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- W2904367185 abstract "Abstract The aim of this study was to evaluate the effect of vanillin as a QS inhibitor at sub-minimal inhibitory concentrations (sub-MICs) against fish spoilage by Hafnia alvei. Biofilm formation, swimming and swarming activity, and production of acyl-homoserine lactones (AHLs) were measured. In addition, analysis of quorum sensing (QS)-related genes by real-time quantitative PCR (RT-qPCR) and molecular docking was performed to better understand the inhibitory mechanism of vanillin. The results indicated that sub-MICs of vanillin induced remarkable reduction in violacein production (59.0%) and biofilm formation (68.5%) along with decreased swimming ability, and reduced expression levels of halI and halR. Moreover, the results of molecular docking revealed that vanillin and C6-HSL could form 2 hydrogen bonds with the LuxR protein (HalR), suggesting a potential competitive tendency between them. Thus, our study provides a basic inhibition mechanism of vanillin and suggests its prospective application as a QS inhibitor." @default.
- W2904367185 created "2018-12-22" @default.
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- W2904367185 date "2019-03-01" @default.
- W2904367185 modified "2023-09-29" @default.
- W2904367185 title "Inhibitory effect of vanillin on the virulence factors and biofilm formation of Hafnia alvei" @default.
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- W2904367185 doi "https://doi.org/10.1016/j.lwt.2018.12.038" @default.
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