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- W2904333631 startingPage "4025" @default.
- W2904333631 abstract "The neural crest (NC) comprises a multipotent cell population that produces peripheral neurons, cartilage, and smooth muscle cells, among other phenotypes. The participation of Hes1 and Msx1 when expressed in mouse embryonic stem cells (mESCs) undergoing NC differentiation is unexplored. In this work, we generated stable mESCs transfected with constructs encoding chimeric proteins in which the ligand binding domain of glucocorticoid receptor (GR), which is translocated to the nucleus by dexamethasone addition, is fused to either Hes1 (HGR) or Msx1 (MGR), as well as double-transgenic cells (HGR+MGR). These lines continued to express pluripotency markers. Upon NC differentiation, all lines exhibited significantly decreased Sox2 expression and upregulated Sox9, Snai1, and Msx1 expression, indicating NC commitment. Dexamethasone was added to induce nuclear translocation of the chimeric proteins. We found that Collagen IIa transcripts were increased in MGR cells, whereas coactivation of HGR+MGR caused a significant increase in Smooth muscle actin (α-Sma) transcripts. Immunostaining showed that activation in HGR+MGR cells induced higher proportions of β-TUBULIN III⁺, α-SMA⁺ and COL2A1⁺ cells. These findings indicate that nuclear translocation of MSX-1, alone or in combination with HES-1, produce chondrocyte-like cells, and simultaneous activation of HES-1 and MSX-1 increases the generation of smooth muscle and neuronal cells." @default.
- W2904333631 created "2018-12-22" @default.
- W2904333631 creator A5017666148 @default.
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- W2904333631 creator A5043206377 @default.
- W2904333631 creator A5091607545 @default.
- W2904333631 creator A5091772281 @default.
- W2904333631 date "2018-12-13" @default.
- W2904333631 modified "2023-10-18" @default.
- W2904333631 title "Activation of Hes1 and Msx1 in Transgenic Mouse Embryonic Stem Cells Increases Differentiation into Neural Crest Derivatives" @default.
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- W2904333631 doi "https://doi.org/10.3390/ijms19124025" @default.
- W2904333631 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6321090" @default.
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- W2904333631 hasPublicationYear "2018" @default.
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