102 magId "2904330102" @default.
- W2904330102 workType "article" @default.