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- W2904354959 abstract "DNA damage repair proteins form foci in response to DNA damaging agents. The efficiency and integrity of the DNA repair pathway of a particular eukaryotic (mutant) strain is usually determined by the number of foci formed compared with their wild-type counterpart. Conventionally, focus number is determined visually, and this low accuracy may obscure the identification of a weaker phenotype, particularly when the output is low. Here, using the homologous recombination protein Rhp54 as an example, we present a protocol that can increase the consistency of foci identification among samples and can significantly improve the efficiency of foci quantification for large sample sizes. A similar method can be applied to other foci-forming proteins." @default.
- W2904354959 created "2018-12-22" @default.
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- W2904354959 date "2018-01-01" @default.
- W2904354959 modified "2023-09-25" @default.
- W2904354959 title "Systematic Quantification of GFP-tagged Protein Foci in Schizosaccharomyces pombe Nuclei" @default.
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- W2904354959 doi "https://doi.org/10.21769/bioprotoc.3117" @default.
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