Matches in SemOpenAlex for { <https://semopenalex.org/work/W2904371685> ?p ?o ?g. }
- W2904371685 abstract "The purpose of this study was to investigate the role of malignant ascites tumor microenvironment in ovarian cancer progression and chemoresistance.A total of 45 patients with ovarian cancer and three benign ascites were collected at the time of clinical intervention. Ascites cholesterol levels were quantitated using cholesterol quantitation kit and recurrence free survival (RFS) of ovarian cancer patients were collected. The sensitivity of ovarian cancer cells to cisplatin (CDDP) and paclitaxel (PAC) were assessed by viability assay, flow cytometry and protein expression. Receiver operating characteristics (ROC) curve and Youden index analysis were applied to calculate the optimal cut-off values for ascites cholesterol. Kaplan-Meier curve were applied to compare RFS between high and low ascites cholesterol levels in ovarian cancer patients.Here we show that cholesterol is elevated in malignant ascites and modulates the sensitivity of ovarian cancer cells to CDDP and PAC by upregulating the expression of drug efflux pump proteins, ABCG2 and MDR1, together with upregulation of LXRɑ/β, the cholesterol receptor. Transfection of LXRɑ/β siRNA inhibited cholesterol-induced chemoresistance and upregulation of MDR1. In addition, the cholesterol level in malignant ascites was negatively correlated with number of CDDP-induced apoptotic cell death, but not with that of PAC-induced apoptotic cell death. Cholesterol depletion by methyl beta cyclodextrin (MβCD) inhibited malignant ascites-induced chemoresistance to CDDP and upregulation of MDR1 and LXRɑ/β. For patients with ovarian cancer, high cholesterol level in malignant ascites correlated with short RFS.High cholesterol in malignant ascites contributes to poor prognosis in ovarian cancer patients, partly by contributing to multidrug resistance through upregulation of MDR1 via activation of LXRɑ/β." @default.
- W2904371685 created "2018-12-22" @default.
- W2904371685 creator A5004406086 @default.
- W2904371685 creator A5038606972 @default.
- W2904371685 creator A5053139445 @default.
- W2904371685 creator A5055872689 @default.
- W2904371685 date "2018-12-01" @default.
- W2904371685 modified "2023-10-11" @default.
- W2904371685 title "Activation of LXRɑ/β by cholesterol in malignant ascites promotes chemoresistance in ovarian cancer" @default.
- W2904371685 cites W113851183 @default.
- W2904371685 cites W1511099714 @default.
- W2904371685 cites W1978082964 @default.
- W2904371685 cites W1982542571 @default.
- W2904371685 cites W1990394752 @default.
- W2904371685 cites W1991953785 @default.
- W2904371685 cites W1998154098 @default.
- W2904371685 cites W2001265443 @default.
- W2904371685 cites W2005849691 @default.
- W2904371685 cites W2006434892 @default.
- W2904371685 cites W2008211811 @default.
- W2904371685 cites W2009211019 @default.
- W2904371685 cites W2014393189 @default.
- W2904371685 cites W2017323433 @default.
- W2904371685 cites W2019411105 @default.
- W2904371685 cites W2028890879 @default.
- W2904371685 cites W2030361670 @default.
- W2904371685 cites W2041943343 @default.
- W2904371685 cites W2052637997 @default.
- W2904371685 cites W2054146518 @default.
- W2904371685 cites W2056772956 @default.
- W2904371685 cites W2064057764 @default.
- W2904371685 cites W2066616821 @default.
- W2904371685 cites W2076920991 @default.
- W2904371685 cites W2077255241 @default.
- W2904371685 cites W2082011949 @default.
- W2904371685 cites W2083453016 @default.
- W2904371685 cites W2091455442 @default.
- W2904371685 cites W2098918967 @default.
- W2904371685 cites W2101375502 @default.
- W2904371685 cites W2102848212 @default.
- W2904371685 cites W2103107569 @default.
- W2904371685 cites W2115954319 @default.
- W2904371685 cites W2121080406 @default.
- W2904371685 cites W2133394672 @default.
- W2904371685 cites W2135866806 @default.
- W2904371685 cites W2142083551 @default.
- W2904371685 cites W2154881851 @default.
- W2904371685 cites W2157158547 @default.
- W2904371685 cites W2235732203 @default.
- W2904371685 cites W2269087069 @default.
- W2904371685 cites W2275989970 @default.
- W2904371685 cites W2328390592 @default.
- W2904371685 cites W2407433978 @default.
- W2904371685 cites W2425132249 @default.
- W2904371685 cites W2472306545 @default.
- W2904371685 cites W2548838471 @default.
- W2904371685 cites W2576078054 @default.
- W2904371685 cites W2736018139 @default.
- W2904371685 doi "https://doi.org/10.1186/s12885-018-5152-5" @default.
- W2904371685 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6288854" @default.
- W2904371685 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30526541" @default.
- W2904371685 hasPublicationYear "2018" @default.
- W2904371685 type Work @default.
- W2904371685 sameAs 2904371685 @default.
- W2904371685 citedByCount "32" @default.
- W2904371685 countsByYear W29043716852019 @default.
- W2904371685 countsByYear W29043716852020 @default.
- W2904371685 countsByYear W29043716852021 @default.
- W2904371685 countsByYear W29043716852022 @default.
- W2904371685 countsByYear W29043716852023 @default.
- W2904371685 crossrefType "journal-article" @default.
- W2904371685 hasAuthorship W2904371685A5004406086 @default.
- W2904371685 hasAuthorship W2904371685A5038606972 @default.
- W2904371685 hasAuthorship W2904371685A5053139445 @default.
- W2904371685 hasAuthorship W2904371685A5055872689 @default.
- W2904371685 hasBestOaLocation W29043716851 @default.
- W2904371685 hasConcept C104317684 @default.
- W2904371685 hasConcept C121608353 @default.
- W2904371685 hasConcept C126322002 @default.
- W2904371685 hasConcept C127561419 @default.
- W2904371685 hasConcept C134018914 @default.
- W2904371685 hasConcept C143998085 @default.
- W2904371685 hasConcept C2778163477 @default.
- W2904371685 hasConcept C2780427987 @default.
- W2904371685 hasConcept C2780496750 @default.
- W2904371685 hasConcept C502942594 @default.
- W2904371685 hasConcept C55493867 @default.
- W2904371685 hasConcept C71924100 @default.
- W2904371685 hasConcept C86803240 @default.
- W2904371685 hasConceptScore W2904371685C104317684 @default.
- W2904371685 hasConceptScore W2904371685C121608353 @default.
- W2904371685 hasConceptScore W2904371685C126322002 @default.
- W2904371685 hasConceptScore W2904371685C127561419 @default.
- W2904371685 hasConceptScore W2904371685C134018914 @default.
- W2904371685 hasConceptScore W2904371685C143998085 @default.
- W2904371685 hasConceptScore W2904371685C2778163477 @default.
- W2904371685 hasConceptScore W2904371685C2780427987 @default.
- W2904371685 hasConceptScore W2904371685C2780496750 @default.
- W2904371685 hasConceptScore W2904371685C502942594 @default.
- W2904371685 hasConceptScore W2904371685C55493867 @default.